Volumetric absorptive microsampling as an effective microsampling technique for LC-MS/MS bioanalysis of biomarkers in drug discovery
Aim: Develop and validate a volumetric absorptive microsampling (VAMS)-based LC-MS/MS method to support the bioanalysis of amino acid and carboxylic acid biomarkers in mouse whole blood. Method: Mouse whole blood was collected using a 10 μl VAMS device. The analytes in VAMS were extracted and analyzed using an LC-MS/MS method. Results: The VAMS-based LC-MS/MS assay exhibited a linearity range of 10.0-10,000 ng/ml with acceptable precision and accuracy and consistent recovery. The analyte stability in mouse whole blood VAMS was shown for 7 days at ambient conditions and at -80°C, as well as with three freeze/thaw cycles. Conclusion: A simple and robust VAMS-based LC-MS/MS method was developed and further validated for simultaneous bioanalysis of nine biomarkers in mouse whole blood.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:15 |
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| Enthalten in: |
Bioanalysis - 15(2023), 14 vom: 23. Juli, Seite 845-859 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kapadnis, Unnati [VerfasserIn] |
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| Links: |
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| Themen: |
Amino acids |
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| Anmerkungen: |
Date Completed 07.09.2023 Date Revised 07.09.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.4155/bio-2023-0059 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM358076676 |
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| 500 | |a Date Revised 07.09.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Aim: Develop and validate a volumetric absorptive microsampling (VAMS)-based LC-MS/MS method to support the bioanalysis of amino acid and carboxylic acid biomarkers in mouse whole blood. Method: Mouse whole blood was collected using a 10 μl VAMS device. The analytes in VAMS were extracted and analyzed using an LC-MS/MS method. Results: The VAMS-based LC-MS/MS assay exhibited a linearity range of 10.0-10,000 ng/ml with acceptable precision and accuracy and consistent recovery. The analyte stability in mouse whole blood VAMS was shown for 7 days at ambient conditions and at -80°C, as well as with three freeze/thaw cycles. Conclusion: A simple and robust VAMS-based LC-MS/MS method was developed and further validated for simultaneous bioanalysis of nine biomarkers in mouse whole blood | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a LC–MS/MS | |
| 650 | 4 | |a VAMS | |
| 650 | 4 | |a amino acids | |
| 650 | 4 | |a biomarkers | |
| 650 | 4 | |a carboxylic acids | |
| 650 | 4 | |a microsampling | |
| 700 | 1 | |a Locuson, Chuck |e verfasserin |4 aut | |
| 700 | 1 | |a Okamura, Heidi |e verfasserin |4 aut | |
| 700 | 1 | |a Rienzo, Gianluca De |e verfasserin |4 aut | |
| 700 | 1 | |a Cotter, Cece |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Dongwei |e verfasserin |4 aut | |
| 700 | 1 | |a Narayanaswami, Rohini |e verfasserin |4 aut | |
| 700 | 1 | |a Castro-Perez, Jose |e verfasserin |4 aut | |
| 700 | 1 | |a Marathe, Punit |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Wen-Chu |e verfasserin |4 aut | |
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