Investigating Pseudomonas aeruginosa population structure and frequency of cross-infection in UK cystic fibrosis clinics - a reference laboratory perspective
Copyright © 2023. Published by Elsevier B.V..
BACKGROUND: We aimed to describe the UK Pseudomonas aeruginosa population structure amongst people with cystic fibrosis (PWCF), and to examine evidence for cross-infection.
METHODS: Variable Number Tandem Repeat (VNTR) typing was performed on 4640 isolates from 2619 PWCF received from 55 hospital laboratories between 2017 and 2019. A combination of whole genome sequence (WGS)-based analysis of four clusters from one hospital, and epidemiological analysis of shared strains in twelve hospitals evaluated cross-infection.
RESULTS: Of 2619 PWCF, 1324 (51%) harboured common clusters or known transmissible strains, while 1295 carried unique strains/those shared among small numbers of patients. Of the former, 9.5% (250 patients) harboured the Liverpool epidemic strain (LES), followed in prevalence by clone C (7.8%; 205 patients), cluster A (5%;130 patients), and cluster D (3.6%; 94 patients). WGS analysis of 10 LES isolates, 9 of cluster D and 6 isolates each of cluster A and clone C from one hospital revealed LES formed the tightest cluster (between 7 and 205 SNPs), and cluster D the loosest (between 53 and 1531 SNPs). Hospital-specific shared strains were found in some centres, although cross-infection was largely historical, with few new acquisitions. Fifty-nine PWCF (2.3%) harboured "high-risk" clones; one ST235 isolate carried a blaIMP-1 allele.
CONCLUSION: Of 2619 PWCF who had P. aeruginosa isolates submitted for VNTR, 51% harboured either common clusters or known transmissible strains, of which LES was the most common. Limited evidence of recent patient-to-patient strain transmission was found, suggesting cross-infection prevention measures and surveillance effectively reduce transmission.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:22 |
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| Enthalten in: |
Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society - 22(2023), 5 vom: 01. Sept., Seite 894-900 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kenna, Dervla T D [VerfasserIn] |
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| Links: |
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| Themen: |
Cross-infection |
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| Anmerkungen: |
Date Completed 28.11.2023 Date Revised 24.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.jcf.2023.05.017 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM357736834 |
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| 245 | 1 | 0 | |a Investigating Pseudomonas aeruginosa population structure and frequency of cross-infection in UK cystic fibrosis clinics - a reference laboratory perspective |
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| 500 | |a Date Revised 24.04.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023. Published by Elsevier B.V. | ||
| 520 | |a BACKGROUND: We aimed to describe the UK Pseudomonas aeruginosa population structure amongst people with cystic fibrosis (PWCF), and to examine evidence for cross-infection | ||
| 520 | |a METHODS: Variable Number Tandem Repeat (VNTR) typing was performed on 4640 isolates from 2619 PWCF received from 55 hospital laboratories between 2017 and 2019. A combination of whole genome sequence (WGS)-based analysis of four clusters from one hospital, and epidemiological analysis of shared strains in twelve hospitals evaluated cross-infection | ||
| 520 | |a RESULTS: Of 2619 PWCF, 1324 (51%) harboured common clusters or known transmissible strains, while 1295 carried unique strains/those shared among small numbers of patients. Of the former, 9.5% (250 patients) harboured the Liverpool epidemic strain (LES), followed in prevalence by clone C (7.8%; 205 patients), cluster A (5%;130 patients), and cluster D (3.6%; 94 patients). WGS analysis of 10 LES isolates, 9 of cluster D and 6 isolates each of cluster A and clone C from one hospital revealed LES formed the tightest cluster (between 7 and 205 SNPs), and cluster D the loosest (between 53 and 1531 SNPs). Hospital-specific shared strains were found in some centres, although cross-infection was largely historical, with few new acquisitions. Fifty-nine PWCF (2.3%) harboured "high-risk" clones; one ST235 isolate carried a blaIMP-1 allele | ||
| 520 | |a CONCLUSION: Of 2619 PWCF who had P. aeruginosa isolates submitted for VNTR, 51% harboured either common clusters or known transmissible strains, of which LES was the most common. Limited evidence of recent patient-to-patient strain transmission was found, suggesting cross-infection prevention measures and surveillance effectively reduce transmission | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Cross-infection | |
| 650 | 4 | |a Cystic fibrosis | |
| 650 | 4 | |a Liverpool epidemic strain | |
| 650 | 4 | |a Pseudomonas aeruginosa | |
| 650 | 4 | |a Whole genome sequencing | |
| 700 | 1 | |a Payne, Zoë |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, David A |e verfasserin |4 aut | |
| 700 | 1 | |a Keane, Ann-Marie |e verfasserin |4 aut | |
| 700 | 1 | |a Turton, Jack |e verfasserin |4 aut | |
| 700 | 1 | |a Zamarreño, Dania V |e verfasserin |4 aut | |
| 700 | 1 | |a Schaefer, Ulf |e verfasserin |4 aut | |
| 700 | 1 | |a Hopkins, Katie L |e verfasserin |4 aut | |
| 700 | 1 | |a Meunier, Danièle |e verfasserin |4 aut | |
| 700 | 1 | |a Dhillon, Rishi |e verfasserin |4 aut | |
| 700 | 1 | |a Duckers, Jamie |e verfasserin |4 aut | |
| 700 | 1 | |a Speight, Lorraine |e verfasserin |4 aut | |
| 700 | 1 | |a Turton, Jane F |e verfasserin |4 aut | |
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