Evaluation of the Autologous Genetically Enriched Leucoconcentrate on the Lumbar Spinal Cord Morpho-Functional Recovery in a Mini Pig with Thoracic Spine Contusion Injury
BACKGROUND: Pathological changes associated with spinal cord injury (SCI) can be observed distant, rostral, or caudal to the epicenter of injury. These remote areas represent important therapeutic targets for post-traumatic spinal cord repair. The present study aimed to investigate the following in relation to SCI: distant changes in the spinal cord, peripheral nerve, and muscles.
METHODS: The changes in the spinal cord, the tibial nerve, and the hind limb muscles were evaluated in control SCI animals and after intravenous infusion of autologous leucoconcentrate enriched with genes encoding neuroprotective factors (VEGF, GDNF, and NCAM), which previously demonstrated a positive effect on post-traumatic restoration.
RESULTS: Two months after thoracic contusion in the treated mini pigs, a positive remodeling of the macro- and microglial cells, expression of PSD95 and Chat in the lumbar spinal cord, and preservation of the number and morphological characteristics of the myelinated fibers in the tibial nerve were observed and were aligned with hind limb motor recovery and reduced soleus muscle atrophy.
CONCLUSION: Here, we show the positive effect of autologous genetically enriched leucoconcentrate-producing recombinant neuroprotective factors on targets distant to the primary lesion site in mini pigs with SCI. These findings open new perspectives for the therapy of SCI.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:11 |
|---|---|
| Enthalten in: |
Biomedicines - 11(2023), 5 vom: 30. Apr. |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Garifulin, Ravil [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Revised 30.05.2023 published: Electronic Citation Status PubMed-not-MEDLINE |
|---|
| doi: |
10.3390/biomedicines11051331 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM357412303 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM357412303 | ||
| 003 | DE-627 | ||
| 005 | 20231226072534.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3390/biomedicines11051331 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1191.xml |
| 035 | |a (DE-627)NLM357412303 | ||
| 035 | |a (NLM)37239001 | ||
| 035 | |a (PII)1331 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Garifulin, Ravil |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Evaluation of the Autologous Genetically Enriched Leucoconcentrate on the Lumbar Spinal Cord Morpho-Functional Recovery in a Mini Pig with Thoracic Spine Contusion Injury |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 30.05.2023 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a BACKGROUND: Pathological changes associated with spinal cord injury (SCI) can be observed distant, rostral, or caudal to the epicenter of injury. These remote areas represent important therapeutic targets for post-traumatic spinal cord repair. The present study aimed to investigate the following in relation to SCI: distant changes in the spinal cord, peripheral nerve, and muscles | ||
| 520 | |a METHODS: The changes in the spinal cord, the tibial nerve, and the hind limb muscles were evaluated in control SCI animals and after intravenous infusion of autologous leucoconcentrate enriched with genes encoding neuroprotective factors (VEGF, GDNF, and NCAM), which previously demonstrated a positive effect on post-traumatic restoration | ||
| 520 | |a RESULTS: Two months after thoracic contusion in the treated mini pigs, a positive remodeling of the macro- and microglial cells, expression of PSD95 and Chat in the lumbar spinal cord, and preservation of the number and morphological characteristics of the myelinated fibers in the tibial nerve were observed and were aligned with hind limb motor recovery and reduced soleus muscle atrophy | ||
| 520 | |a CONCLUSION: Here, we show the positive effect of autologous genetically enriched leucoconcentrate-producing recombinant neuroprotective factors on targets distant to the primary lesion site in mini pigs with SCI. These findings open new perspectives for the therapy of SCI | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a autologous blood transfusion | |
| 650 | 4 | |a genetic therapy | |
| 650 | 4 | |a genetic vectors | |
| 650 | 4 | |a glial cell line-derived neurotrophic factor | |
| 650 | 4 | |a leukocytes | |
| 650 | 4 | |a neural cell adhesion molecules | |
| 650 | 4 | |a spinal cord injuries | |
| 650 | 4 | |a spinal cord regeneration | |
| 650 | 4 | |a swine | |
| 650 | 4 | |a vascular endothelial growth factor A | |
| 700 | 1 | |a Davleeva, Maria |e verfasserin |4 aut | |
| 700 | 1 | |a Izmailov, Andrei |e verfasserin |4 aut | |
| 700 | 1 | |a Fadeev, Filip |e verfasserin |4 aut | |
| 700 | 1 | |a Markosyan, Vage |e verfasserin |4 aut | |
| 700 | 1 | |a Shevchenko, Roman |e verfasserin |4 aut | |
| 700 | 1 | |a Minyazeva, Irina |e verfasserin |4 aut | |
| 700 | 1 | |a Minekayev, Tagir |e verfasserin |4 aut | |
| 700 | 1 | |a Lavrov, Igor |e verfasserin |4 aut | |
| 700 | 1 | |a Islamov, Rustem |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biomedicines |d 2013 |g 11(2023), 5 vom: 30. Apr. |w (DE-627)NLM268899797 |x 2227-9059 |7 nnns |
| 773 | 1 | 8 | |g volume:11 |g year:2023 |g number:5 |g day:30 |g month:04 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.3390/biomedicines11051331 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 11 |j 2023 |e 5 |b 30 |c 04 | ||