Wnt/β-catenin signaling induces axial elasticity patterns of Hydra extracellular matrix
© 2023 The Authors..
The extracellular matrix (ECM) plays crucial roles in animal development and diseases. Here, we report that Wnt/β-catenin signaling induces the ECM remodeling during Hydra axis formation. We determined the micro- and nanoscopic arrangement of fibrillar type I collagen along Hydra's body axis using high-resolution microscopy and X-ray scattering. Elasticity mapping of the ECM ex vivo revealed distinctive elasticity patterns along the body axis. A proteomic analysis of the ECM showed that these elasticity patterns correlate with a gradient-like distribution of metalloproteases along the body axis. Activation of the Wnt/β-catenin pathway in wild-type and transgenic animals alters these patterns toward low ECM elasticity patterns. This suggests a mechanism whereby high protease activity under control of Wnt/β-catenin signaling causes remodeling and softening of the ECM. This Wnt-dependent spatiotemporal coordination of biochemical and biomechanical cues in ECM formation was likely a central evolutionary innovation for animal tissue morphogenesis.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:26 |
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| Enthalten in: |
iScience - 26(2023), 4 vom: 21. Apr., Seite 106416 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Veschgini, Mariam [VerfasserIn] |
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| Links: |
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| Themen: |
Histology |
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| Anmerkungen: |
Date Revised 04.04.2023 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1016/j.isci.2023.106416 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM355140608 |
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| 520 | |a The extracellular matrix (ECM) plays crucial roles in animal development and diseases. Here, we report that Wnt/β-catenin signaling induces the ECM remodeling during Hydra axis formation. We determined the micro- and nanoscopic arrangement of fibrillar type I collagen along Hydra's body axis using high-resolution microscopy and X-ray scattering. Elasticity mapping of the ECM ex vivo revealed distinctive elasticity patterns along the body axis. A proteomic analysis of the ECM showed that these elasticity patterns correlate with a gradient-like distribution of metalloproteases along the body axis. Activation of the Wnt/β-catenin pathway in wild-type and transgenic animals alters these patterns toward low ECM elasticity patterns. This suggests a mechanism whereby high protease activity under control of Wnt/β-catenin signaling causes remodeling and softening of the ECM. This Wnt-dependent spatiotemporal coordination of biochemical and biomechanical cues in ECM formation was likely a central evolutionary innovation for animal tissue morphogenesis | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Histology | |
| 650 | 4 | |a Microscopic anatomy | |
| 650 | 4 | |a Molecular biology | |
| 650 | 4 | |a Zoology | |
| 700 | 1 | |a Suzuki, Ryo |e verfasserin |4 aut | |
| 700 | 1 | |a Kling, Svenja |e verfasserin |4 aut | |
| 700 | 1 | |a Petersen, Hendrik O |e verfasserin |4 aut | |
| 700 | 1 | |a Bergheim, Bruno Gideon |e verfasserin |4 aut | |
| 700 | 1 | |a Abuillan, Wasim |e verfasserin |4 aut | |
| 700 | 1 | |a Linke, Philipp |e verfasserin |4 aut | |
| 700 | 1 | |a Kaufmann, Stefan |e verfasserin |4 aut | |
| 700 | 1 | |a Burghammer, Manfred |e verfasserin |4 aut | |
| 700 | 1 | |a Engel, Ulrike |e verfasserin |4 aut | |
| 700 | 1 | |a Stein, Frank |e verfasserin |4 aut | |
| 700 | 1 | |a Özbek, Suat |e verfasserin |4 aut | |
| 700 | 1 | |a Holstein, Thomas W |e verfasserin |4 aut | |
| 700 | 1 | |a Tanaka, Motomu |e verfasserin |4 aut | |
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