Characterization of fab2 T-DNA insertion mutants in terms of fatty acid composition and plant phenotype
Fatty acid biosynthesis 2 (FAB2) is an essential enzyme responsible for the synthesis of unsaturated fatty acids in chloroplast membrane lipids found in leaves and triacylglycerols (TAG) in seeds. FAB2 functions at the junction of saturated to unsaturated fatty acid conversion in chloroplasts by converting 18:0-ACP to 18:1-ACP. In the present study, plant growth and seed phenotypes were examined in three Arabidopsis T-DNA mutants (fab2-1, fab2-2, and fab2-3). The three fab2 T-DNA mutants exhibited increased 18:0 fatty acid content in both the leaves and seeds. The degree of growth inhibition of the fab2 mutant was proportional to the increase in 18:0 and decrease in 18:3 fatty acids present in the leaves. The FAB2 mutation affected seed yield but not the seed phenotype. This result indicates that FAB2 affects the fatty acid composition of the leaf chloroplast membrane more than seed TAG. In summary, the characteristics of these three fab2 mutants provide information for studying leaf membrane lipid and seed oil biosynthesis.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2023 |
---|---|
Erschienen: |
2023 |
Enthalten in: |
Zur Gesamtaufnahme - volume:18 |
---|---|
Enthalten in: |
Plant signaling & behavior - 18(2023), 1 vom: 31. Dez., Seite 2213937 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Park, Mid-Eum [VerfasserIn] |
---|
Links: |
---|
Anmerkungen: |
Date Completed 26.05.2023 Date Revised 01.06.2023 published: Print Citation Status MEDLINE |
---|
doi: |
10.1080/15592324.2023.2213937 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM357065646 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM357065646 | ||
003 | DE-627 | ||
005 | 20231226071805.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231226s2023 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1080/15592324.2023.2213937 |2 doi | |
028 | 5 | 2 | |a pubmed24n1190.xml |
035 | |a (DE-627)NLM357065646 | ||
035 | |a (NLM)37204036 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Park, Mid-Eum |e verfasserin |4 aut | |
245 | 1 | 0 | |a Characterization of fab2 T-DNA insertion mutants in terms of fatty acid composition and plant phenotype |
264 | 1 | |c 2023 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 26.05.2023 | ||
500 | |a Date Revised 01.06.2023 | ||
500 | |a published: Print | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Fatty acid biosynthesis 2 (FAB2) is an essential enzyme responsible for the synthesis of unsaturated fatty acids in chloroplast membrane lipids found in leaves and triacylglycerols (TAG) in seeds. FAB2 functions at the junction of saturated to unsaturated fatty acid conversion in chloroplasts by converting 18:0-ACP to 18:1-ACP. In the present study, plant growth and seed phenotypes were examined in three Arabidopsis T-DNA mutants (fab2-1, fab2-2, and fab2-3). The three fab2 T-DNA mutants exhibited increased 18:0 fatty acid content in both the leaves and seeds. The degree of growth inhibition of the fab2 mutant was proportional to the increase in 18:0 and decrease in 18:3 fatty acids present in the leaves. The FAB2 mutation affected seed yield but not the seed phenotype. This result indicates that FAB2 affects the fatty acid composition of the leaf chloroplast membrane more than seed TAG. In summary, the characteristics of these three fab2 mutants provide information for studying leaf membrane lipid and seed oil biosynthesis | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 4 | |a Arabidopsis | |
650 | 4 | |a FAB2 | |
650 | 4 | |a T-DNA | |
650 | 4 | |a chloroplast | |
650 | 4 | |a fatty acid | |
650 | 4 | |a phenotype | |
650 | 7 | |a Arabidopsis Proteins |2 NLM | |
650 | 7 | |a Fatty Acids |2 NLM | |
650 | 7 | |a Fatty Acids, Unsaturated |2 NLM | |
650 | 7 | |a Membrane Lipids |2 NLM | |
650 | 7 | |a Plant Oils |2 NLM | |
650 | 7 | |a T-DNA |2 NLM | |
650 | 7 | |a stearoyl ACP desaturase, Arabidopsis |2 NLM | |
650 | 7 | |a EC 1.14.99.- |2 NLM | |
650 | 7 | |a Fatty Acid Desaturases |2 NLM | |
650 | 7 | |a EC 1.14.19.- |2 NLM | |
700 | 1 | |a Lee, Hye Ji |e verfasserin |4 aut | |
700 | 1 | |a Kim, Inyoung |e verfasserin |4 aut | |
700 | 1 | |a Kim, Hyun Uk |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Plant signaling & behavior |d 2006 |g 18(2023), 1 vom: 31. Dez., Seite 2213937 |w (DE-627)NLM189163038 |x 1559-2324 |7 nnns |
773 | 1 | 8 | |g volume:18 |g year:2023 |g number:1 |g day:31 |g month:12 |g pages:2213937 |
856 | 4 | 0 | |u http://dx.doi.org/10.1080/15592324.2023.2213937 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 18 |j 2023 |e 1 |b 31 |c 12 |h 2213937 |