Single cell landscape of parietal epithelial cells in healthy and diseased states
Copyright © 2023 International Society of Nephrology. Published by Elsevier Inc. All rights reserved..
The biology and diversity of glomerular parietal epithelial cells (PECs) are important for understanding podocyte regeneration and crescent formation. Although protein markers have revealed the morphological heterogeneity of PECs, the molecular characteristics of PEC subpopulations remain largely unknown. Here, we performed a comprehensive analysis of PECs using single-cell RNA sequencing (scRNA-seq) data. Our analysis identified five distinct PEC subpopulations: PEC-A1, PEC-A2, PEC-A3, PEC-A4 and PEC-B. Among these subpopulations, PEC- A1 and PEC-A2 were characterized as podocyte progenitors while PEC-A4 represented tubular progenitors. Further dynamic signaling network analysis indicated that activation of PEC-A4 and the proliferation of PEC-A3 played pivotal roles in crescent formation. Analyses suggested that upstream signals released by podocytes, immune cells, endothelial cells and mesangial cells serve as pathogenic signals and may be promising intervention targets in crescentic glomerulonephritis. Pharmacological blockade of two such pathogenic signaling targets, proteins Mif and Csf1r, reduced hyperplasia of the PECs and crescent formation in anti-glomerular basement membrane glomerulonephritis murine models. Thus, our study demonstrates that scRNA-seq-based analysis provided valuable insights into the pathology and therapeutic strategies for crescentic glomerulonephritis.
| Errataetall: |
CommentIn: Kidney Int. 2023 Jul;104(1):33-35. - PMID 37349059 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:104 |
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| Enthalten in: |
Kidney international - 104(2023), 1 vom: 24. Juli, Seite 108-123 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Liu, Wen-Bin [VerfasserIn] |
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| Links: |
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| Themen: |
Anti-GBM disease |
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| Anmerkungen: |
Date Completed 26.06.2023 Date Revised 22.11.2023 published: Print-Electronic CommentIn: Kidney Int. 2023 Jul;104(1):33-35. - PMID 37349059 Citation Status MEDLINE |
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| doi: |
10.1016/j.kint.2023.03.036 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Copyright © 2023 International Society of Nephrology. Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a The biology and diversity of glomerular parietal epithelial cells (PECs) are important for understanding podocyte regeneration and crescent formation. Although protein markers have revealed the morphological heterogeneity of PECs, the molecular characteristics of PEC subpopulations remain largely unknown. Here, we performed a comprehensive analysis of PECs using single-cell RNA sequencing (scRNA-seq) data. Our analysis identified five distinct PEC subpopulations: PEC-A1, PEC-A2, PEC-A3, PEC-A4 and PEC-B. Among these subpopulations, PEC- A1 and PEC-A2 were characterized as podocyte progenitors while PEC-A4 represented tubular progenitors. Further dynamic signaling network analysis indicated that activation of PEC-A4 and the proliferation of PEC-A3 played pivotal roles in crescent formation. Analyses suggested that upstream signals released by podocytes, immune cells, endothelial cells and mesangial cells serve as pathogenic signals and may be promising intervention targets in crescentic glomerulonephritis. Pharmacological blockade of two such pathogenic signaling targets, proteins Mif and Csf1r, reduced hyperplasia of the PECs and crescent formation in anti-glomerular basement membrane glomerulonephritis murine models. Thus, our study demonstrates that scRNA-seq-based analysis provided valuable insights into the pathology and therapeutic strategies for crescentic glomerulonephritis | ||
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| 700 | 1 | |a Geng, Jie |e verfasserin |4 aut | |
| 700 | 1 | |a Rui, Hong-Liang |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, Chang |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Yu-Jiao |e verfasserin |4 aut | |
| 700 | 1 | |a Huo, Gui-Yang |e verfasserin |4 aut | |
| 700 | 1 | |a Mao, Jia-Rong |e verfasserin |4 aut | |
| 700 | 1 | |a Lu, Chuan-Jian |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, An-Long |e verfasserin |4 aut | |
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