Buyang huanwu decoction (BYHWD) alleviates sepsis-induced myocardial injury by suppressing local immune cell infiltration and skewing M2-macrophage polarization
AJTR Copyright © 2023..
OBJECTIVE: To investigate the therapeutic effect of Buyang huanwu decoction (BYHWD) on sepsis-induced myocardial injury (SIMI) and explore the mechanism by which BYHWD mitigates SIMI.
METHODS: The Lipopolysaccharide (LPS)-induced SIMI mouse model was established to detect the effect of BYHWD-low (1 mg/kg), BYHWD-middle (5 mg/kg), and BYWHD-high (20 mg/kg) on SIMI. The survival of these BYHWD-treated septic mice was investigated. The histology of myocardial tissues was determined by hematoxylin and eosin (H&E) staining. The apoptotic index and inflamed microenvironment of myocardial tissues were assessed by immunofluorescent staining (IF) and flow cytometry analysis. Liquid chromatography-mass spectrometry (LC-MS/MS) was employed to determine the key chemical components in the serum of BYHWD-loaded septic mice. Immunoblotting assay was utilized to detect NF-κB and TGF-β signaling activity, and M1/M2-macrophage markers using RAW264.7 cells.
RESULTS: The high dosage of BYHWD (BYHWD-high, 20 mg/Kg) significantly attenuated SIMI and improved the survival of septic mice. The BYHWD-high solution markedly reduced myocardial cell apoptosis and mitigated the inflamed microenvironment by suppressing CD45+ immune cell infiltration. Importantly, BYHWD decreased macrophage accumulation and promoted an M2-macrophage polarization. Paeoniflorin (PF) and calycosin-7-O-β-glucoside (CBG) were identified as the key molecules in BYWHD with therapeutic effect. PF (10 μM) and CBG (1 μM) inhibited NF-κB signaling, meanwhile they upregulated the TGF-β pathway, thereby facilitating an M2-macrophage phenotypic transition in RAW264.7 cells.
CONCLUSIONS: BYHWD, with two effective components PF and CBG, can mitigate SIMI by suppressing the inflamed myocardial microenvironment and skewing an immunosuppressive M2-macrophage phenotype.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:15 |
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| Enthalten in: |
American journal of translational research - 15(2023), 4 vom: 16., Seite 2389-2406 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Xiao, Lingling [VerfasserIn] |
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| Themen: |
Buyang huanwu decoction (BYHWD) |
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| Anmerkungen: |
Date Revised 19.05.2023 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM356957756 |
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| LEADER | 01000naa a22002652 4500 | ||
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| 041 | |a eng | ||
| 100 | 1 | |a Xiao, Lingling |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Buyang huanwu decoction (BYHWD) alleviates sepsis-induced myocardial injury by suppressing local immune cell infiltration and skewing M2-macrophage polarization |
| 264 | 1 | |c 2023 | |
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| 500 | |a Date Revised 19.05.2023 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a AJTR Copyright © 2023. | ||
| 520 | |a OBJECTIVE: To investigate the therapeutic effect of Buyang huanwu decoction (BYHWD) on sepsis-induced myocardial injury (SIMI) and explore the mechanism by which BYHWD mitigates SIMI | ||
| 520 | |a METHODS: The Lipopolysaccharide (LPS)-induced SIMI mouse model was established to detect the effect of BYHWD-low (1 mg/kg), BYHWD-middle (5 mg/kg), and BYWHD-high (20 mg/kg) on SIMI. The survival of these BYHWD-treated septic mice was investigated. The histology of myocardial tissues was determined by hematoxylin and eosin (H&E) staining. The apoptotic index and inflamed microenvironment of myocardial tissues were assessed by immunofluorescent staining (IF) and flow cytometry analysis. Liquid chromatography-mass spectrometry (LC-MS/MS) was employed to determine the key chemical components in the serum of BYHWD-loaded septic mice. Immunoblotting assay was utilized to detect NF-κB and TGF-β signaling activity, and M1/M2-macrophage markers using RAW264.7 cells | ||
| 520 | |a RESULTS: The high dosage of BYHWD (BYHWD-high, 20 mg/Kg) significantly attenuated SIMI and improved the survival of septic mice. The BYHWD-high solution markedly reduced myocardial cell apoptosis and mitigated the inflamed microenvironment by suppressing CD45+ immune cell infiltration. Importantly, BYHWD decreased macrophage accumulation and promoted an M2-macrophage polarization. Paeoniflorin (PF) and calycosin-7-O-β-glucoside (CBG) were identified as the key molecules in BYWHD with therapeutic effect. PF (10 μM) and CBG (1 μM) inhibited NF-κB signaling, meanwhile they upregulated the TGF-β pathway, thereby facilitating an M2-macrophage phenotypic transition in RAW264.7 cells | ||
| 520 | |a CONCLUSIONS: BYHWD, with two effective components PF and CBG, can mitigate SIMI by suppressing the inflamed myocardial microenvironment and skewing an immunosuppressive M2-macrophage phenotype | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Buyang huanwu decoction (BYHWD) | |
| 650 | 4 | |a M2-macrophage | |
| 650 | 4 | |a NF-κB signaling | |
| 650 | 4 | |a TGF-β signaling | |
| 650 | 4 | |a inflamed microenvironment | |
| 650 | 4 | |a sepsis-induced myocardial injury (SIMI) | |
| 700 | 1 | |a Xi, Xixiang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Meng |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Lifen |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Kai |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Zhenyu |e verfasserin |4 aut | |
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