Toll-Like Receptor 7 a Novel Non-Invasive Inflammatory Genetic Sensor for Ulcerative Colitis Remission Monitoring
Copyright: © 2023 Advanced Biomedical Research..
Background: Ulcerative colitis (UC) and Crohn's disease (CD) are two major types of inflammatory bowel diseases (IBDs). Toll-like receptors (TLRs) are expressed in the innate immune system compartments, in charge of identifying a wide range of microorganisms. The aim of the present study was to evaluate the expression of TLR-2, -7, and -8 in peripheral blood mononuclear cells (PBMC) of UC patients as a novel non-invasive primary inflammation sensor for monitoring the clinical course of UC candidates.
Materials and Methods: In this cross-sectional study, total RNA was extracted from the PBMC of 42 UC patients along with 20 healthy donors. The mRNA levels of TLR-2, -7, and -8 were assessed using the quantitative real-time polymerase chain (qRT-PCR) reaction.
Results: The present research study demonstrated no significant changes in TLR-2 mRNA expression in UC patients in comparison with the control group (P = 0.1264), whereas significant elevation (P = 0.0008) was distinguished in the TLR-7 expression of UC participants specifically during the remission course compared with healthy donors and flareup patients (P = 0.0004 and P = 0.0063, respectively). The last selected TLR, TLR-8 was not shown remarkable changes either between UC patients and the control group or between clinical courses of the disease.
Conclusion: Here, among three nominated TLRs for predicting UC patients, TLR-7 was potentially selected according to the significant difference in mRNA expression in flareup UC patients and control donors. TLR-7 could be used as a novel non-invasive biomarker for monitoring UC patients in the active course of the disease.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:12 |
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| Enthalten in: |
Advanced biomedical research - 12(2023) vom: 05., Seite 54 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Asadzadeh-Aghdaei, Hamid [VerfasserIn] |
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| Links: |
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| Themen: |
Biomarkers |
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| Anmerkungen: |
Date Revised 15.04.2023 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.4103/abr.abr_24_22 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM355615746 |
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| 520 | |a Copyright: © 2023 Advanced Biomedical Research. | ||
| 520 | |a Background: Ulcerative colitis (UC) and Crohn's disease (CD) are two major types of inflammatory bowel diseases (IBDs). Toll-like receptors (TLRs) are expressed in the innate immune system compartments, in charge of identifying a wide range of microorganisms. The aim of the present study was to evaluate the expression of TLR-2, -7, and -8 in peripheral blood mononuclear cells (PBMC) of UC patients as a novel non-invasive primary inflammation sensor for monitoring the clinical course of UC candidates | ||
| 520 | |a Materials and Methods: In this cross-sectional study, total RNA was extracted from the PBMC of 42 UC patients along with 20 healthy donors. The mRNA levels of TLR-2, -7, and -8 were assessed using the quantitative real-time polymerase chain (qRT-PCR) reaction | ||
| 520 | |a Results: The present research study demonstrated no significant changes in TLR-2 mRNA expression in UC patients in comparison with the control group (P = 0.1264), whereas significant elevation (P = 0.0008) was distinguished in the TLR-7 expression of UC participants specifically during the remission course compared with healthy donors and flareup patients (P = 0.0004 and P = 0.0063, respectively). The last selected TLR, TLR-8 was not shown remarkable changes either between UC patients and the control group or between clinical courses of the disease | ||
| 520 | |a Conclusion: Here, among three nominated TLRs for predicting UC patients, TLR-7 was potentially selected according to the significant difference in mRNA expression in flareup UC patients and control donors. TLR-7 could be used as a novel non-invasive biomarker for monitoring UC patients in the active course of the disease | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Biomarkers | |
| 650 | 4 | |a colitis | |
| 650 | 4 | |a inflammatory bowel disease | |
| 650 | 4 | |a remission | |
| 650 | 4 | |a toll-like receptor 7 | |
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| 700 | 1 | |a Zamani, Naghmeh |e verfasserin |4 aut | |
| 700 | 1 | |a Baradaran-Ghavami, Shaghayegh |e verfasserin |4 aut | |
| 700 | 1 | |a Nemati, Mohsen |e verfasserin |4 aut | |
| 700 | 1 | |a Shahrokh, Shabnam |e verfasserin |4 aut | |
| 700 | 1 | |a Norouzinia, Mohsen |e verfasserin |4 aut | |
| 700 | 1 | |a Vosough, Massoud |e verfasserin |4 aut | |
| 700 | 1 | |a Nazemalhosseini-Mojarad, Ehsan |e verfasserin |4 aut | |
| 700 | 1 | |a Zali, Mohammadreza |e verfasserin |4 aut | |
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