Next-generation large-scale binary protein interaction network for Drosophila melanogaster
© 2023. The Author(s)..
Generating reference maps of interactome networks illuminates genetic studies by providing a protein-centric approach to finding new components of existing pathways, complexes, and processes. We apply state-of-the-art methods to identify binary protein-protein interactions (PPIs) for Drosophila melanogaster. Four all-by-all yeast two-hybrid (Y2H) screens of > 10,000 Drosophila proteins result in the 'FlyBi' dataset of 8723 PPIs among 2939 proteins. Testing subsets of data from FlyBi and previous PPI studies using an orthogonal assay allows for normalization of data quality; subsequent integration of FlyBi and previous data results in an expanded binary Drosophila reference interaction network, DroRI, comprising 17,232 interactions among 6511 proteins. We use FlyBi data to generate an autophagy network, then validate in vivo using autophagy-related assays. The deformed wings (dwg) gene encodes a protein that is both a regulator and a target of autophagy. Altogether, these resources provide a foundation for building new hypotheses regarding protein networks and function.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:14 |
|---|---|
| Enthalten in: |
Nature communications - 14(2023), 1 vom: 15. Apr., Seite 2162 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Tang, Hong-Wen [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Drosophila Proteins |
|---|
| Anmerkungen: |
Date Completed 18.04.2023 Date Revised 17.11.2023 published: Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1038/s41467-023-37876-0 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM355656639 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM355656639 | ||
| 003 | DE-627 | ||
| 005 | 20231226064815.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/s41467-023-37876-0 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1185.xml |
| 035 | |a (DE-627)NLM355656639 | ||
| 035 | |a (NLM)37061542 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Tang, Hong-Wen |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Next-generation large-scale binary protein interaction network for Drosophila melanogaster |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 18.04.2023 | ||
| 500 | |a Date Revised 17.11.2023 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2023. The Author(s). | ||
| 520 | |a Generating reference maps of interactome networks illuminates genetic studies by providing a protein-centric approach to finding new components of existing pathways, complexes, and processes. We apply state-of-the-art methods to identify binary protein-protein interactions (PPIs) for Drosophila melanogaster. Four all-by-all yeast two-hybrid (Y2H) screens of > 10,000 Drosophila proteins result in the 'FlyBi' dataset of 8723 PPIs among 2939 proteins. Testing subsets of data from FlyBi and previous PPI studies using an orthogonal assay allows for normalization of data quality; subsequent integration of FlyBi and previous data results in an expanded binary Drosophila reference interaction network, DroRI, comprising 17,232 interactions among 6511 proteins. We use FlyBi data to generate an autophagy network, then validate in vivo using autophagy-related assays. The deformed wings (dwg) gene encodes a protein that is both a regulator and a target of autophagy. Altogether, these resources provide a foundation for building new hypotheses regarding protein networks and function | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 7 | |a Drosophila Proteins |2 NLM | |
| 700 | 1 | |a Spirohn, Kerstin |e verfasserin |4 aut | |
| 700 | 1 | |a Hu, Yanhui |e verfasserin |4 aut | |
| 700 | 1 | |a Hao, Tong |e verfasserin |4 aut | |
| 700 | 1 | |a Kovács, István A |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, Yue |e verfasserin |4 aut | |
| 700 | 1 | |a Binari, Richard |e verfasserin |4 aut | |
| 700 | 1 | |a Yang-Zhou, Donghui |e verfasserin |4 aut | |
| 700 | 1 | |a Wan, Kenneth H |e verfasserin |4 aut | |
| 700 | 1 | |a Bader, Joel S |e verfasserin |4 aut | |
| 700 | 1 | |a Balcha, Dawit |e verfasserin |4 aut | |
| 700 | 1 | |a Bian, Wenting |e verfasserin |4 aut | |
| 700 | 1 | |a Booth, Benjamin W |e verfasserin |4 aut | |
| 700 | 1 | |a Coté, Atina G |e verfasserin |4 aut | |
| 700 | 1 | |a de Rouck, Steffi |e verfasserin |4 aut | |
| 700 | 1 | |a Desbuleux, Alice |e verfasserin |4 aut | |
| 700 | 1 | |a Goh, Kah Yong |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Dae-Kyum |e verfasserin |4 aut | |
| 700 | 1 | |a Knapp, Jennifer J |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Wen Xing |e verfasserin |4 aut | |
| 700 | 1 | |a Lemmens, Irma |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Cathleen |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Mian |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Roujia |e verfasserin |4 aut | |
| 700 | 1 | |a Lim, Hyobin Julianne |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Yifang |e verfasserin |4 aut | |
| 700 | 1 | |a Luck, Katja |e verfasserin |4 aut | |
| 700 | 1 | |a Markey, Dylan |e verfasserin |4 aut | |
| 700 | 1 | |a Pollis, Carl |e verfasserin |4 aut | |
| 700 | 1 | |a Rangarajan, Sudharshan |e verfasserin |4 aut | |
| 700 | 1 | |a Rodiger, Jonathan |e verfasserin |4 aut | |
| 700 | 1 | |a Schlabach, Sadie |e verfasserin |4 aut | |
| 700 | 1 | |a Shen, Yun |e verfasserin |4 aut | |
| 700 | 1 | |a Sheykhkarimli, Dayag |e verfasserin |4 aut | |
| 700 | 1 | |a TeeKing, Bridget |e verfasserin |4 aut | |
| 700 | 1 | |a Roth, Frederick P |e verfasserin |4 aut | |
| 700 | 1 | |a Tavernier, Jan |e verfasserin |4 aut | |
| 700 | 1 | |a Calderwood, Michael A |e verfasserin |4 aut | |
| 700 | 1 | |a Hill, David E |e verfasserin |4 aut | |
| 700 | 1 | |a Celniker, Susan E |e verfasserin |4 aut | |
| 700 | 1 | |a Vidal, Marc |e verfasserin |4 aut | |
| 700 | 1 | |a Perrimon, Norbert |e verfasserin |4 aut | |
| 700 | 1 | |a Mohr, Stephanie E |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Nature communications |d 2010 |g 14(2023), 1 vom: 15. Apr., Seite 2162 |w (DE-627)NLM199274525 |x 2041-1723 |7 nnns |
| 773 | 1 | 8 | |g volume:14 |g year:2023 |g number:1 |g day:15 |g month:04 |g pages:2162 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1038/s41467-023-37876-0 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 14 |j 2023 |e 1 |b 15 |c 04 |h 2162 | ||