A putative wild-type or wild-type-like hairpin structure is required within 3' untranslated region of Senecavirus A for virus replication
Copyright © 2023 Elsevier Inc. All rights reserved..
The 3' untranslated region (UTR) of Senecavirus A (SVA) was predicted to harbor two hairpin structures, hairpin-I and -II. The former is composed of two internal loops, one terminal loop and three stem regions; the latter comprises one internal loop, one terminal loop and two stem regions. In this study, we constructed a total of nine SVA cDNA clones, which contained different point mutations within a stem-formed motif in the hairpin-I or -II, for rescuing replication-competent viruses. Only three mutants were successfully rescued and moreover genetically stable during at least five serial passages. Computer-aided prediction showed these three mutants bearing either a wild-type or a wild-type-like hairpin-I in their individual 3' UTRs. Neither wild-type nor wild-type-like hairpin-I could be computationally predicted to exist in 3' UTRs of the other six unviable "viruses". The results suggested that the wild-type or wild-type-like hairpin-I was necessary in the 3' UTR for SVA replication.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:585 |
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| Enthalten in: |
Virology - 585(2023) vom: 16. Aug., Seite 72-77 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhao, Di [VerfasserIn] |
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| Links: |
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| Themen: |
3' Untranslated Regions |
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| Anmerkungen: |
Date Completed 24.07.2023 Date Revised 25.07.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.virol.2023.05.008 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM358093562 |
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| 100 | 1 | |a Zhao, Di |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A putative wild-type or wild-type-like hairpin structure is required within 3' untranslated region of Senecavirus A for virus replication |
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| 500 | |a Date Completed 24.07.2023 | ||
| 500 | |a Date Revised 25.07.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023 Elsevier Inc. All rights reserved. | ||
| 520 | |a The 3' untranslated region (UTR) of Senecavirus A (SVA) was predicted to harbor two hairpin structures, hairpin-I and -II. The former is composed of two internal loops, one terminal loop and three stem regions; the latter comprises one internal loop, one terminal loop and two stem regions. In this study, we constructed a total of nine SVA cDNA clones, which contained different point mutations within a stem-formed motif in the hairpin-I or -II, for rescuing replication-competent viruses. Only three mutants were successfully rescued and moreover genetically stable during at least five serial passages. Computer-aided prediction showed these three mutants bearing either a wild-type or a wild-type-like hairpin-I in their individual 3' UTRs. Neither wild-type nor wild-type-like hairpin-I could be computationally predicted to exist in 3' UTRs of the other six unviable "viruses". The results suggested that the wild-type or wild-type-like hairpin-I was necessary in the 3' UTR for SVA replication | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a 3′ untranslated region | |
| 650 | 4 | |a Mutation | |
| 650 | 4 | |a Senecavirus A | |
| 650 | 4 | |a Stem | |
| 650 | 4 | |a Wild-type hairpin | |
| 650 | 4 | |a Wild-type-like hairpin | |
| 650 | 7 | |a 3' Untranslated Regions |2 NLM | |
| 650 | 7 | |a RNA, Viral |2 NLM | |
| 700 | 1 | |a Wang, Qianqian |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Mengyao |e verfasserin |4 aut | |
| 700 | 1 | |a Lyu, Liangpeng |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Shuqing |e verfasserin |4 aut | |
| 700 | 1 | |a Jiang, Yujia |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Shuning |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Fuxiao |e verfasserin |4 aut | |
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