Concise review on the combined use of immunocapture, mass spectrometry and liquid chromatography for clinical applications
© 2023 Walter de Gruyter GmbH, Berlin/Boston..
Immunocapture is now a well-established method for sample preparation prior to quantitation of peptides and proteins in complex matrices. This short review will give an overview of some clinical applications of immunocapture methods, as well as protocols with and without enzymatic digestion in a clinical context. The advantages and limitations of both approaches are discussed in detail. Challenges related to the choice of mass spectrometer are also discussed. Top-down, middle-down, and bottom-up approaches are discussed. Even though immunocapture has its limitations, its main advantage is that it provides an additional dimension of separation and/or isolation when working with peptides and proteins. Overall, this short review demonstrates the potential of such techniques in the field of proteomics-based clinical medicine and paves the way for better personalized medicine.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:61 |
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| Enthalten in: |
Clinical chemistry and laboratory medicine - 61(2023), 10 vom: 26. Sept., Seite 1700-1707 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Massonnet, Philippe [VerfasserIn] |
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| Links: |
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| Themen: |
Immunocapture |
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| Anmerkungen: |
Date Completed 21.08.2023 Date Revised 24.01.2024 published: Electronic-Print Citation Status MEDLINE |
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| doi: |
10.1515/cclm-2023-0253 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
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| 520 | |a Immunocapture is now a well-established method for sample preparation prior to quantitation of peptides and proteins in complex matrices. This short review will give an overview of some clinical applications of immunocapture methods, as well as protocols with and without enzymatic digestion in a clinical context. The advantages and limitations of both approaches are discussed in detail. Challenges related to the choice of mass spectrometer are also discussed. Top-down, middle-down, and bottom-up approaches are discussed. Even though immunocapture has its limitations, its main advantage is that it provides an additional dimension of separation and/or isolation when working with peptides and proteins. Overall, this short review demonstrates the potential of such techniques in the field of proteomics-based clinical medicine and paves the way for better personalized medicine | ||
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| 700 | 1 | |a Farré-Segura, Jordi |e verfasserin |4 aut | |
| 700 | 1 | |a Demeuse, Justine |e verfasserin |4 aut | |
| 700 | 1 | |a Huyghebaert, Loreen |e verfasserin |4 aut | |
| 700 | 1 | |a Dubrowski, Thomas |e verfasserin |4 aut | |
| 700 | 1 | |a Dufour, Patrice |e verfasserin |4 aut | |
| 700 | 1 | |a Schoumacher, Matthieu |e verfasserin |4 aut | |
| 700 | 1 | |a Peeters, Stéphanie |e verfasserin |4 aut | |
| 700 | 1 | |a Le Goff, Caroline |e verfasserin |4 aut | |
| 700 | 1 | |a Cavalier, Etienne |e verfasserin |4 aut | |
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