The Acute Effects and Mechanism of Ketamine on Nicotine-Induced Neurogenic Relaxation of the Corpus Cavernosum in Mice
The present study aimed to investigate the acute effects and the mechanism of ketamine on nicotine-induced relaxation of the corpus cavernosum (CC) in mice. This study measured the intra-cavernosal pressure (ICP) of male C57BL/6 mice and the CC muscle activities using an organ bath wire myograph. Various drugs were used to investigate the mechanism of ketamine on nicotine-induced relaxation. Direct ketamine injection into the major pelvic ganglion (MPG) inhibited MPG-induced increases in ICP. D-serine/L-glutamate-induced relaxation of the CC was inhibited by MK-801 (N-methyl-D-aspartate (NMDA) receptor inhibitor), and nicotine-induced relaxation was enhanced by D-serine/L-glutamate. NMDA had no effect on CC relaxation. Nicotine-induced relaxation of the CC was suppressed by mecamylamine (a non-selective nicotinic acetylcholine receptor antagonist), lidocaine, guanethidine (an adrenergic neuronal blocker), Nw-nitro-L-arginine (a non-selective nitric oxide synthase inhibitor), MK-801, and ketamine. This relaxation was almost completely inhibited in CC strips pretreated with 6-hydroxydopamine (a neurotoxic synthetic organic compound). Ketamine inhibited cavernosal nerve neurotransmission via direct action on the ganglion and impaired nicotine-induced CC relaxation. The relaxation of the CC was dependent on the interaction of the sympathetic and parasympathetic nerves, which may be mediated by the NMDA receptor.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2023 |
---|---|
Erschienen: |
2023 |
Enthalten in: |
Zur Gesamtaufnahme - volume:24 |
---|---|
Enthalten in: |
International journal of molecular sciences - 24(2023), 8 vom: 10. Apr. |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Li, Ming-Wei [VerfasserIn] |
---|
Links: |
---|
Anmerkungen: |
Date Completed 01.05.2023 Date Revised 01.05.2023 published: Electronic Citation Status MEDLINE |
---|
doi: |
10.3390/ijms24086976 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM356117146 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM356117146 | ||
003 | DE-627 | ||
005 | 20231226065800.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231226s2023 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.3390/ijms24086976 |2 doi | |
028 | 5 | 2 | |a pubmed24n1187.xml |
035 | |a (DE-627)NLM356117146 | ||
035 | |a (NLM)37108139 | ||
035 | |a (PII)6976 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Li, Ming-Wei |e verfasserin |4 aut | |
245 | 1 | 4 | |a The Acute Effects and Mechanism of Ketamine on Nicotine-Induced Neurogenic Relaxation of the Corpus Cavernosum in Mice |
264 | 1 | |c 2023 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 01.05.2023 | ||
500 | |a Date Revised 01.05.2023 | ||
500 | |a published: Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a The present study aimed to investigate the acute effects and the mechanism of ketamine on nicotine-induced relaxation of the corpus cavernosum (CC) in mice. This study measured the intra-cavernosal pressure (ICP) of male C57BL/6 mice and the CC muscle activities using an organ bath wire myograph. Various drugs were used to investigate the mechanism of ketamine on nicotine-induced relaxation. Direct ketamine injection into the major pelvic ganglion (MPG) inhibited MPG-induced increases in ICP. D-serine/L-glutamate-induced relaxation of the CC was inhibited by MK-801 (N-methyl-D-aspartate (NMDA) receptor inhibitor), and nicotine-induced relaxation was enhanced by D-serine/L-glutamate. NMDA had no effect on CC relaxation. Nicotine-induced relaxation of the CC was suppressed by mecamylamine (a non-selective nicotinic acetylcholine receptor antagonist), lidocaine, guanethidine (an adrenergic neuronal blocker), Nw-nitro-L-arginine (a non-selective nitric oxide synthase inhibitor), MK-801, and ketamine. This relaxation was almost completely inhibited in CC strips pretreated with 6-hydroxydopamine (a neurotoxic synthetic organic compound). Ketamine inhibited cavernosal nerve neurotransmission via direct action on the ganglion and impaired nicotine-induced CC relaxation. The relaxation of the CC was dependent on the interaction of the sympathetic and parasympathetic nerves, which may be mediated by the NMDA receptor | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a N-methyl-D-aspartate | |
650 | 4 | |a corpus cavernosum | |
650 | 4 | |a ketamine | |
650 | 4 | |a major pelvic ganglion | |
650 | 4 | |a sympathetic nerve | |
650 | 7 | |a Nicotine |2 NLM | |
650 | 7 | |a 6M3C89ZY6R |2 NLM | |
650 | 7 | |a Ketamine |2 NLM | |
650 | 7 | |a 690G0D6V8H |2 NLM | |
650 | 7 | |a Glutamic Acid |2 NLM | |
650 | 7 | |a 3KX376GY7L |2 NLM | |
650 | 7 | |a N-Methylaspartate |2 NLM | |
650 | 7 | |a 6384-92-5 |2 NLM | |
650 | 7 | |a Dizocilpine Maleate |2 NLM | |
650 | 7 | |a 6LR8C1B66Q |2 NLM | |
650 | 7 | |a Serine |2 NLM | |
650 | 7 | |a 452VLY9402 |2 NLM | |
650 | 7 | |a Nitric Oxide |2 NLM | |
650 | 7 | |a 31C4KY9ESH |2 NLM | |
700 | 1 | |a Chao, Tze-Chen |e verfasserin |4 aut | |
700 | 1 | |a Lim, Li-Yi |e verfasserin |4 aut | |
700 | 1 | |a Chang, Hsi-Hsien |e verfasserin |4 aut | |
700 | 1 | |a Yang, Stephen Shei-Dei |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t International journal of molecular sciences |d 2008 |g 24(2023), 8 vom: 10. Apr. |w (DE-627)NLM185552161 |x 1422-0067 |7 nnns |
773 | 1 | 8 | |g volume:24 |g year:2023 |g number:8 |g day:10 |g month:04 |
856 | 4 | 0 | |u http://dx.doi.org/10.3390/ijms24086976 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 24 |j 2023 |e 8 |b 10 |c 04 |