Developmental effects of TCIPP and TnBP on zebrafish (Danio rerio) embryos
Objective: To investigate the toxicity of tris (2-chloropropyl) phosphate (TCIPP) and tributyl phosphate (TnBP) on the growth and development of zebrafish embryos, as well as to explore the underlying mechanisms at the transcriptional level. Methods: With zebrafish as a model, two hpf zebrafish embryos were exposed to TCIPP and TnBP (0.1, 1, 10, 100, 500, and 1 000 μmol/L) using the semi-static method, and their rates of lethality and hatchability were determined. The transcriptome changes of 120 hpf juvenile zebrafish exposed to environmentally relevant concentrations of 0.1 and 1 μmol/L were measured. Results: The 50% lethal concentrations (LC50) of TCIPP and TnBP for zebrafish embryos were 155.30 and 27.62 μmol/L (96 hpf), 156.5 and 26.05 μmol/L (120 hpf), respectively. The 72 hpf hatching rates of TCIPP (100 μmol/L) and TnBP (10 μmol/L) were (23.33±7.72)% and (91.67±2.97)%, which were significantly decreased compared with the control group (P<0.05). Transcriptome analysis showed that TnBP had more differential genes (DEGs) than TCIPP, with a dose-response relationship. These DEGs were enriched in 32 pathways in total, including those involved in oxidative stress, energy metabolism, lipid metabolism, and nuclear receptor-related pathways, using the IPA pathway analysis. Among them, three enriched pathways overlapped between TCIPP and TnBP, including TR/RXR activation and CAR/RXR activation. Additionally, DEGs were also mapped onto pathways of LXR/RXR activation and oxidative stress for TnBP exposure only. Conclusion: Both TCIPP and TnBP have growth and developmental toxicities in zebrafish embryos, with distinct biomolecular mechanisms, and TnBP has a stronger effect than TCIPP.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2023 |
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Erschienen: |
2023 |
Enthalten in: |
Zur Gesamtaufnahme - volume:57 |
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Enthalten in: |
Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine - 57(2023), 5 vom: 06. Mai, Seite 693-700 |
Sprache: |
Chinesisch |
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Beteiligte Personen: |
Zhang, S Y [VerfasserIn] |
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Links: |
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Themen: |
95UAS8YAF5 |
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Anmerkungen: |
Date Completed 12.05.2023 Date Revised 12.05.2023 published: Print Citation Status MEDLINE |
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doi: |
10.3760/cma.j.cn112150-20230218-00124 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM356687163 |
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520 | |a Objective: To investigate the toxicity of tris (2-chloropropyl) phosphate (TCIPP) and tributyl phosphate (TnBP) on the growth and development of zebrafish embryos, as well as to explore the underlying mechanisms at the transcriptional level. Methods: With zebrafish as a model, two hpf zebrafish embryos were exposed to TCIPP and TnBP (0.1, 1, 10, 100, 500, and 1 000 μmol/L) using the semi-static method, and their rates of lethality and hatchability were determined. The transcriptome changes of 120 hpf juvenile zebrafish exposed to environmentally relevant concentrations of 0.1 and 1 μmol/L were measured. Results: The 50% lethal concentrations (LC50) of TCIPP and TnBP for zebrafish embryos were 155.30 and 27.62 μmol/L (96 hpf), 156.5 and 26.05 μmol/L (120 hpf), respectively. The 72 hpf hatching rates of TCIPP (100 μmol/L) and TnBP (10 μmol/L) were (23.33±7.72)% and (91.67±2.97)%, which were significantly decreased compared with the control group (P<0.05). Transcriptome analysis showed that TnBP had more differential genes (DEGs) than TCIPP, with a dose-response relationship. These DEGs were enriched in 32 pathways in total, including those involved in oxidative stress, energy metabolism, lipid metabolism, and nuclear receptor-related pathways, using the IPA pathway analysis. Among them, three enriched pathways overlapped between TCIPP and TnBP, including TR/RXR activation and CAR/RXR activation. Additionally, DEGs were also mapped onto pathways of LXR/RXR activation and oxidative stress for TnBP exposure only. Conclusion: Both TCIPP and TnBP have growth and developmental toxicities in zebrafish embryos, with distinct biomolecular mechanisms, and TnBP has a stronger effect than TCIPP | ||
650 | 4 | |a English Abstract | |
650 | 4 | |a Journal Article | |
650 | 7 | |a tributyl phosphate |2 NLM | |
650 | 7 | |a 95UAS8YAF5 |2 NLM | |
650 | 7 | |a Water Pollutants, Chemical |2 NLM | |
700 | 1 | |a Zhang, S P |e verfasserin |4 aut | |
700 | 1 | |a Shao, Z J |e verfasserin |4 aut | |
700 | 1 | |a Fu, Y Z |e verfasserin |4 aut | |
700 | 1 | |a Gu, W |e verfasserin |4 aut | |
700 | 1 | |a Zhi, H |e verfasserin |4 aut | |
700 | 1 | |a Kong, J |e verfasserin |4 aut | |
700 | 1 | |a Deng, F C |e verfasserin |4 aut | |
700 | 1 | |a Yan, W Y |e verfasserin |4 aut | |
700 | 1 | |a Liu, J |e verfasserin |4 aut | |
700 | 1 | |a Wang, C |e verfasserin |4 aut | |
700 | 1 | |a Tang, S |e verfasserin |4 aut | |
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