A multi-site, international laboratory study to assess the performance of penicillin susceptibility testing of Staphylococcus aureus
© The Author(s) 2023. Published by Oxford University Press on behalf of British Society for Antimicrobial Chemotherapy. All rights reserved. For permissions, please e-mail: journals.permissionsoup.com..
OBJECTIVES: There is clinical uncertainty over the optimal treatment for penicillin-susceptible Staphylococcus aureus (PSSA) infections. Furthermore, there is concern that phenotypic penicillin susceptibility testing methods are not reliably able to detect some blaZ-positive S. aureus.
METHODS: Nine S. aureus isolates, including six genetically diverse strains harbouring blaZ, were sent in triplicate to 34 participating laboratories from Australia (n = 14), New Zealand (n = 6), Canada (n = 12), Singapore (n = 1) and Israel (n = 1). We used blaZ PCR as the gold standard to assess susceptibility testing performance of CLSI (P10 disc) and EUCAST (P1 disc) methods. Very major errors (VMEs), major error (MEs) and categorical agreement were calculated.
RESULTS: Twenty-two laboratories reported 593 results according to CLSI methodology (P10 disc). Nineteen laboratories reported 513 results according to the EUCAST (P1 disc) method. For CLSI laboratories, the categorical agreement and calculated VME and ME rates were 85% (508/593), 21% (84/396) and 1.5% (3/198), respectively. For EUCAST laboratories, the categorical agreement and calculated VME and ME rates were 93% (475/513), 11% (84/396) and 1% (3/198), respectively. Seven laboratories reported results for both methods, with VME rates of 24% for CLSI and 12% for EUCAST.
CONCLUSIONS: The EUCAST method with a P1 disc resulted in a lower VME rate compared with the CLSI methods with a P10 disc. These results should be considered in the context that among collections of PSSA isolates, as determined by automated MIC testing, less than 10% harbour blaZ. Furthermore, the clinical relevance of phenotypically susceptible, but blaZ-positive S. aureus, remains unclear.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:78 |
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| Enthalten in: |
The Journal of antimicrobial chemotherapy - 78(2023), 6 vom: 01. Juni, Seite 1499-1504 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Henderson, Andrew [VerfasserIn] |
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| Links: |
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| Themen: |
Anti-Bacterial Agents |
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| Anmerkungen: |
Date Completed 02.06.2023 Date Revised 19.04.2024 published: Print Citation Status MEDLINE |
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| doi: |
10.1093/jac/dkad116 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM355754894 |
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| 100 | 1 | |a Henderson, Andrew |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A multi-site, international laboratory study to assess the performance of penicillin susceptibility testing of Staphylococcus aureus |
| 264 | 1 | |c 2023 | |
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| 500 | |a Date Completed 02.06.2023 | ||
| 500 | |a Date Revised 19.04.2024 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © The Author(s) 2023. Published by Oxford University Press on behalf of British Society for Antimicrobial Chemotherapy. All rights reserved. For permissions, please e-mail: journals.permissionsoup.com. | ||
| 520 | |a OBJECTIVES: There is clinical uncertainty over the optimal treatment for penicillin-susceptible Staphylococcus aureus (PSSA) infections. Furthermore, there is concern that phenotypic penicillin susceptibility testing methods are not reliably able to detect some blaZ-positive S. aureus | ||
| 520 | |a METHODS: Nine S. aureus isolates, including six genetically diverse strains harbouring blaZ, were sent in triplicate to 34 participating laboratories from Australia (n = 14), New Zealand (n = 6), Canada (n = 12), Singapore (n = 1) and Israel (n = 1). We used blaZ PCR as the gold standard to assess susceptibility testing performance of CLSI (P10 disc) and EUCAST (P1 disc) methods. Very major errors (VMEs), major error (MEs) and categorical agreement were calculated | ||
| 520 | |a RESULTS: Twenty-two laboratories reported 593 results according to CLSI methodology (P10 disc). Nineteen laboratories reported 513 results according to the EUCAST (P1 disc) method. For CLSI laboratories, the categorical agreement and calculated VME and ME rates were 85% (508/593), 21% (84/396) and 1.5% (3/198), respectively. For EUCAST laboratories, the categorical agreement and calculated VME and ME rates were 93% (475/513), 11% (84/396) and 1% (3/198), respectively. Seven laboratories reported results for both methods, with VME rates of 24% for CLSI and 12% for EUCAST | ||
| 520 | |a CONCLUSIONS: The EUCAST method with a P1 disc resulted in a lower VME rate compared with the CLSI methods with a P10 disc. These results should be considered in the context that among collections of PSSA isolates, as determined by automated MIC testing, less than 10% harbour blaZ. Furthermore, the clinical relevance of phenotypically susceptible, but blaZ-positive S. aureus, remains unclear | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Anti-Bacterial Agents |2 NLM | |
| 650 | 7 | |a Penicillins |2 NLM | |
| 700 | 1 | |a Cheng, Matthew P |e verfasserin |4 aut | |
| 700 | 1 | |a Chew, Ka Lip |e verfasserin |4 aut | |
| 700 | 1 | |a Coombs, Geoffrey W |e verfasserin |4 aut | |
| 700 | 1 | |a Davis, Joshua S |e verfasserin |4 aut | |
| 700 | 1 | |a Grant, Jennifer M |e verfasserin |4 aut | |
| 700 | 1 | |a Gregson, Dan |e verfasserin |4 aut | |
| 700 | 1 | |a Giulieri, Stefano G |e verfasserin |4 aut | |
| 700 | 1 | |a Howden, Benjamin P |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Todd C |e verfasserin |4 aut | |
| 700 | 1 | |a Nguyen, Vi |e verfasserin |4 aut | |
| 700 | 1 | |a Mora, Jocelyn M |e verfasserin |4 aut | |
| 700 | 1 | |a Morpeth, Susan C |e verfasserin |4 aut | |
| 700 | 1 | |a Robinson, James O |e verfasserin |4 aut | |
| 700 | 1 | |a Tong, Steven Y C |e verfasserin |4 aut | |
| 700 | 1 | |a Van Hal, Sebastiaan J |e verfasserin |4 aut | |
| 700 | 0 | |a Microbiology Working Group of the Staphylococcus aureus Network Adaptive Platform (SNAP) Trial Group |e verfasserin |4 aut | |
| 700 | 1 | |a Abdul-Hameed, Reem |e investigator |4 oth | |
| 700 | 1 | |a Addidle, Michael |e investigator |4 oth | |
| 700 | 1 | |a Athan, Eugene |e investigator |4 oth | |
| 700 | 1 | |a Bloomfield, Max |e investigator |4 oth | |
| 700 | 1 | |a Bond, Katherine |e investigator |4 oth | |
| 700 | 1 | |a Botheras, Carly |e investigator |4 oth | |
| 700 | 1 | |a Bradbury, Susan |e investigator |4 oth | |
| 700 | 1 | |a Carignan, Alex |e investigator |4 oth | |
| 700 | 1 | |a Chan, Wilson |e investigator |4 oth | |
| 700 | 1 | |a Contronei, Rose |e investigator |4 oth | |
| 700 | 1 | |a Cooley, Louise |e investigator |4 oth | |
| 700 | 1 | |a Creighton, Julie |e investigator |4 oth | |
| 700 | 1 | |a Daley, Peter |e investigator |4 oth | |
| 700 | 1 | |a Daneman, Nick |e investigator |4 oth | |
| 700 | 1 | |a Diggle, Matthew |e investigator |4 oth | |
| 700 | 1 | |a Drinković, Dragana |e investigator |4 oth | |
| 700 | 1 | |a Elvy, Juliet |e investigator |4 oth | |
| 700 | 1 | |a Flett, Nadine |e investigator |4 oth | |
| 700 | 1 | |a Foo, Hong |e investigator |4 oth | |
| 700 | 1 | |a Frazer, Jaimie |e investigator |4 oth | |
| 700 | 1 | |a Ghanem-Zoubi, Nesrin |e investigator |4 oth | |
| 700 | 1 | |a Goodman, Anna |e investigator |4 oth | |
| 700 | 1 | |a Gregory, Clair |e investigator |4 oth | |
| 700 | 1 | |a Harkness, Jock |e investigator |4 oth | |
| 700 | 1 | |a Hoddle, Melissa |e investigator |4 oth | |
| 700 | 1 | |a Howard, Julia |e investigator |4 oth | |
| 700 | 1 | |a Jissam, Ali |e investigator |4 oth | |
| 700 | 1 | |a Kalan, Kristin |e investigator |4 oth | |
| 700 | 1 | |a Kalukottege, Pankaja |e investigator |4 oth | |
| 700 | 1 | |a Kelley, Peter |e investigator |4 oth | |
| 700 | 1 | |a Korman, Tony M |e investigator |4 oth | |
| 700 | 1 | |a Kozak, Robert |e investigator |4 oth | |
| 700 | 1 | |a Lagace-Wiens, Philippe |e investigator |4 oth | |
| 700 | 1 | |a Larrotta, Adriana |e investigator |4 oth | |
| 700 | 1 | |a Leong, Queenie |e investigator |4 oth | |
| 700 | 1 | |a Leroi, Marcel |e investigator |4 oth | |
| 700 | 1 | |a Lorenz, David |e investigator |4 oth | |
| 700 | 1 | |a Martin, Philippe |e investigator |4 oth | |
| 700 | 1 | |a Mathew, Susy |e investigator |4 oth | |
| 700 | 1 | |a McEwan, Belinda |e investigator |4 oth | |
| 700 | 1 | |a McGlinchey, Andrew |e investigator |4 oth | |
| 700 | 1 | |a McKew, Genevieve |e investigator |4 oth | |
| 700 | 1 | |a McMullan, Brendan |e investigator |4 oth | |
| 700 | 1 | |a Merlino, John |e investigator |4 oth | |
| 700 | 1 | |a Mrkusich, Angela |e investigator |4 oth | |
| 700 | 1 | |a Munroe, Sean |e investigator |4 oth | |
| 700 | 1 | |a Newton, Peter |e investigator |4 oth | |
| 700 | 1 | |a Parkes, Leighanne O |e investigator |4 oth | |
| 700 | 1 | |a Pollak, Dina |e investigator |4 oth | |
| 700 | 1 | |a Robinson, Murray |e investigator |4 oth | |
| 700 | 1 | |a Roessler, Sharon |e investigator |4 oth | |
| 700 | 1 | |a Russell, Madeline |e investigator |4 oth | |
| 700 | 1 | |a Satie, Maria |e investigator |4 oth | |
| 700 | 1 | |a Sharma, Narinder |e investigator |4 oth | |
| 700 | 1 | |a Sigal, Mendelsohn |e investigator |4 oth | |
| 700 | 1 | |a Streitberg, Richard |e investigator |4 oth | |
| 700 | 1 | |a Tan, Vincent |e investigator |4 oth | |
| 700 | 1 | |a van der Werff, Koen |e investigator |4 oth | |
| 700 | 1 | |a Virey, Evangeline S |e investigator |4 oth | |
| 700 | 1 | |a Wilson, Heather |e investigator |4 oth | |
| 700 | 1 | |a Yamarura, Deb |e investigator |4 oth | |
| 700 | 1 | |a Yu, Yang |e investigator |4 oth | |
| 700 | 1 | |a Ziochos, Helen |e investigator |4 oth | |
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