Phage display sequencing reveals that genetic, environmental, and intrinsic factors influence variation of human antibody epitope repertoire
Copyright © 2023 Elsevier Inc. All rights reserved..
Phage-displayed immunoprecipitation sequencing (PhIP-seq) has enabled high-throughput profiling of human antibody repertoires. However, a comprehensive overview of environmental and genetic determinants shaping human adaptive immunity is lacking. In this study, we investigated the effects of genetic, environmental, and intrinsic factors on the variation in human antibody repertoires. We characterized serological antibody repertoires against 344,000 peptides using PhIP-seq libraries from a wide range of microbial and environmental antigens in 1,443 participants from a population cohort. We detected individual-specificity, temporal consistency, and co-housing similarities in antibody repertoires. Genetic analyses showed the involvement of the HLA, IGHV, and FUT2 gene regions in antibody-bound peptide reactivity. Furthermore, we uncovered associations between phenotypic factors (including age, cell counts, sex, smoking behavior, and allergies, among others) and particular antibody-bound peptides. Our results indicate that human antibody epitope repertoires are shaped by both genetics and environmental exposures and highlight specific signatures of distinct phenotypes and genotypes.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:56 |
|---|---|
| Enthalten in: |
Immunity - 56(2023), 6 vom: 13. Juni, Seite 1376-1392.e8 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Andreu-Sánchez, Sergio [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Antibodies |
|---|
| Anmerkungen: |
Date Completed 05.09.2023 Date Revised 05.09.2023 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.immuni.2023.04.003 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM356669262 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM356669262 | ||
| 003 | DE-627 | ||
| 005 | 20231226070941.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.immuni.2023.04.003 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1188.xml |
| 035 | |a (DE-627)NLM356669262 | ||
| 035 | |a (NLM)37164013 | ||
| 035 | |a (PII)S1074-7613(23)00171-1 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Andreu-Sánchez, Sergio |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Phage display sequencing reveals that genetic, environmental, and intrinsic factors influence variation of human antibody epitope repertoire |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 05.09.2023 | ||
| 500 | |a Date Revised 05.09.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023 Elsevier Inc. All rights reserved. | ||
| 520 | |a Phage-displayed immunoprecipitation sequencing (PhIP-seq) has enabled high-throughput profiling of human antibody repertoires. However, a comprehensive overview of environmental and genetic determinants shaping human adaptive immunity is lacking. In this study, we investigated the effects of genetic, environmental, and intrinsic factors on the variation in human antibody repertoires. We characterized serological antibody repertoires against 344,000 peptides using PhIP-seq libraries from a wide range of microbial and environmental antigens in 1,443 participants from a population cohort. We detected individual-specificity, temporal consistency, and co-housing similarities in antibody repertoires. Genetic analyses showed the involvement of the HLA, IGHV, and FUT2 gene regions in antibody-bound peptide reactivity. Furthermore, we uncovered associations between phenotypic factors (including age, cell counts, sex, smoking behavior, and allergies, among others) and particular antibody-bound peptides. Our results indicate that human antibody epitope repertoires are shaped by both genetics and environmental exposures and highlight specific signatures of distinct phenotypes and genotypes | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a PhIP-seq | |
| 650 | 4 | |a antibody repertoire | |
| 650 | 4 | |a environment | |
| 650 | 4 | |a genetics | |
| 650 | 4 | |a lifestyle | |
| 650 | 7 | |a Antibodies |2 NLM | |
| 650 | 7 | |a Antigens |2 NLM | |
| 650 | 7 | |a Epitopes |2 NLM | |
| 650 | 7 | |a Peptides |2 NLM | |
| 700 | 1 | |a Bourgonje, Arno R |e verfasserin |4 aut | |
| 700 | 1 | |a Vogl, Thomas |e verfasserin |4 aut | |
| 700 | 1 | |a Kurilshikov, Alexander |e verfasserin |4 aut | |
| 700 | 1 | |a Leviatan, Sigal |e verfasserin |4 aut | |
| 700 | 1 | |a Ruiz-Moreno, Angel J |e verfasserin |4 aut | |
| 700 | 1 | |a Hu, Shixian |e verfasserin |4 aut | |
| 700 | 1 | |a Sinha, Trishla |e verfasserin |4 aut | |
| 700 | 1 | |a Vich Vila, Arnau |e verfasserin |4 aut | |
| 700 | 1 | |a Klompus, Shelley |e verfasserin |4 aut | |
| 700 | 1 | |a Kalka, Iris N |e verfasserin |4 aut | |
| 700 | 1 | |a de Leeuw, Karina |e verfasserin |4 aut | |
| 700 | 1 | |a Arends, Suzanne |e verfasserin |4 aut | |
| 700 | 1 | |a Jonkers, Iris |e verfasserin |4 aut | |
| 700 | 1 | |a Withoff, Sebo |e verfasserin |4 aut | |
| 700 | 0 | |a Lifelines Cohort Study |e verfasserin |4 aut | |
| 700 | 1 | |a Brouwer, Elisabeth |e verfasserin |4 aut | |
| 700 | 1 | |a Weinberger, Adina |e verfasserin |4 aut | |
| 700 | 1 | |a Wijmenga, Cisca |e verfasserin |4 aut | |
| 700 | 1 | |a Segal, Eran |e verfasserin |4 aut | |
| 700 | 1 | |a Weersma, Rinse K |e verfasserin |4 aut | |
| 700 | 1 | |a Fu, Jingyuan |e verfasserin |4 aut | |
| 700 | 1 | |a Zhernakova, Alexandra |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Immunity |d 1994 |g 56(2023), 6 vom: 13. Juni, Seite 1376-1392.e8 |w (DE-627)NLM075097818 |x 1097-4180 |7 nnns |
| 773 | 1 | 8 | |g volume:56 |g year:2023 |g number:6 |g day:13 |g month:06 |g pages:1376-1392.e8 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.immuni.2023.04.003 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 56 |j 2023 |e 6 |b 13 |c 06 |h 1376-1392.e8 | ||