Identifying the core genome of the nucleus-forming bacteriophage family and characterization of Erwinia phage RAY
Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved..
We recently discovered that some bacteriophages establish a nucleus-like replication compartment (phage nucleus), but the core genes that define nucleus-based phage replication and their phylogenetic distribution were still to be determined. Here, we show that phages encoding the major phage nucleus protein chimallin share 72 conserved genes encoded within seven gene blocks. Of these, 21 core genes are unique to nucleus-forming phage, and all but one of these genes encode proteins of unknown function. We propose that these phages comprise a novel viral family we term Chimalliviridae. Fluorescence microscopy and cryoelectron tomography studies of Erwinia phage vB_EamM_RAY confirm that many of the key steps of nucleus-based replication are conserved among diverse chimalliviruses and reveal variations on this replication mechanism. This work expands our understanding of phage nucleus and PhuZ spindle diversity and function, providing a roadmap for identifying key mechanisms underlying nucleus-based phage replication.
| Errataetall: | |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:42 |
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| Enthalten in: |
Cell reports - 42(2023), 5 vom: 30. Mai, Seite 112432 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Prichard, Amy [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 05.06.2023 Date Revised 09.11.2023 published: Print-Electronic UpdateOf: bioRxiv. 2023 Feb 25;:. - PMID 36865095 Citation Status MEDLINE |
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| doi: |
10.1016/j.celrep.2023.112432 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM356242064 |
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| 100 | 1 | |a Prichard, Amy |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Identifying the core genome of the nucleus-forming bacteriophage family and characterization of Erwinia phage RAY |
| 264 | 1 | |c 2023 | |
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| 500 | |a Date Completed 05.06.2023 | ||
| 500 | |a Date Revised 09.11.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a UpdateOf: bioRxiv. 2023 Feb 25;:. - PMID 36865095 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a We recently discovered that some bacteriophages establish a nucleus-like replication compartment (phage nucleus), but the core genes that define nucleus-based phage replication and their phylogenetic distribution were still to be determined. Here, we show that phages encoding the major phage nucleus protein chimallin share 72 conserved genes encoded within seven gene blocks. Of these, 21 core genes are unique to nucleus-forming phage, and all but one of these genes encode proteins of unknown function. We propose that these phages comprise a novel viral family we term Chimalliviridae. Fluorescence microscopy and cryoelectron tomography studies of Erwinia phage vB_EamM_RAY confirm that many of the key steps of nucleus-based replication are conserved among diverse chimalliviruses and reveal variations on this replication mechanism. This work expands our understanding of phage nucleus and PhuZ spindle diversity and function, providing a roadmap for identifying key mechanisms underlying nucleus-based phage replication | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a CP: Microbiology | |
| 650 | 4 | |a Chimallin | |
| 650 | 4 | |a Erwinia | |
| 650 | 4 | |a PhuZ | |
| 650 | 4 | |a core genome | |
| 650 | 4 | |a cytoskeleton | |
| 650 | 4 | |a nuclear shell | |
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| 650 | 4 | |a phage nucleus | |
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| 700 | 1 | |a Lee, Jina |e verfasserin |4 aut | |
| 700 | 1 | |a Laughlin, Thomas G |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Amber |e verfasserin |4 aut | |
| 700 | 1 | |a Thomas, Kyle P |e verfasserin |4 aut | |
| 700 | 1 | |a Sy, Annika E |e verfasserin |4 aut | |
| 700 | 1 | |a Spencer, Tara |e verfasserin |4 aut | |
| 700 | 1 | |a Asavavimol, Aileen |e verfasserin |4 aut | |
| 700 | 1 | |a Cafferata, Allison |e verfasserin |4 aut | |
| 700 | 1 | |a Cameron, Mia |e verfasserin |4 aut | |
| 700 | 1 | |a Chiu, Nicholas |e verfasserin |4 aut | |
| 700 | 1 | |a Davydov, Demyan |e verfasserin |4 aut | |
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| 700 | 1 | |a Diaz, Gabriel |e verfasserin |4 aut | |
| 700 | 1 | |a Guereca, Melissa |e verfasserin |4 aut | |
| 700 | 1 | |a Hearst, Kiley |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Leyi |e verfasserin |4 aut | |
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| 700 | 1 | |a Johnson, Annika |e verfasserin |4 aut | |
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| 700 | 1 | |a Martinez, Adamari |e verfasserin |4 aut | |
| 700 | 1 | |a Norquist, Meliné |e verfasserin |4 aut | |
| 700 | 1 | |a Pau, Tyler |e verfasserin |4 aut | |
| 700 | 1 | |a Prasad, Gino |e verfasserin |4 aut | |
| 700 | 1 | |a Saam, Katrina |e verfasserin |4 aut | |
| 700 | 1 | |a Sandhu, Milan |e verfasserin |4 aut | |
| 700 | 1 | |a Sarabia, Angel Jose |e verfasserin |4 aut | |
| 700 | 1 | |a Schumaker, Siena |e verfasserin |4 aut | |
| 700 | 1 | |a Sonin, Aaron |e verfasserin |4 aut | |
| 700 | 1 | |a Uyeno, Ariya |e verfasserin |4 aut | |
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| 700 | 1 | |a Pogliano, Joe |e verfasserin |4 aut | |
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