Accurate determination of the milk protein allergen β-lactoglobulin by on-line aptamer affinity solid-phase extraction capillary electrophoresis-mass spectrometry
Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved..
An on-line aptamer affinity solid-phase extraction capillary electrophoresis-mass spectrometry (AA-SPE-CE-MS) method was developed to purify, preconcentrate, separate, and characterize the milk allergenic protein β-lactoglobulin (β-LG) in food samples. The sorbent to pack into the SPE microcartidges was prepared by immobilizing an aptamer against β-LG onto magnetic bead particles. After optimizing the SPE-CE-MS method, the sample (ca. 75 μL) was loaded in separation background electrolyte (BGE, 2 M acetic acid pH 2.2), while a solution of 100 mM NH4OH (pH 11.2) (ca. 100 nL) was used for the protein elution. The linearity of the method ranged between 0.1 and 20 μg mL-1 and the limit of detection (LOD) was 0.05 μg mL-1, which was 200 times lower than by CE-MS. The method was repeatable in terms of relative standard deviation (RSD) for migration times and peak areas (<0.5% and 2.4%, respectively) and microcartridge lifetime was more than 25 analyses. The applicability of the method for the determination of low levels of β-LG was shown by analyzing milk-free foods (i.e. a 100% cocoa dark chocolate, a hypoallergenic formula for infants, and a dairy-free white bread) and milk-containing white breads. Results were satisfactory in all cases, thus demonstrating the great potential of the developed method for accurate food safety and quality control.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:259 |
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| Enthalten in: |
Talanta - 259(2023) vom: 01. Juli, Seite 124542 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Vergara-Barberán, María [VerfasserIn] |
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| Links: |
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| Themen: |
β-lactoglobulin |
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| Anmerkungen: |
Date Completed 01.05.2023 Date Revised 01.05.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.talanta.2023.124542 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM35590411X |
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| 245 | 1 | 0 | |a Accurate determination of the milk protein allergen β-lactoglobulin by on-line aptamer affinity solid-phase extraction capillary electrophoresis-mass spectrometry |
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| 500 | |a published: Print-Electronic | ||
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| 520 | |a Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved. | ||
| 520 | |a An on-line aptamer affinity solid-phase extraction capillary electrophoresis-mass spectrometry (AA-SPE-CE-MS) method was developed to purify, preconcentrate, separate, and characterize the milk allergenic protein β-lactoglobulin (β-LG) in food samples. The sorbent to pack into the SPE microcartidges was prepared by immobilizing an aptamer against β-LG onto magnetic bead particles. After optimizing the SPE-CE-MS method, the sample (ca. 75 μL) was loaded in separation background electrolyte (BGE, 2 M acetic acid pH 2.2), while a solution of 100 mM NH4OH (pH 11.2) (ca. 100 nL) was used for the protein elution. The linearity of the method ranged between 0.1 and 20 μg mL-1 and the limit of detection (LOD) was 0.05 μg mL-1, which was 200 times lower than by CE-MS. The method was repeatable in terms of relative standard deviation (RSD) for migration times and peak areas (<0.5% and 2.4%, respectively) and microcartridge lifetime was more than 25 analyses. The applicability of the method for the determination of low levels of β-LG was shown by analyzing milk-free foods (i.e. a 100% cocoa dark chocolate, a hypoallergenic formula for infants, and a dairy-free white bread) and milk-containing white breads. Results were satisfactory in all cases, thus demonstrating the great potential of the developed method for accurate food safety and quality control | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Aptamer | |
| 650 | 4 | |a Capillary electrophoresis | |
| 650 | 4 | |a In-line solid-phase extraction | |
| 650 | 4 | |a Mass spectrometry | |
| 650 | 4 | |a Milk protein allergen | |
| 650 | 4 | |a β-lactoglobulin | |
| 650 | 7 | |a Milk Proteins |2 NLM | |
| 650 | 7 | |a Lactoglobulins |2 NLM | |
| 650 | 7 | |a Allergens |2 NLM | |
| 650 | 7 | |a Oligonucleotides |2 NLM | |
| 700 | 1 | |a Simó-Alfonso, Ernesto Francisco |e verfasserin |4 aut | |
| 700 | 1 | |a Herrero-Martínez, José Manuel |e verfasserin |4 aut | |
| 700 | 1 | |a Benavente, Fernando |e verfasserin |4 aut | |
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