Short-term exposition to acute cadmium toxicity induces the loss of root gravitropic stimuli perception through PIN2-mediated auxin redistribution in Arabidopsis thaliana (L.) Heynh
Copyright © 2023 Elsevier B.V. All rights reserved..
Cadmium (Cd), one of the most widespread and water-soluble polluting heavy metals, has been widely studied on plants, even if the mechanisms underlying its phytotoxicity remain elusive. Indeed, most experiments are performed using extensive exposure time to the toxicants, not observing the primary targets affected. The present work studied Cd effects on Arabidopsis thaliana (L.) Heynh's root apical meristem (RAM) exposed for short periods (24 h and 48 h) to acute phytotoxic concentrations (100 and 150 µM). The effects were studied through integrated morpho-histological, molecular, pharmacological and metabolomic analyses, highlighting that Cd inhibited primary root elongation by affecting the meristem zone via altering cell expansion. Moreover, Cd altered Auxin accumulation in RAM and affected PINs polar transporters, particularly PIN2. In addition, we observed that high Cd concentration induced accumulation of reactive oxygen species (ROS) in roots, which resulted in an altered organization of cortical microtubules and the starch and sucrose metabolism, altering the statolith formation and, consequently, the gravitropic root response. Our results demonstrated that short Cd exposition (24 h) affected cell expansion preferentially, altering auxin distribution and inducing ROS accumulation, which resulted in an alteration of gravitropic response and microtubules orientation pattern.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:332 |
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| Enthalten in: |
Plant science : an international journal of experimental plant biology - 332(2023) vom: 15. Juli, Seite 111726 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Araniti, Fabrizio [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 29.05.2023 Date Revised 29.05.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.plantsci.2023.111726 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 100 | 1 | |a Araniti, Fabrizio |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Short-term exposition to acute cadmium toxicity induces the loss of root gravitropic stimuli perception through PIN2-mediated auxin redistribution in Arabidopsis thaliana (L.) Heynh |
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| 520 | |a Copyright © 2023 Elsevier B.V. All rights reserved. | ||
| 520 | |a Cadmium (Cd), one of the most widespread and water-soluble polluting heavy metals, has been widely studied on plants, even if the mechanisms underlying its phytotoxicity remain elusive. Indeed, most experiments are performed using extensive exposure time to the toxicants, not observing the primary targets affected. The present work studied Cd effects on Arabidopsis thaliana (L.) Heynh's root apical meristem (RAM) exposed for short periods (24 h and 48 h) to acute phytotoxic concentrations (100 and 150 µM). The effects were studied through integrated morpho-histological, molecular, pharmacological and metabolomic analyses, highlighting that Cd inhibited primary root elongation by affecting the meristem zone via altering cell expansion. Moreover, Cd altered Auxin accumulation in RAM and affected PINs polar transporters, particularly PIN2. In addition, we observed that high Cd concentration induced accumulation of reactive oxygen species (ROS) in roots, which resulted in an altered organization of cortical microtubules and the starch and sucrose metabolism, altering the statolith formation and, consequently, the gravitropic root response. Our results demonstrated that short Cd exposition (24 h) affected cell expansion preferentially, altering auxin distribution and inducing ROS accumulation, which resulted in an alteration of gravitropic response and microtubules orientation pattern | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Auxin transport | |
| 650 | 4 | |a Heavy metals | |
| 650 | 4 | |a Metabolomics | |
| 650 | 4 | |a Oxidative stress | |
| 650 | 4 | |a Root gravitropism | |
| 650 | 4 | |a Starch and sucrose metabolism | |
| 650 | 7 | |a Cadmium |2 NLM | |
| 650 | 7 | |a 00BH33GNGH |2 NLM | |
| 650 | 7 | |a Arabidopsis Proteins |2 NLM | |
| 650 | 7 | |a Indoleacetic Acids |2 NLM | |
| 650 | 7 | |a Reactive Oxygen Species |2 NLM | |
| 650 | 7 | |a PIN2 protein, Arabidopsis |2 NLM | |
| 700 | 1 | |a Talarico, Emanuela |e verfasserin |4 aut | |
| 700 | 1 | |a Madeo, Maria Letizia |e verfasserin |4 aut | |
| 700 | 1 | |a Greco, Eleonora |e verfasserin |4 aut | |
| 700 | 1 | |a Minervino, Marco |e verfasserin |4 aut | |
| 700 | 1 | |a Álvarez-Rodríguez, Sara |e verfasserin |4 aut | |
| 700 | 1 | |a Muto, Antonella |e verfasserin |4 aut | |
| 700 | 1 | |a Ferrari, Michele |e verfasserin |4 aut | |
| 700 | 1 | |a Chiappetta, Adriana |e verfasserin |4 aut | |
| 700 | 1 | |a Bruno, Leonardo |e verfasserin |4 aut | |
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