Reducing brassinosteroid signalling enhances grain yield in semi-dwarf wheat
© 2023. The Author(s)..
Modern green revolution varieties of wheat (Triticum aestivum L.) confer semi-dwarf and lodging-resistant plant architecture owing to the Reduced height-B1b (Rht-B1b) and Rht-D1b alleles1. However, both Rht-B1b and Rht-D1b are gain-of-function mutant alleles encoding gibberellin signalling repressors that stably repress plant growth and negatively affect nitrogen-use efficiency and grain filling2-5. Therefore, the green revolution varieties of wheat harbouring Rht-B1b or Rht-D1b usually produce smaller grain and require higher nitrogen fertilizer inputs to maintain their grain yields. Here we describe a strategy to design semi-dwarf wheat varieties without the need for Rht-B1b or Rht-D1b alleles. We discovered that absence of Rht-B1 and ZnF-B (encoding a RING-type E3 ligase) through a natural deletion of a haploblock of about 500 kilobases shaped semi-dwarf plants with more compact plant architecture and substantially improved grain yield (up to 15.2%) in field trials. Further genetic analysis confirmed that the deletion of ZnF-B induced the semi-dwarf trait in the absence of the Rht-B1b and Rht-D1b alleles through attenuating brassinosteroid (BR) perception. ZnF acts as a BR signalling activator to facilitate proteasomal destruction of the BR signalling repressor BRI1 kinase inhibitor 1 (TaBKI1), and loss of ZnF stabilizes TaBKI1 to block BR signalling transduction. Our findings not only identified a pivotal BR signalling modulator but also provided a creative strategy to design high-yield semi-dwarf wheat varieties by manipulating the BR signal pathway to sustain wheat production.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:617 |
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| Enthalten in: |
Nature - 617(2023), 7959 vom: 26. Mai, Seite 118-124 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Song, Long [VerfasserIn] |
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| Links: |
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| Themen: |
Brassinosteroids |
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| Anmerkungen: |
Date Completed 17.05.2023 Date Revised 11.12.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s41586-023-06023-6 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 245 | 1 | 0 | |a Reducing brassinosteroid signalling enhances grain yield in semi-dwarf wheat |
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| 520 | |a © 2023. The Author(s). | ||
| 520 | |a Modern green revolution varieties of wheat (Triticum aestivum L.) confer semi-dwarf and lodging-resistant plant architecture owing to the Reduced height-B1b (Rht-B1b) and Rht-D1b alleles1. However, both Rht-B1b and Rht-D1b are gain-of-function mutant alleles encoding gibberellin signalling repressors that stably repress plant growth and negatively affect nitrogen-use efficiency and grain filling2-5. Therefore, the green revolution varieties of wheat harbouring Rht-B1b or Rht-D1b usually produce smaller grain and require higher nitrogen fertilizer inputs to maintain their grain yields. Here we describe a strategy to design semi-dwarf wheat varieties without the need for Rht-B1b or Rht-D1b alleles. We discovered that absence of Rht-B1 and ZnF-B (encoding a RING-type E3 ligase) through a natural deletion of a haploblock of about 500 kilobases shaped semi-dwarf plants with more compact plant architecture and substantially improved grain yield (up to 15.2%) in field trials. Further genetic analysis confirmed that the deletion of ZnF-B induced the semi-dwarf trait in the absence of the Rht-B1b and Rht-D1b alleles through attenuating brassinosteroid (BR) perception. ZnF acts as a BR signalling activator to facilitate proteasomal destruction of the BR signalling repressor BRI1 kinase inhibitor 1 (TaBKI1), and loss of ZnF stabilizes TaBKI1 to block BR signalling transduction. Our findings not only identified a pivotal BR signalling modulator but also provided a creative strategy to design high-yield semi-dwarf wheat varieties by manipulating the BR signal pathway to sustain wheat production | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Brassinosteroids |2 NLM | |
| 650 | 7 | |a Gibberellins |2 NLM | |
| 650 | 7 | |a Plant Proteins |2 NLM | |
| 700 | 1 | |a Liu, Jie |e verfasserin |4 aut | |
| 700 | 1 | |a Cao, Beilu |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Bin |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Xiaoping |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Zhaoyan |e verfasserin |4 aut | |
| 700 | 1 | |a Dong, Chaoqun |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Xiangqing |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Zhaoheng |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Wenxi |e verfasserin |4 aut | |
| 700 | 1 | |a Chai, Lingling |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Jing |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Jun |e verfasserin |4 aut | |
| 700 | 1 | |a Cui, Shubin |e verfasserin |4 aut | |
| 700 | 1 | |a He, Fei |e verfasserin |4 aut | |
| 700 | 1 | |a Peng, Huiru |e verfasserin |4 aut | |
| 700 | 1 | |a Hu, Zhaorong |e verfasserin |4 aut | |
| 700 | 1 | |a Su, Zhenqi |e verfasserin |4 aut | |
| 700 | 1 | |a Guo, Weilong |e verfasserin |4 aut | |
| 700 | 1 | |a Xin, Mingming |e verfasserin |4 aut | |
| 700 | 1 | |a Yao, Yingyin |e verfasserin |4 aut | |
| 700 | 1 | |a Yan, Yong |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Yinming |e verfasserin |4 aut | |
| 700 | 1 | |a Bai, Guihua |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Qixin |e verfasserin |4 aut | |
| 700 | 1 | |a Ni, Zhongfu |e verfasserin |4 aut | |
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