Native doublet microtubules from Tetrahymena thermophila reveal the importance of outer junction proteins
© 2023. The Author(s)..
Cilia are ubiquitous eukaryotic organelles responsible for cellular motility and sensory functions. The ciliary axoneme is a microtubule-based cytoskeleton consisting of two central singlets and nine outer doublet microtubules. Cryo-electron microscopy-based studies have revealed a complex network inside the lumen of both tubules composed of microtubule-inner proteins (MIPs). However, the functions of most MIPs remain unknown. Here, we present single-particle cryo-EM-based analyses of the Tetrahymena thermophila native doublet microtubule and identify 42 MIPs. These data shed light on the evolutionarily conserved and diversified roles of MIPs. In addition, we identified MIPs potentially responsible for the assembly and stability of the doublet outer junction. Knockout of the evolutionarily conserved outer junction component CFAP77 moderately diminishes Tetrahymena swimming speed and beat frequency, indicating the important role of CFAP77 and outer junction stability in cilia beating generation and/or regulation.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:14 |
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| Enthalten in: |
Nature communications - 14(2023), 1 vom: 15. Apr., Seite 2168 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kubo, Shintaroh [VerfasserIn] |
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| Links: |
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| Themen: |
Journal Article |
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| Anmerkungen: |
Date Completed 18.04.2023 Date Revised 02.12.2023 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s41467-023-37868-0 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM355656582 |
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| 245 | 1 | 0 | |a Native doublet microtubules from Tetrahymena thermophila reveal the importance of outer junction proteins |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2023. The Author(s). | ||
| 520 | |a Cilia are ubiquitous eukaryotic organelles responsible for cellular motility and sensory functions. The ciliary axoneme is a microtubule-based cytoskeleton consisting of two central singlets and nine outer doublet microtubules. Cryo-electron microscopy-based studies have revealed a complex network inside the lumen of both tubules composed of microtubule-inner proteins (MIPs). However, the functions of most MIPs remain unknown. Here, we present single-particle cryo-EM-based analyses of the Tetrahymena thermophila native doublet microtubule and identify 42 MIPs. These data shed light on the evolutionarily conserved and diversified roles of MIPs. In addition, we identified MIPs potentially responsible for the assembly and stability of the doublet outer junction. Knockout of the evolutionarily conserved outer junction component CFAP77 moderately diminishes Tetrahymena swimming speed and beat frequency, indicating the important role of CFAP77 and outer junction stability in cilia beating generation and/or regulation | ||
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| 700 | 1 | |a Black, Corbin S |e verfasserin |4 aut | |
| 700 | 1 | |a Joachimiak, Ewa |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Shun Kai |e verfasserin |4 aut | |
| 700 | 1 | |a Legal, Thibault |e verfasserin |4 aut | |
| 700 | 1 | |a Peri, Katya |e verfasserin |4 aut | |
| 700 | 1 | |a Khalifa, Ahmad Abdelzaher Zaki |e verfasserin |4 aut | |
| 700 | 1 | |a Ghanaeian, Avrin |e verfasserin |4 aut | |
| 700 | 1 | |a McCafferty, Caitlyn L |e verfasserin |4 aut | |
| 700 | 1 | |a Valente-Paterno, Melissa |e verfasserin |4 aut | |
| 700 | 1 | |a De Bellis, Chelsea |e verfasserin |4 aut | |
| 700 | 1 | |a Huynh, Phuong M |e verfasserin |4 aut | |
| 700 | 1 | |a Fan, Zhe |e verfasserin |4 aut | |
| 700 | 1 | |a Marcotte, Edward M |e verfasserin |4 aut | |
| 700 | 1 | |a Wloga, Dorota |e verfasserin |4 aut | |
| 700 | 1 | |a Bui, Khanh Huy |e verfasserin |4 aut | |
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