Glycyrrhizae Radix suppresses lipopolysaccharide- and diazepam-induced nerve inflammation in the hippocampus, and contracts the duration of pentobarbital- induced loss of righting reflex in a mouse model
© 2023. The Author(s) under exclusive licence to The Japanese Society of Pharmacognosy..
Nerve inflammation is linked to the development of various neurological disorders. This study aimed to examine whether Glycyrrhizae Radix effectively influences the duration of the pentobarbital-induced loss of righting reflex, which may increase in a mouse model of lipopolysaccharide (LPS)-induced nerve inflammation and diazepam-induced γ-aminobutyric acid receptor hypersensitivity. Furthermore, we examined the anti-inflammatory effects of Glycyrrhizae Radix extract on LPS-stimulated BV2 microglial cells, in vitro. Treatment with Glycyrrhizae Radix significantly decreased the duration of pentobarbital-induced loss of righting reflex in the mouse model. Furthermore, treatment with Glycyrrhizae Radix significantly attenuated the LPS-induced increases in interleukin-1β, interleukin-6, and tumor necrosis factor-alpha at the mRNA level, and it significantly reduced the number of ionized calcium-binding adapter molecule-1-positive cells in the hippocampal dentate gyrus 24 h after LPS treatment. Treatment with Glycyrrhizae Radix also suppressed the release of nitric oxide, interleukin-1β, interleukin-6, and tumor necrosis factor protein in culture supernatants of LPS-stimulated BV2 cells. In addition, glycyrrhizic acid and liquiritin, active ingredients of Glycyrrhizae Radix extract, reduced the duration of pentobarbital-induced loss of righting reflex. These findings suggest that Glycyrrhizae Radix, as well as its active ingredients, glycyrrhizic acid and liquiritin, may be effective therapeutic agents for the treatment of nerve inflammation-induced neurological disorders.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:77 |
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| Enthalten in: |
Journal of natural medicines - 77(2023), 3 vom: 11. Juni, Seite 561-571 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kawada, Kei [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 31.05.2023 Date Revised 31.05.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1007/s11418-023-01700-2 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM356190382 |
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| 100 | 1 | |a Kawada, Kei |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Glycyrrhizae Radix suppresses lipopolysaccharide- and diazepam-induced nerve inflammation in the hippocampus, and contracts the duration of pentobarbital- induced loss of righting reflex in a mouse model |
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| 500 | |a Date Revised 31.05.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2023. The Author(s) under exclusive licence to The Japanese Society of Pharmacognosy. | ||
| 520 | |a Nerve inflammation is linked to the development of various neurological disorders. This study aimed to examine whether Glycyrrhizae Radix effectively influences the duration of the pentobarbital-induced loss of righting reflex, which may increase in a mouse model of lipopolysaccharide (LPS)-induced nerve inflammation and diazepam-induced γ-aminobutyric acid receptor hypersensitivity. Furthermore, we examined the anti-inflammatory effects of Glycyrrhizae Radix extract on LPS-stimulated BV2 microglial cells, in vitro. Treatment with Glycyrrhizae Radix significantly decreased the duration of pentobarbital-induced loss of righting reflex in the mouse model. Furthermore, treatment with Glycyrrhizae Radix significantly attenuated the LPS-induced increases in interleukin-1β, interleukin-6, and tumor necrosis factor-alpha at the mRNA level, and it significantly reduced the number of ionized calcium-binding adapter molecule-1-positive cells in the hippocampal dentate gyrus 24 h after LPS treatment. Treatment with Glycyrrhizae Radix also suppressed the release of nitric oxide, interleukin-1β, interleukin-6, and tumor necrosis factor protein in culture supernatants of LPS-stimulated BV2 cells. In addition, glycyrrhizic acid and liquiritin, active ingredients of Glycyrrhizae Radix extract, reduced the duration of pentobarbital-induced loss of righting reflex. These findings suggest that Glycyrrhizae Radix, as well as its active ingredients, glycyrrhizic acid and liquiritin, may be effective therapeutic agents for the treatment of nerve inflammation-induced neurological disorders | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Glycyrrhizae Radix | |
| 650 | 4 | |a Glycyrrhizic acid | |
| 650 | 4 | |a Liquiritin | |
| 650 | 4 | |a Loss of righting reflex | |
| 650 | 4 | |a Mouse model | |
| 650 | 4 | |a Nerve inflammation | |
| 650 | 7 | |a Interleukin-6 |2 NLM | |
| 650 | 7 | |a Lipopolysaccharides |2 NLM | |
| 650 | 7 | |a Glycyrrhizic Acid |2 NLM | |
| 650 | 7 | |a 6FO62043WK |2 NLM | |
| 650 | 7 | |a Pentobarbital |2 NLM | |
| 650 | 7 | |a I4744080IR |2 NLM | |
| 650 | 7 | |a Interleukin-1beta |2 NLM | |
| 650 | 7 | |a Diazepam |2 NLM | |
| 650 | 7 | |a Q3JTX2Q7TU |2 NLM | |
| 650 | 7 | |a Plant Extracts |2 NLM | |
| 650 | 7 | |a Drugs, Chinese Herbal |2 NLM | |
| 700 | 1 | |a Ishida, Tomoaki |e verfasserin |4 aut | |
| 700 | 1 | |a Jobu, Kohei |e verfasserin |4 aut | |
| 700 | 1 | |a Morisawa, Shumpei |e verfasserin |4 aut | |
| 700 | 1 | |a Nishida, Motoki |e verfasserin |4 aut | |
| 700 | 1 | |a Tamura, Naohisa |e verfasserin |4 aut | |
| 700 | 1 | |a Yoshioka, Saburo |e verfasserin |4 aut | |
| 700 | 1 | |a Miyamura, Mitsuhiko |e verfasserin |4 aut | |
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