A single-cell view on host immune transcriptional response to in vivo BCG-induced trained immunity
Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved..
Bacillus Calmette-Guérin (BCG) vaccination is a prototype model for the study of trained immunity (TI) in humans, and results in a more effective response of innate immune cells upon stimulation with heterologous stimuli. Here, we investigate the heterogeneity of TI induction by single-cell RNA sequencing of immune cells collected from 156 samples. We observe that both monocytes and CD8+ T cells show heterologous transcriptional responses to lipopolysaccharide, with an active crosstalk between these two cell types. Furthermore, the interferon-γ pathway is crucial in BCG-induced TI, and it is upregulated in functional high responders. Data-driven analyses and functional experiments reveal STAT1 to be one of the important transcription factors for TI shared in all identified monocyte subpopulations. Finally, we report the role of type I interferon-related and neutrophil-related TI transcriptional programs in patients with sepsis. These findings provide comprehensive insights into the importance of monocyte heterogeneity during TI in humans.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:42 |
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| Enthalten in: |
Cell reports - 42(2023), 5 vom: 30. Mai, Seite 112487 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Li, Wenchao [VerfasserIn] |
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| Links: |
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| Themen: |
82115-62-6 |
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| Anmerkungen: |
Date Completed 05.06.2023 Date Revised 08.06.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.celrep.2023.112487 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM356584631 |
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| 245 | 1 | 2 | |a A single-cell view on host immune transcriptional response to in vivo BCG-induced trained immunity |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a Bacillus Calmette-Guérin (BCG) vaccination is a prototype model for the study of trained immunity (TI) in humans, and results in a more effective response of innate immune cells upon stimulation with heterologous stimuli. Here, we investigate the heterogeneity of TI induction by single-cell RNA sequencing of immune cells collected from 156 samples. We observe that both monocytes and CD8+ T cells show heterologous transcriptional responses to lipopolysaccharide, with an active crosstalk between these two cell types. Furthermore, the interferon-γ pathway is crucial in BCG-induced TI, and it is upregulated in functional high responders. Data-driven analyses and functional experiments reveal STAT1 to be one of the important transcription factors for TI shared in all identified monocyte subpopulations. Finally, we report the role of type I interferon-related and neutrophil-related TI transcriptional programs in patients with sepsis. These findings provide comprehensive insights into the importance of monocyte heterogeneity during TI in humans | ||
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| 700 | 1 | |a Moorlag, Simone J C F M |e verfasserin |4 aut | |
| 700 | 1 | |a Koeken, Valerie A C M |e verfasserin |4 aut | |
| 700 | 1 | |a Röring, Rutger J |e verfasserin |4 aut | |
| 700 | 1 | |a de Bree, L Charlotte J |e verfasserin |4 aut | |
| 700 | 1 | |a Mourits, Vera P |e verfasserin |4 aut | |
| 700 | 1 | |a Gupta, Manoj K |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Bowen |e verfasserin |4 aut | |
| 700 | 1 | |a Fu, Jianbo |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Zhenhua |e verfasserin |4 aut | |
| 700 | 1 | |a Grondman, Inge |e verfasserin |4 aut | |
| 700 | 1 | |a van Meijgaarden, Krista E |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Liang |e verfasserin |4 aut | |
| 700 | 1 | |a Alaswad, Ahmed |e verfasserin |4 aut | |
| 700 | 1 | |a Joosten, Leo A B |e verfasserin |4 aut | |
| 700 | 1 | |a van Crevel, Reinout |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Cheng-Jian |e verfasserin |4 aut | |
| 700 | 1 | |a Netea, Mihai G |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Yang |e verfasserin |4 aut | |
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