A dual "turn-on" biosensor based on AIE effect and FRET for in situ detection of miR-125b biomarker in early Alzheimer's disease
Copyright © 2023 Elsevier B.V. All rights reserved..
MicroRNA-125b (miR-125b) is highly associated with synaptic dysfunction and tau hyperphosphorylation in the early pathogenesis of Alzheimer's disease (AD), making it a promising biomarker for early AD diagnosis. Hence, there is an urgent need for a reliable sensing platform to assist in situ miR-125b detection. In this work, we report a dual "turn-on" fluorescence biosensor based on the nanocomposite of aggregation-induced emission fluorogen (AIEgen)-labeled oligonucleotide (TPET-DNA) probes immobilized on the surface of cationic dextran modified molybdenum disulfide (TPET-DNADex-MoS2). In the presence of the target, TEPT-DNA can hybridize with miR-125b to form a DNA/RNA duplex, causing TPET-DNA to detach from the surface of Dex-MoS2 that simultaneously activates the dual fluorescence enhancement processes: (1) recovery of TPET-DNA signal and (2) strong fluorescent emission from AIEgen triggered by restriction of the intramolecular rotation. The sensing performance of TPET-DNA@Dex-MoS2 was demonstrated by detecting miR-125b in vitro with good sensitivity at the picomolar level and rapid response (≤1 h) without amplification procedures. Furthermore, our nanoprobes exhibited excellent imaging capabilities to aid real-time monitoring of the endogenous miR-125b in PC12 cells and brain tissues of mice AD model induced by local administration of okadaic acid (OA). The fluorescence signals of the nanoprobes indicated miR-125b was spatially associated with phosphorylated tau protein (p-tau) in vitro and in vivo. Therefore, TPET-DNA@Dex-MoS2 could be a promising tool for in situ and real-time monitoring of the AD-related microRNAs and also provide mechanistic insight into the early prognosis of AD.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2023 |
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Erschienen: |
2023 |
Enthalten in: |
Zur Gesamtaufnahme - volume:230 |
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Enthalten in: |
Biosensors & bioelectronics - 230(2023) vom: 15. Juni, Seite 115270 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Zhang, Qin [VerfasserIn] |
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Links: |
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Themen: |
81AH48963U |
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Anmerkungen: |
Date Completed 17.04.2023 Date Revised 17.04.2023 published: Print-Electronic Citation Status MEDLINE |
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doi: |
10.1016/j.bios.2023.115270 |
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funding: |
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PPN (Katalog-ID): |
NLM355282208 |
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100 | 1 | |a Zhang, Qin |e verfasserin |4 aut | |
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520 | |a MicroRNA-125b (miR-125b) is highly associated with synaptic dysfunction and tau hyperphosphorylation in the early pathogenesis of Alzheimer's disease (AD), making it a promising biomarker for early AD diagnosis. Hence, there is an urgent need for a reliable sensing platform to assist in situ miR-125b detection. In this work, we report a dual "turn-on" fluorescence biosensor based on the nanocomposite of aggregation-induced emission fluorogen (AIEgen)-labeled oligonucleotide (TPET-DNA) probes immobilized on the surface of cationic dextran modified molybdenum disulfide (TPET-DNADex-MoS2). In the presence of the target, TEPT-DNA can hybridize with miR-125b to form a DNA/RNA duplex, causing TPET-DNA to detach from the surface of Dex-MoS2 that simultaneously activates the dual fluorescence enhancement processes: (1) recovery of TPET-DNA signal and (2) strong fluorescent emission from AIEgen triggered by restriction of the intramolecular rotation. The sensing performance of TPET-DNA@Dex-MoS2 was demonstrated by detecting miR-125b in vitro with good sensitivity at the picomolar level and rapid response (≤1 h) without amplification procedures. Furthermore, our nanoprobes exhibited excellent imaging capabilities to aid real-time monitoring of the endogenous miR-125b in PC12 cells and brain tissues of mice AD model induced by local administration of okadaic acid (OA). The fluorescence signals of the nanoprobes indicated miR-125b was spatially associated with phosphorylated tau protein (p-tau) in vitro and in vivo. Therefore, TPET-DNA@Dex-MoS2 could be a promising tool for in situ and real-time monitoring of the AD-related microRNAs and also provide mechanistic insight into the early prognosis of AD | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Aggregation-induced emission (AIE) fluorogens | |
650 | 4 | |a Alzheimer's disease | |
650 | 4 | |a Molybdenum disulfide (MoS(2)) | |
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700 | 1 | |a Yin, Bohan |e verfasserin |4 aut | |
700 | 1 | |a Huang, Yingying |e verfasserin |4 aut | |
700 | 1 | |a Gu, Yutian |e verfasserin |4 aut | |
700 | 1 | |a Yan, Jiaxiang |e verfasserin |4 aut | |
700 | 1 | |a Chen, Jiareng |e verfasserin |4 aut | |
700 | 1 | |a Li, Chuanqi |e verfasserin |4 aut | |
700 | 1 | |a Zhang, Yu |e verfasserin |4 aut | |
700 | 1 | |a Wong, Siu Hong Dexter |e verfasserin |4 aut | |
700 | 1 | |a Yang, Mo |e verfasserin |4 aut | |
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