At-home sampling to meet geographical challenges for serological assessment of SARS-CoV-2 exposure in a rural region of northern Sweden, March to May 2021 : a retrospective cohort study
BackgroundThe current SARS-CoV-2 pandemic has highlighted a need for easy and safe blood sampling in combination with accurate serological methodology. Venipuncture for testing is usually performed by trained staff at healthcare centres. Long travel distances to healthcare centres in rural regions may introduce a bias of testing towards relatively large communities with closer access. Rural regions are therefore often not represented in population-based data.AimThe aim of this retrospective cohort study was to develop and implement a strategy for at-home testing in a rural region of Sweden during spring 2021, and to evaluate its role to provide equal health care for its inhabitants.MethodsWe developed a sensitive method to measure antibodies to the S-protein of SARS-CoV-2 and optimised this assay for clinical use together with a strategy of at-home capillary blood sampling.ResultsWe demonstrated that our ELISA gave comparable results after analysis of capillary blood or serum from SARS-CoV-2-experienced individuals. We demonstrated stability of the assay under conditions that reflected temperature and humidity during winter or summer. By assessment of capillary blood samples from 4,122 individuals, we could show both feasibility of the strategy and that implementation shifted the geographical spread of testing in favour of rural areas.ConclusionImplementation of at-home sampling enabled citizens living in remote rural areas access to centralised and sensitive laboratory antibody tests. The strategy for testing used here could therefore enable disease control authorities to get rapid access to information concerning immunity to infectious diseases, even across vast geographical distance.
| Errataetall: | |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:28 |
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| Enthalten in: |
Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin - 28(2023), 13 vom: 11. März |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Byström, Julia Wigren [VerfasserIn] |
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| Links: |
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| Themen: |
Antibodies, Viral |
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| Anmerkungen: |
Date Completed 03.04.2023 Date Revised 04.05.2023 published: Print UpdateOf: medRxiv. 2022 May 18;:. - PMID 32577692 Citation Status MEDLINE |
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| doi: |
10.2807/1560-7917.ES.2023.28.13.2200432 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM355003007 |
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| 100 | 1 | |a Byström, Julia Wigren |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a At-home sampling to meet geographical challenges for serological assessment of SARS-CoV-2 exposure in a rural region of northern Sweden, March to May 2021 |b a retrospective cohort study |
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| 500 | |a published: Print | ||
| 500 | |a UpdateOf: medRxiv. 2022 May 18;:. - PMID 32577692 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a BackgroundThe current SARS-CoV-2 pandemic has highlighted a need for easy and safe blood sampling in combination with accurate serological methodology. Venipuncture for testing is usually performed by trained staff at healthcare centres. Long travel distances to healthcare centres in rural regions may introduce a bias of testing towards relatively large communities with closer access. Rural regions are therefore often not represented in population-based data.AimThe aim of this retrospective cohort study was to develop and implement a strategy for at-home testing in a rural region of Sweden during spring 2021, and to evaluate its role to provide equal health care for its inhabitants.MethodsWe developed a sensitive method to measure antibodies to the S-protein of SARS-CoV-2 and optimised this assay for clinical use together with a strategy of at-home capillary blood sampling.ResultsWe demonstrated that our ELISA gave comparable results after analysis of capillary blood or serum from SARS-CoV-2-experienced individuals. We demonstrated stability of the assay under conditions that reflected temperature and humidity during winter or summer. By assessment of capillary blood samples from 4,122 individuals, we could show both feasibility of the strategy and that implementation shifted the geographical spread of testing in favour of rural areas.ConclusionImplementation of at-home sampling enabled citizens living in remote rural areas access to centralised and sensitive laboratory antibody tests. The strategy for testing used here could therefore enable disease control authorities to get rapid access to information concerning immunity to infectious diseases, even across vast geographical distance | ||
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| 700 | 1 | |a Rosendal, Ebba |e verfasserin |4 aut | |
| 700 | 1 | |a Gröning, Remigius |e verfasserin |4 aut | |
| 700 | 1 | |a Gwon, Yong-Dae |e verfasserin |4 aut | |
| 700 | 1 | |a Nilsson, Emma |e verfasserin |4 aut | |
| 700 | 1 | |a Sharma, Atin |e verfasserin |4 aut | |
| 700 | 1 | |a Espaillat, Akbar |e verfasserin |4 aut | |
| 700 | 1 | |a Hanke, Leo |e verfasserin |4 aut | |
| 700 | 1 | |a McInerney, Gerald |e verfasserin |4 aut | |
| 700 | 1 | |a Puhar, Andrea |e verfasserin |4 aut | |
| 700 | 1 | |a Cava, Felipe |e verfasserin |4 aut | |
| 700 | 1 | |a Karlsson Hedestam, Gunilla B |e verfasserin |4 aut | |
| 700 | 1 | |a Thunberg, Therese |e verfasserin |4 aut | |
| 700 | 1 | |a Monsen, Tor |e verfasserin |4 aut | |
| 700 | 1 | |a Elgh, Fredrik |e verfasserin |4 aut | |
| 700 | 1 | |a Evander, Magnus |e verfasserin |4 aut | |
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| 700 | 1 | |a Normark, Johan |e verfasserin |4 aut | |
| 700 | 1 | |a Forsell, Mattias Ne |e verfasserin |4 aut | |
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