COX10-AS1-mediated miR-361-5p regulated cell invasion and migration by targeting SPRY1 in oral squamous cell carcinoma
AJTR Copyright © 2023..
BACKGROUND: COX10-AS1 belongs to the class of lncRNA and has been shown to influence carcinogenesis; however, its function and underlying mechanism in oral squamous cell carcinoma are still unclear (OSCC).
METHOD: Western blotting, immunohistochemistry, and RT-PCR were used to identify gene expression. Cell invasion and migration were discovered using Transwell and Scratch-Wound analyses. The interaction between lncRNA and miRNA was examined using dual-luciferase and immunofluorescence assays.
RESULTS: We discovered that COX10-AS1 was significantly downregulated in OSCC tissues when compared to matched noncancerous tissues, indicating a dismal prognosis for OSCC patients. By raising the expression of MMP-2/-9 and Snail and lowering the expression of E-cadherin, COX10-AS1 deletion increased OSCC cell invasion and migration. Next, three binding sites between COX10-AS1 and miR-361-5p were shown in the StarBase V2.0 database. Pearson's correlation analysis revealed a negative association between the expression of COX10-AS1 and that of miR-361-5p, and miR-361-5p transfection reduced COX10-AS1's influence on OSCC cell invasion and migration. Furthermore, COX10-AS1 favorably regulated SPRY1, a miR-361-5p target gene.
CONCLUSION: Through the miR-361-5p/SPRY1 axis, COX10-AS1 can act as a tumor suppressor and is decreased in OSCC.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:15 |
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| Enthalten in: |
American journal of translational research - 15(2023), 3 vom: 18., Seite 2191-2206 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Deng, Jing [VerfasserIn] |
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| Themen: |
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| Anmerkungen: |
Date Revised 15.04.2023 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM355611562 |
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| 100 | 1 | |a Deng, Jing |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a COX10-AS1-mediated miR-361-5p regulated cell invasion and migration by targeting SPRY1 in oral squamous cell carcinoma |
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| 500 | |a Date Revised 15.04.2023 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a AJTR Copyright © 2023. | ||
| 520 | |a BACKGROUND: COX10-AS1 belongs to the class of lncRNA and has been shown to influence carcinogenesis; however, its function and underlying mechanism in oral squamous cell carcinoma are still unclear (OSCC) | ||
| 520 | |a METHOD: Western blotting, immunohistochemistry, and RT-PCR were used to identify gene expression. Cell invasion and migration were discovered using Transwell and Scratch-Wound analyses. The interaction between lncRNA and miRNA was examined using dual-luciferase and immunofluorescence assays | ||
| 520 | |a RESULTS: We discovered that COX10-AS1 was significantly downregulated in OSCC tissues when compared to matched noncancerous tissues, indicating a dismal prognosis for OSCC patients. By raising the expression of MMP-2/-9 and Snail and lowering the expression of E-cadherin, COX10-AS1 deletion increased OSCC cell invasion and migration. Next, three binding sites between COX10-AS1 and miR-361-5p were shown in the StarBase V2.0 database. Pearson's correlation analysis revealed a negative association between the expression of COX10-AS1 and that of miR-361-5p, and miR-361-5p transfection reduced COX10-AS1's influence on OSCC cell invasion and migration. Furthermore, COX10-AS1 favorably regulated SPRY1, a miR-361-5p target gene | ||
| 520 | |a CONCLUSION: Through the miR-361-5p/SPRY1 axis, COX10-AS1 can act as a tumor suppressor and is decreased in OSCC | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a COX10-AS1 | |
| 650 | 4 | |a OSCC | |
| 650 | 4 | |a invasion | |
| 650 | 4 | |a miR-361-5p | |
| 650 | 4 | |a migration | |
| 700 | 1 | |a Wu, Maolin |e verfasserin |4 aut | |
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