Detection of viral antibodies in camel sera using magnetic particle spectroscopy
© 2023. The Author(s)..
Pandemics like SARS-Cov-2 very frequently have their origin in different animals and in particular herds of camels could be a source of zoonotic diseases. This study took advantage on a highly sensitive and adaptable method for the fast and reliable detection of viral antibodies in camels using low-cost equipment. Magnetic nanoparticles (MNP) have high variability in their functionalization with different peptides and proteins. We confirm that 3-aminopropyl triethoxysilane (APTES)-coated MNP could be functionalized with viral proteins. The protein loading could be confirmed by simple loading controls using FACS-analysis (p < 0.05). Complementary combination of antigen and antibody yields in a significant signal increase could be proven by both FACS and COMPASS. However, COMPASS needs only a few seconds for the measurement. In COMPASS, the phase φn on selected critical point of the fifth higher harmonic (n = 5th). Here, positive sera display highly significant signal increase over the control or negative sera. Furthermore, a clear distinction could be made in antibody detection as an immune response to closely related viruses (SARS-CoV2 and MERS). Using modified MNPs along with COMPASS offers a fast and reliable method that is less cost intensive than current technologies and offers the possibility to be quickly adapted in case of new occurring viral infections. KEY POINTS: • COMPASS (critical offset magnetic particle spectroscopy) allows the fast detection of antibodies. • Magnetic nanoparticles can be adapted by exchange of the linked bait molecule. • Antibodies could be detected in camel sera without washing steps within seconds.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:107 |
|---|---|
| Enthalten in: |
Applied microbiology and biotechnology - 107(2023), 10 vom: 15. Mai, Seite 3329-3339 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Friedrich, Bernhard [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Antibodies, Viral |
|---|
| Anmerkungen: |
Date Completed 08.05.2023 Date Revised 08.05.2023 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1007/s00253-023-12513-4 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM355645904 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM355645904 | ||
| 003 | DE-627 | ||
| 005 | 20231226064801.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s00253-023-12513-4 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1185.xml |
| 035 | |a (DE-627)NLM355645904 | ||
| 035 | |a (NLM)37060465 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Friedrich, Bernhard |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Detection of viral antibodies in camel sera using magnetic particle spectroscopy |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 08.05.2023 | ||
| 500 | |a Date Revised 08.05.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2023. The Author(s). | ||
| 520 | |a Pandemics like SARS-Cov-2 very frequently have their origin in different animals and in particular herds of camels could be a source of zoonotic diseases. This study took advantage on a highly sensitive and adaptable method for the fast and reliable detection of viral antibodies in camels using low-cost equipment. Magnetic nanoparticles (MNP) have high variability in their functionalization with different peptides and proteins. We confirm that 3-aminopropyl triethoxysilane (APTES)-coated MNP could be functionalized with viral proteins. The protein loading could be confirmed by simple loading controls using FACS-analysis (p < 0.05). Complementary combination of antigen and antibody yields in a significant signal increase could be proven by both FACS and COMPASS. However, COMPASS needs only a few seconds for the measurement. In COMPASS, the phase φn on selected critical point of the fifth higher harmonic (n = 5th). Here, positive sera display highly significant signal increase over the control or negative sera. Furthermore, a clear distinction could be made in antibody detection as an immune response to closely related viruses (SARS-CoV2 and MERS). Using modified MNPs along with COMPASS offers a fast and reliable method that is less cost intensive than current technologies and offers the possibility to be quickly adapted in case of new occurring viral infections. KEY POINTS: • COMPASS (critical offset magnetic particle spectroscopy) allows the fast detection of antibodies. • Magnetic nanoparticles can be adapted by exchange of the linked bait molecule. • Antibodies could be detected in camel sera without washing steps within seconds | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Antibody detection | |
| 650 | 4 | |a COMPASS | |
| 650 | 4 | |a Camels | |
| 650 | 4 | |a MNPs | |
| 650 | 4 | |a MPS | |
| 650 | 4 | |a Rapid bioassay | |
| 650 | 4 | |a SARS-Cov-2 | |
| 650 | 7 | |a Antibodies, Viral |2 NLM | |
| 650 | 7 | |a RNA, Viral |2 NLM | |
| 700 | 1 | |a Vogel, Patrick |e verfasserin |4 aut | |
| 700 | 1 | |a Rückert, Martin A |e verfasserin |4 aut | |
| 700 | 1 | |a Lyer, Stefan |e verfasserin |4 aut | |
| 700 | 1 | |a Günther, Johanna |e verfasserin |4 aut | |
| 700 | 1 | |a Wernery, Ulrich |e verfasserin |4 aut | |
| 700 | 1 | |a Joseph, Sunitha |e verfasserin |4 aut | |
| 700 | 1 | |a Müller, Judith |e verfasserin |4 aut | |
| 700 | 1 | |a Behr, Volker C |e verfasserin |4 aut | |
| 700 | 1 | |a Alexiou, Christoph |e verfasserin |4 aut | |
| 700 | 1 | |a Tietze, Rainer |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Applied microbiology and biotechnology |d 1991 |g 107(2023), 10 vom: 15. Mai, Seite 3329-3339 |w (DE-627)NLM01261680X |x 1432-0614 |7 nnns |
| 773 | 1 | 8 | |g volume:107 |g year:2023 |g number:10 |g day:15 |g month:05 |g pages:3329-3339 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/s00253-023-12513-4 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 107 |j 2023 |e 10 |b 15 |c 05 |h 3329-3339 | ||