Clomipramine inhibits dynamin GTPase activity by L-α-phosphatidyl-L-serine stimulation
© The Author(s) 2023. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved..
Three dynamin isoforms play critical roles in clathrin-dependent endocytosis. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) enters host cells via clathrin-dependent endocytosis. We previously reported that 3-(3-chloro-10,11-dihydro-5H-dibenzo[b,f]azepin-5-yl)-N,N-dimethylpropan-1-amine (clomipramine) inhibits the GTPase activity of dynamin 1, which is in mainly neuron. Therefore, we investigated whether clomipramine inhibits the activity of other dynamin isoforms in this study. We found that, similar to its inhibitory effect on dynamin 1, clomipramine inhibited the l-α-phosphatidyl-l-serine-stimulated GTPase activity of dynamin 2, which is expressed ubiquitously, and dynamin 3, which is expressed in the lung. Inhibition of GTPase activity raises the possibility that clomipramine can suppress SARS-CoV-2 entry into host cells.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:174 |
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| Enthalten in: |
Journal of biochemistry - 174(2023), 3 vom: 31. Juli, Seite 267-272 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Miyoshi, Hiroshi [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 31.08.2023 Date Revised 31.08.2023 published: Print Citation Status MEDLINE |
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| doi: |
10.1093/jb/mvad038 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM356405877 |
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| 245 | 1 | 0 | |a Clomipramine inhibits dynamin GTPase activity by L-α-phosphatidyl-L-serine stimulation |
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| 500 | |a Date Revised 31.08.2023 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © The Author(s) 2023. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved. | ||
| 520 | |a Three dynamin isoforms play critical roles in clathrin-dependent endocytosis. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) enters host cells via clathrin-dependent endocytosis. We previously reported that 3-(3-chloro-10,11-dihydro-5H-dibenzo[b,f]azepin-5-yl)-N,N-dimethylpropan-1-amine (clomipramine) inhibits the GTPase activity of dynamin 1, which is in mainly neuron. Therefore, we investigated whether clomipramine inhibits the activity of other dynamin isoforms in this study. We found that, similar to its inhibitory effect on dynamin 1, clomipramine inhibited the l-α-phosphatidyl-l-serine-stimulated GTPase activity of dynamin 2, which is expressed ubiquitously, and dynamin 3, which is expressed in the lung. Inhibition of GTPase activity raises the possibility that clomipramine can suppress SARS-CoV-2 entry into host cells | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a SARS-CoV-2. Abbreviations: ACE2, angiotensin-converting enzyme 2; Dyn, dynamin; His, histidine; MiTMAB, myristyl trimethyl ammonium bromide; Pi, orthophosphate; PS, l-α-phosphatidyl-l-serine; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2 | |
| 650 | 4 | |a clomipramine | |
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| 650 | 4 | |a inhibition | |
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| 650 | 7 | |a Clomipramine |2 NLM | |
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| 700 | 1 | |a Otomo, Masahiro |e verfasserin |4 aut | |
| 700 | 1 | |a Takahashi, Kiyofumi |e verfasserin |4 aut | |
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