Human nucleolar protein 7 (NOL7) is required for early pre-rRNA accumulation and pre-18S rRNA processing
The main components of the essential cellular process of eukaryotic ribosome biogenesis are highly conserved from yeast to humans. Among these, the U3 Associated Proteins (UTPs) are a small subunit processome subcomplex that coordinate the first two steps of ribosome biogenesis in transcription and pre-18S processing. While we have identified the human counterparts of most of the yeast Utps, the homologs of yeast Utp9 and Bud21 (Utp16) have remained elusive. In this study, we find that NOL7 is the likely ortholog of Bud21. Previously described as a tumour suppressor through regulation of antiangiogenic transcripts, we now show that NOL7 is required for early pre-rRNA accumulation and pre-18S rRNA processing in human cells. These roles lead to decreased protein synthesis and induction of the nucleolar stress response upon NOL7 depletion. Beyond Bud21's nonessential role in yeast, we establish human NOL7 as an essential UTP that is necessary to maintain both early pre-rRNA levels and processing.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:20 |
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| Enthalten in: |
RNA biology - 20(2023), 1 vom: 02. Jan., Seite 257-271 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
McCool, Mason A [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 30.05.2023 Date Revised 05.06.2023 published: Print Citation Status MEDLINE |
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| doi: |
10.1080/15476286.2023.2217392 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM357489705 |
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| 245 | 1 | 0 | |a Human nucleolar protein 7 (NOL7) is required for early pre-rRNA accumulation and pre-18S rRNA processing |
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| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The main components of the essential cellular process of eukaryotic ribosome biogenesis are highly conserved from yeast to humans. Among these, the U3 Associated Proteins (UTPs) are a small subunit processome subcomplex that coordinate the first two steps of ribosome biogenesis in transcription and pre-18S processing. While we have identified the human counterparts of most of the yeast Utps, the homologs of yeast Utp9 and Bud21 (Utp16) have remained elusive. In this study, we find that NOL7 is the likely ortholog of Bud21. Previously described as a tumour suppressor through regulation of antiangiogenic transcripts, we now show that NOL7 is required for early pre-rRNA accumulation and pre-18S rRNA processing in human cells. These roles lead to decreased protein synthesis and induction of the nucleolar stress response upon NOL7 depletion. Beyond Bud21's nonessential role in yeast, we establish human NOL7 as an essential UTP that is necessary to maintain both early pre-rRNA levels and processing | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a RNA polymerase I | |
| 650 | 4 | |a Ribosome biogenesis | |
| 650 | 4 | |a nucleolus | |
| 650 | 4 | |a oncogene | |
| 650 | 4 | |a pre-ribosomal RNA processing | |
| 650 | 4 | |a rRNA | |
| 650 | 4 | |a tumour suppressor | |
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| 650 | 7 | |a RNA Precursors |2 NLM | |
| 650 | 7 | |a RNA, Ribosomal, 18S |2 NLM | |
| 650 | 7 | |a RNA, Small Nucleolar |2 NLM | |
| 650 | 7 | |a Saccharomyces cerevisiae Proteins |2 NLM | |
| 650 | 7 | |a NOL7 protein, human |2 NLM | |
| 700 | 1 | |a Bryant, Carson J |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Hannah |e verfasserin |4 aut | |
| 700 | 1 | |a Ogawa, Lisa M |e verfasserin |4 aut | |
| 700 | 1 | |a Farley-Barnes, Katherine I |e verfasserin |4 aut | |
| 700 | 1 | |a Sondalle, Samuel B |e verfasserin |4 aut | |
| 700 | 1 | |a Abriola, Laura |e verfasserin |4 aut | |
| 700 | 1 | |a Surovtseva, Yulia V |e verfasserin |4 aut | |
| 700 | 1 | |a Baserga, Susan J |e verfasserin |4 aut | |
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