A multi-center, prospective cohort study of whole blood gene expression in the tuberculosis-diabetes interaction
© 2023. The Author(s)..
Diabetes mellitus (DM) increases tuberculosis (TB) severity. We compared blood gene expression in adults with pulmonary TB, with or without diabetes mellitus (DM) from sites in Brazil and India. RNA sequencing (RNAseq) performed at baseline and during TB treatment. Publicly available baseline RNAseq data from South Africa and Romania reported by the TANDEM Consortium were also analyzed. Across the sites, differentially expressed genes varied for each condition (DM, TB, and TBDM) and no pattern classified any one group across all sites. A concise signature of TB disease was identified but this was expressed equally in TB and TBDM. Pathway enrichment analysis failed to distinguish TB from TBDM, although there was a trend for greater neutrophil and innate immune pathway activation in TBDM participants. Pathways associated with insulin resistance, metabolic dysfunction, diabetic complications, and chromosomal instability were positively correlated with glycohemoglobin. The immune response to pulmonary TB as reflected by whole blood gene expression is substantially similar with or without comorbid DM. Gene expression pathways associated with the microvascular and macrovascular complications of DM are upregulated during TB, supporting a syndemic interaction between these coprevalent diseases.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
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| Enthalten in: |
Scientific reports - 13(2023), 1 vom: 12. Mai, Seite 7769 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Queiroz, Artur T L [VerfasserIn] |
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| Links: |
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| Themen: |
Journal Article |
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| Anmerkungen: |
Date Completed 15.05.2023 Date Revised 11.11.2023 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s41598-023-34847-9 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM356762300 |
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| 100 | 1 | |a Queiroz, Artur T L |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A multi-center, prospective cohort study of whole blood gene expression in the tuberculosis-diabetes interaction |
| 264 | 1 | |c 2023 | |
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| 500 | |a Date Completed 15.05.2023 | ||
| 500 | |a Date Revised 11.11.2023 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2023. The Author(s). | ||
| 520 | |a Diabetes mellitus (DM) increases tuberculosis (TB) severity. We compared blood gene expression in adults with pulmonary TB, with or without diabetes mellitus (DM) from sites in Brazil and India. RNA sequencing (RNAseq) performed at baseline and during TB treatment. Publicly available baseline RNAseq data from South Africa and Romania reported by the TANDEM Consortium were also analyzed. Across the sites, differentially expressed genes varied for each condition (DM, TB, and TBDM) and no pattern classified any one group across all sites. A concise signature of TB disease was identified but this was expressed equally in TB and TBDM. Pathway enrichment analysis failed to distinguish TB from TBDM, although there was a trend for greater neutrophil and innate immune pathway activation in TBDM participants. Pathways associated with insulin resistance, metabolic dysfunction, diabetic complications, and chromosomal instability were positively correlated with glycohemoglobin. The immune response to pulmonary TB as reflected by whole blood gene expression is substantially similar with or without comorbid DM. Gene expression pathways associated with the microvascular and macrovascular complications of DM are upregulated during TB, supporting a syndemic interaction between these coprevalent diseases | ||
| 650 | 4 | |a Multicenter Study | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 700 | 1 | |a Vinhaes, Caian L |e verfasserin |4 aut | |
| 700 | 1 | |a Fukutani, Eduardo R |e verfasserin |4 aut | |
| 700 | 1 | |a Gupte, Akshay N |e verfasserin |4 aut | |
| 700 | 1 | |a Kumar, Nathella Pavan |e verfasserin |4 aut | |
| 700 | 1 | |a Fukutani, Kiyoshi F |e verfasserin |4 aut | |
| 700 | 1 | |a Arriaga, María B |e verfasserin |4 aut | |
| 700 | 1 | |a Sterling, Timothy R |e verfasserin |4 aut | |
| 700 | 1 | |a Babu, Subash |e verfasserin |4 aut | |
| 700 | 1 | |a Gaikwad, Sanjay |e verfasserin |4 aut | |
| 700 | 1 | |a Karyakarte, Rajesh |e verfasserin |4 aut | |
| 700 | 1 | |a Mave, Vidya |e verfasserin |4 aut | |
| 700 | 1 | |a Paradhkar, Mandar |e verfasserin |4 aut | |
| 700 | 1 | |a Viswanathan, Vijay |e verfasserin |4 aut | |
| 700 | 1 | |a Gupta, Amita |e verfasserin |4 aut | |
| 700 | 1 | |a Andrade, Bruno B |e verfasserin |4 aut | |
| 700 | 1 | |a Kornfeld, Hardy |e verfasserin |4 aut | |
| 700 | 0 | |a RePORT Brazil |e verfasserin |4 aut | |
| 700 | 0 | |a RePORT India Consortia |e verfasserin |4 aut | |
| 700 | 1 | |a Andrade, Alice M S |e investigator |4 oth | |
| 700 | 1 | |a Figueiredo, Marina C |e investigator |4 oth | |
| 700 | 1 | |a Nascimento, Vanessa |e investigator |4 oth | |
| 700 | 1 | |a Cubillos-Angulo, Juan Manuel |e investigator |4 oth | |
| 700 | 1 | |a Malta-Santos, Hayna |e investigator |4 oth | |
| 700 | 1 | |a Rebouças-Silva, Jéssica |e investigator |4 oth | |
| 700 | 1 | |a Gomes-Silva, Adriano |e investigator |4 oth | |
| 700 | 1 | |a Santos, Saulo R N |e investigator |4 oth | |
| 700 | 1 | |a Ramos, André |e investigator |4 oth | |
| 700 | 1 | |a Brito, Pedro |e investigator |4 oth | |
| 700 | 1 | |a Schmaltz, Carolina A S |e investigator |4 oth | |
| 700 | 1 | |a Costa, Alysson G |e investigator |4 oth | |
| 700 | 1 | |a Garcia, Leandro Sousa |e investigator |4 oth | |
| 700 | 1 | |a de Sousa Carvalho, Brenda K |e investigator |4 oth | |
| 700 | 1 | |a de Loiola, Bruna P |e investigator |4 oth | |
| 700 | 1 | |a Ignácio, Francine P |e investigator |4 oth | |
| 700 | 1 | |a Lourenço, Maria C |e investigator |4 oth | |
| 700 | 1 | |a Silva, Elisangela C |e investigator |4 oth | |
| 700 | 1 | |a Mello, Mayla |e investigator |4 oth | |
| 700 | 1 | |a Souza, Alexandra B |e investigator |4 oth | |
| 700 | 1 | |a Rocha, Michael S |e investigator |4 oth | |
| 700 | 1 | |a Benjamin, Aline |e investigator |4 oth | |
| 700 | 1 | |a Moreira, Adriana S R |e investigator |4 oth | |
| 700 | 1 | |a de Oliveira, Jamile G |e investigator |4 oth | |
| 700 | 1 | |a Cavalcante, Solange |e investigator |4 oth | |
| 700 | 1 | |a Durovni, Betina |e investigator |4 oth | |
| 700 | 1 | |a Cordeiro-Santos, Marcelo |e investigator |4 oth | |
| 700 | 1 | |a Kristki, Afrânio L |e investigator |4 oth | |
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