The Smc5/6 complex is a DNA loop-extruding motor
© 2023. The Author(s)..
Structural maintenance of chromosomes (SMC) protein complexes are essential for the spatial organization of chromosomes1. Whereas cohesin and condensin organize chromosomes by extrusion of DNA loops, the molecular functions of the third eukaryotic SMC complex, Smc5/6, remain largely unknown2. Using single-molecule imaging, we show that Smc5/6 forms DNA loops by extrusion. Upon ATP hydrolysis, Smc5/6 reels DNA symmetrically into loops at a force-dependent rate of one kilobase pair per second. Smc5/6 extrudes loops in the form of dimers, whereas monomeric Smc5/6 unidirectionally translocates along DNA. We also find that the subunits Nse5 and Nse6 (Nse5/6) act as negative regulators of loop extrusion. Nse5/6 inhibits loop-extrusion initiation by hindering Smc5/6 dimerization but has no influence on ongoing loop extrusion. Our findings reveal functions of Smc5/6 at the molecular level and establish DNA loop extrusion as a conserved mechanism among eukaryotic SMC complexes.
| Errataetall: |
CommentIn: Nat Struct Mol Biol. 2023 May;30(5):567. - PMID 37198271 |
|---|---|
| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:616 |
|---|---|
| Enthalten in: |
Nature - 616(2023), 7958 vom: 22. Apr., Seite 843-848 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Pradhan, Biswajit [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 22.08.2023 Date Revised 13.12.2023 published: Print-Electronic CommentIn: Nat Struct Mol Biol. 2023 May;30(5):567. - PMID 37198271 Citation Status MEDLINE |
|---|
| doi: |
10.1038/s41586-023-05963-3 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM35580445X |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM35580445X | ||
| 003 | DE-627 | ||
| 005 | 20231227131220.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/s41586-023-05963-3 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1225.xml |
| 035 | |a (DE-627)NLM35580445X | ||
| 035 | |a (NLM)37076626 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Pradhan, Biswajit |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The Smc5/6 complex is a DNA loop-extruding motor |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 22.08.2023 | ||
| 500 | |a Date Revised 13.12.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a CommentIn: Nat Struct Mol Biol. 2023 May;30(5):567. - PMID 37198271 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2023. The Author(s). | ||
| 520 | |a Structural maintenance of chromosomes (SMC) protein complexes are essential for the spatial organization of chromosomes1. Whereas cohesin and condensin organize chromosomes by extrusion of DNA loops, the molecular functions of the third eukaryotic SMC complex, Smc5/6, remain largely unknown2. Using single-molecule imaging, we show that Smc5/6 forms DNA loops by extrusion. Upon ATP hydrolysis, Smc5/6 reels DNA symmetrically into loops at a force-dependent rate of one kilobase pair per second. Smc5/6 extrudes loops in the form of dimers, whereas monomeric Smc5/6 unidirectionally translocates along DNA. We also find that the subunits Nse5 and Nse6 (Nse5/6) act as negative regulators of loop extrusion. Nse5/6 inhibits loop-extrusion initiation by hindering Smc5/6 dimerization but has no influence on ongoing loop extrusion. Our findings reveal functions of Smc5/6 at the molecular level and establish DNA loop extrusion as a conserved mechanism among eukaryotic SMC complexes | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Adenosine Triphosphate |2 NLM | |
| 650 | 7 | |a 8L70Q75FXE |2 NLM | |
| 650 | 7 | |a Cell Cycle Proteins |2 NLM | |
| 650 | 7 | |a Chromosomal Proteins, Non-Histone |2 NLM | |
| 650 | 7 | |a condensin complexes |2 NLM | |
| 650 | 7 | |a DNA, Fungal |2 NLM | |
| 650 | 7 | |a Multiprotein Complexes |2 NLM | |
| 650 | 7 | |a NSE5 protein, S cerevisiae |2 NLM | |
| 650 | 7 | |a SMC5 protein, S cerevisiae |2 NLM | |
| 650 | 7 | |a SMC6 protein, S cerevisiae |2 NLM | |
| 650 | 7 | |a Nse6 protein, S cerevisiae |2 NLM | |
| 700 | 1 | |a Kanno, Takaharu |e verfasserin |4 aut | |
| 700 | 1 | |a Umeda Igarashi, Miki |e verfasserin |4 aut | |
| 700 | 1 | |a Loke, Mun Siong |e verfasserin |4 aut | |
| 700 | 1 | |a Baaske, Martin Dieter |e verfasserin |4 aut | |
| 700 | 1 | |a Wong, Jan Siu Kei |e verfasserin |4 aut | |
| 700 | 1 | |a Jeppsson, Kristian |e verfasserin |4 aut | |
| 700 | 1 | |a Björkegren, Camilla |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Eugene |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Nature |d 1945 |g 616(2023), 7958 vom: 22. Apr., Seite 843-848 |w (DE-627)NLM000008257 |x 1476-4687 |7 nnns |
| 773 | 1 | 8 | |g volume:616 |g year:2023 |g number:7958 |g day:22 |g month:04 |g pages:843-848 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1038/s41586-023-05963-3 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 616 |j 2023 |e 7958 |b 22 |c 04 |h 843-848 | ||