Zika virus RNA persistence and recovery in water and wastewater : An approach for Zika virus surveillance in resource-constrained settings
Copyright © 2023. Published by Elsevier Ltd..
During the 2015-2016 Zika virus (ZIKV) epidemic in the Americas, serological cross-reactivity with other flaviviruses and relatively high costs of nucleic acid testing in the region hindered the capacity for widespread diagnostic testing. In such cases where individual testing is not feasible, wastewater monitoring approaches may offer a means of community-level public health surveillance. To inform such approaches, we characterized the persistence and recovery of ZIKV RNA in experiments where we spiked cultured ZIKV into surface water, wastewater, and a combination of both to examine the potential for detection in open sewers serving communities most affected by the ZIKV outbreak, such as those in Salvador, Bahia, Brazil. We used reverse transcription droplet digital PCR to quantify ZIKV RNA. In our persistence experiments, we found that the persistence of ZIKV RNA decreased with increasing temperature, significantly decreased in surface water versus wastewater, and significantly decreased when the initial concentration of virus was lowered by one order of magnitude. In our recovery experiments, we found higher percent recovery of ZIKV RNA in pellets versus supernatants from the same sample, higher recoveries in pellets using skimmed milk flocculation, lower recoveries of ZIKV RNA in surface water versus wastewater, and lower recoveries from a freeze thaw. We also analyzed samples collected from Salvador, Brazil during the ZIKV outbreak (2015-2016) that consisted of archived samples obtained from open sewers or environmental waters thought to be contaminated by sewage. Although we did not detect any ZIKV RNA in the archived Brazil samples, results from these persistence and recovery experiments serve to inform future wastewater monitoring efforts in open sewers, an understudied and important application of wastewater monitoring.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2023 |
---|---|
Erschienen: |
2023 |
Enthalten in: |
Zur Gesamtaufnahme - volume:241 |
---|---|
Enthalten in: |
Water research - 241(2023) vom: 01. Aug., Seite 120116 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Zhu, Kevin [VerfasserIn] |
---|
Links: |
---|
Themen: |
63231-63-0 |
---|
Anmerkungen: |
Date Completed 19.06.2023 Date Revised 12.03.2024 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.1016/j.watres.2023.120116 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM357729811 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | NLM357729811 | ||
003 | DE-627 | ||
005 | 20240312233051.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231226s2023 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1016/j.watres.2023.120116 |2 doi | |
028 | 5 | 2 | |a pubmed24n1324.xml |
035 | |a (DE-627)NLM357729811 | ||
035 | |a (NLM)37270953 | ||
035 | |a (PII)S0043-1354(23)00552-3 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Zhu, Kevin |e verfasserin |4 aut | |
245 | 1 | 0 | |a Zika virus RNA persistence and recovery in water and wastewater |b An approach for Zika virus surveillance in resource-constrained settings |
264 | 1 | |c 2023 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 19.06.2023 | ||
500 | |a Date Revised 12.03.2024 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Copyright © 2023. Published by Elsevier Ltd. | ||
520 | |a During the 2015-2016 Zika virus (ZIKV) epidemic in the Americas, serological cross-reactivity with other flaviviruses and relatively high costs of nucleic acid testing in the region hindered the capacity for widespread diagnostic testing. In such cases where individual testing is not feasible, wastewater monitoring approaches may offer a means of community-level public health surveillance. To inform such approaches, we characterized the persistence and recovery of ZIKV RNA in experiments where we spiked cultured ZIKV into surface water, wastewater, and a combination of both to examine the potential for detection in open sewers serving communities most affected by the ZIKV outbreak, such as those in Salvador, Bahia, Brazil. We used reverse transcription droplet digital PCR to quantify ZIKV RNA. In our persistence experiments, we found that the persistence of ZIKV RNA decreased with increasing temperature, significantly decreased in surface water versus wastewater, and significantly decreased when the initial concentration of virus was lowered by one order of magnitude. In our recovery experiments, we found higher percent recovery of ZIKV RNA in pellets versus supernatants from the same sample, higher recoveries in pellets using skimmed milk flocculation, lower recoveries of ZIKV RNA in surface water versus wastewater, and lower recoveries from a freeze thaw. We also analyzed samples collected from Salvador, Brazil during the ZIKV outbreak (2015-2016) that consisted of archived samples obtained from open sewers or environmental waters thought to be contaminated by sewage. Although we did not detect any ZIKV RNA in the archived Brazil samples, results from these persistence and recovery experiments serve to inform future wastewater monitoring efforts in open sewers, an understudied and important application of wastewater monitoring | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Zika virus wastewater-based epidemiology resource-constrained settings | |
650 | 7 | |a Wastewater |2 NLM | |
650 | 7 | |a RNA |2 NLM | |
650 | 7 | |a 63231-63-0 |2 NLM | |
700 | 1 | |a Hill, Cailee |e verfasserin |4 aut | |
700 | 1 | |a Muirhead, Aaron |e verfasserin |4 aut | |
700 | 1 | |a Basu, Mausumi |e verfasserin |4 aut | |
700 | 1 | |a Brown, Joe |e verfasserin |4 aut | |
700 | 1 | |a Brinton, Margo A |e verfasserin |4 aut | |
700 | 1 | |a Hayat, Matthew J |e verfasserin |4 aut | |
700 | 1 | |a Venegas-Vargas, Cristina |e verfasserin |4 aut | |
700 | 1 | |a Reis, Mitermayer G |e verfasserin |4 aut | |
700 | 1 | |a Casanovas-Massana, Arnau |e verfasserin |4 aut | |
700 | 1 | |a Meschke, J Scott |e verfasserin |4 aut | |
700 | 1 | |a Ko, Albert I |e verfasserin |4 aut | |
700 | 1 | |a Costa, Federico |e verfasserin |4 aut | |
700 | 1 | |a Stauber, Christine E |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Water research |d 1990 |g 241(2023) vom: 01. Aug., Seite 120116 |w (DE-627)NLM111404762 |x 1879-2448 |7 nnns |
773 | 1 | 8 | |g volume:241 |g year:2023 |g day:01 |g month:08 |g pages:120116 |
856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.watres.2023.120116 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 241 |j 2023 |b 01 |c 08 |h 120116 |