Hemagglutinin virus-like particles incorporated with membrane-bound cytokine adjuvants provide protection against homologous and heterologous influenza virus challenge in aged mice
© 2023. The Author(s)..
BACKGROUND: Current influenza vaccines deliver satisfactory results in young people but are less effective in the elderly. Development of vaccines for an ever-increasing aging population has been an arduous challenge due to immunosenescence that impairs the immune response in the aged, both quantitatively and qualitatively.
RESULTS: To potentially enhance vaccine efficacy in the elderly, we investigated the immunogenicity and cross-protection of influenza hemagglutinin virus-like particles (HA-VLP) incorporated with glycosylphosphatidylinositol (GPI)-anchored cytokine-adjuvants (GPI-GM-CSF and GPI-IL-12) via protein transfer in aged mice. Lung viral replication against homologous and heterologous influenza viruses was significantly reduced in aged mice after vaccination with cytokine incorporated VLPs (HA-VLP-Cyt) in comparison to HA-VLP alone. Enhanced IFN-γ+CD4+ and IFN-γ+CD8+ T cell responses were also observed in aged mice immunized with HA-VLP-Cyt when compared to HA-VLP alone.
CONCLUSIONS: Cytokine-adjuvanted influenza HA-VLP vaccine induced enhanced protective response against homologous influenza A virus infection in aged mice. Influenza HA-VLP vaccine with GPI-cytokines also induced enhanced T cell responses correlating with better protection against heterologous infection in the absence of neutralizing antibodies. The results suggest that a vaccination strategy using cytokine-adjuvanted influenza HA-VLPs could be used to enhance protection against influenza A virus in the elderly.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2023 |
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Erschienen: |
2023 |
Enthalten in: |
Zur Gesamtaufnahme - volume:20 |
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Enthalten in: |
Immunity & ageing : I & A - 20(2023), 1 vom: 11. Mai, Seite 20 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Park, Bo Ryoung [VerfasserIn] |
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Links: |
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Themen: |
Aged mice |
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Anmerkungen: |
Date Revised 18.07.2023 published: Electronic Citation Status PubMed-not-MEDLINE |
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doi: |
10.1186/s12979-023-00344-w |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM356731057 |
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520 | |a BACKGROUND: Current influenza vaccines deliver satisfactory results in young people but are less effective in the elderly. Development of vaccines for an ever-increasing aging population has been an arduous challenge due to immunosenescence that impairs the immune response in the aged, both quantitatively and qualitatively | ||
520 | |a RESULTS: To potentially enhance vaccine efficacy in the elderly, we investigated the immunogenicity and cross-protection of influenza hemagglutinin virus-like particles (HA-VLP) incorporated with glycosylphosphatidylinositol (GPI)-anchored cytokine-adjuvants (GPI-GM-CSF and GPI-IL-12) via protein transfer in aged mice. Lung viral replication against homologous and heterologous influenza viruses was significantly reduced in aged mice after vaccination with cytokine incorporated VLPs (HA-VLP-Cyt) in comparison to HA-VLP alone. Enhanced IFN-γ+CD4+ and IFN-γ+CD8+ T cell responses were also observed in aged mice immunized with HA-VLP-Cyt when compared to HA-VLP alone | ||
520 | |a CONCLUSIONS: Cytokine-adjuvanted influenza HA-VLP vaccine induced enhanced protective response against homologous influenza A virus infection in aged mice. Influenza HA-VLP vaccine with GPI-cytokines also induced enhanced T cell responses correlating with better protection against heterologous infection in the absence of neutralizing antibodies. The results suggest that a vaccination strategy using cytokine-adjuvanted influenza HA-VLPs could be used to enhance protection against influenza A virus in the elderly | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Aged mice | |
650 | 4 | |a Cross protection | |
650 | 4 | |a Cytokine adjuvants | |
650 | 4 | |a GPI-GM-CSF | |
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650 | 4 | |a Hemagglutinin (HA) | |
650 | 4 | |a Influenza | |
650 | 4 | |a T cells | |
650 | 4 | |a Virus-like particles (HA-VLPs) | |
700 | 1 | |a Bommireddy, Ramireddy |e verfasserin |4 aut | |
700 | 1 | |a Chung, David Hyunjung |e verfasserin |4 aut | |
700 | 1 | |a Kim, Ki-Hye |e verfasserin |4 aut | |
700 | 1 | |a Subbiah, Jeeva |e verfasserin |4 aut | |
700 | 1 | |a Jung, Yu-Jin |e verfasserin |4 aut | |
700 | 1 | |a Bhatnagar, Noopur |e verfasserin |4 aut | |
700 | 1 | |a Pack, Christopher D |e verfasserin |4 aut | |
700 | 1 | |a Ramachandiran, Sampath |e verfasserin |4 aut | |
700 | 1 | |a Reddy, Shaker J C |e verfasserin |4 aut | |
700 | 1 | |a Selvaraj, Periasamy |e verfasserin |4 aut | |
700 | 1 | |a Kang, Sang-Moo |e verfasserin |4 aut | |
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