A simple, dual direct expression plasmid system in prokaryotic and mammalian cells
© The Author(s) 2023. Published by Oxford University Press on behalf of National Academy of Sciences..
We introduce a simple, dual direct cloning plasmid system (pgMAX-II) for gene expression analysis in both prokaryotic (Escherichia coli) and mammalian cells. This system, which uses a prokaryotic expression unit adapted from the pgMAX system and a mammalian promoter, is effective for subcloning using the DNA topoisomerase II toxin CcdB. Given that molecular biological cloning systems broadly rely on E. coli for rapid growth, the proposed concept may have wide applicability beyond mammalian cells.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2 |
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| Enthalten in: |
PNAS nexus - 2(2023), 5 vom: 04. Mai, Seite pgad139 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Murakami, Manabu [VerfasserIn] |
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| Links: |
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| Themen: |
DNA recombination |
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| Anmerkungen: |
Date Revised 14.05.2023 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1093/pnasnexus/pgad139 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM356715515 |
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| 520 | |a © The Author(s) 2023. Published by Oxford University Press on behalf of National Academy of Sciences. | ||
| 520 | |a We introduce a simple, dual direct cloning plasmid system (pgMAX-II) for gene expression analysis in both prokaryotic (Escherichia coli) and mammalian cells. This system, which uses a prokaryotic expression unit adapted from the pgMAX system and a mammalian promoter, is effective for subcloning using the DNA topoisomerase II toxin CcdB. Given that molecular biological cloning systems broadly rely on E. coli for rapid growth, the proposed concept may have wide applicability beyond mammalian cells | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a mammalian expression vector | |
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| 700 | 1 | |a Miyoshi, Ichiro |e verfasserin |4 aut | |
| 700 | 1 | |a Itagaki, Shirou |e verfasserin |4 aut | |
| 700 | 1 | |a Niwa, Yasutaka |e verfasserin |4 aut | |
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