Detection of M2-Type Anti-Mitochondrial Autoantibodies against Specific Subunits in the Diagnosis of Primary Biliary Cholangitis in Patients with Discordant Results
BACKGROUND: M2-type anti-mitochondrial autoantibodies are considered the hallmark of primary biliary cholangitis and are directed mainly against the E2 subunits of the 2-oxo acid dehydrogenase complex enzymes (PDC, BCOADC and OGDC). The aim of this study was to determine whether a Dot-blot that includes these E2 subunits separately could confirm the results of methods with non-separated subunits in patients with low positive or discordant results between techniques.
METHODS: Sera of 24 patients with low positive or discordant results and of 10 patients with clear positive results by non-separated subunits methods were analyzed by Dot-blot with separated subunits.
RESULTS: Autoantibodies against E2 subunits of PDC, BCOADC or OGDC were detected in all patients, except in one case from the low positive or discordant results group, by Dot-blot with separated subunits.
CONCLUSIONS: It would be advisable to use methods that include the three E2 subunits, and a Dot-blot with separated subunits could confirm doubtful cases by non-separated assays.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
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| Enthalten in: |
Diagnostics (Basel, Switzerland) - 13(2023), 11 vom: 24. Mai |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Arnaldos-Pérez, Cristina [VerfasserIn] |
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| Links: |
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| Themen: |
AMA-M2 |
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| Anmerkungen: |
Date Revised 12.06.2023 published: Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.3390/diagnostics13111840 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM35798448X |
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| 100 | 1 | |a Arnaldos-Pérez, Cristina |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Detection of M2-Type Anti-Mitochondrial Autoantibodies against Specific Subunits in the Diagnosis of Primary Biliary Cholangitis in Patients with Discordant Results |
| 264 | 1 | |c 2023 | |
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| 500 | |a Date Revised 12.06.2023 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a BACKGROUND: M2-type anti-mitochondrial autoantibodies are considered the hallmark of primary biliary cholangitis and are directed mainly against the E2 subunits of the 2-oxo acid dehydrogenase complex enzymes (PDC, BCOADC and OGDC). The aim of this study was to determine whether a Dot-blot that includes these E2 subunits separately could confirm the results of methods with non-separated subunits in patients with low positive or discordant results between techniques | ||
| 520 | |a METHODS: Sera of 24 patients with low positive or discordant results and of 10 patients with clear positive results by non-separated subunits methods were analyzed by Dot-blot with separated subunits | ||
| 520 | |a RESULTS: Autoantibodies against E2 subunits of PDC, BCOADC or OGDC were detected in all patients, except in one case from the low positive or discordant results group, by Dot-blot with separated subunits | ||
| 520 | |a CONCLUSIONS: It would be advisable to use methods that include the three E2 subunits, and a Dot-blot with separated subunits could confirm doubtful cases by non-separated assays | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a AMA-M2 | |
| 650 | 4 | |a BCOADC | |
| 650 | 4 | |a Dot-blot | |
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| 650 | 4 | |a ELISA | |
| 650 | 4 | |a OGDC | |
| 650 | 4 | |a PDC | |
| 650 | 4 | |a autoantibodies | |
| 650 | 4 | |a indirect immunofluorescence | |
| 650 | 4 | |a primary biliary cholangitis | |
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| 700 | 1 | |a Bolos, Uma |e verfasserin |4 aut | |
| 700 | 1 | |a Domènech, Carmen |e verfasserin |4 aut | |
| 700 | 1 | |a Ballús, Judit |e verfasserin |4 aut | |
| 700 | 1 | |a Rodríguez-Tajes, Sergio |e verfasserin |4 aut | |
| 700 | 1 | |a Londoño, María Carlota |e verfasserin |4 aut | |
| 700 | 1 | |a Viñas, Odette |e verfasserin |4 aut | |
| 700 | 1 | |a Ruiz-Ortiz, Estíbaliz |e verfasserin |4 aut | |
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