Multidrug-loaded liposomes prevent ischemic stroke through intranasal administration
Copyright © 2023 The Authors. Published by Elsevier Masson SAS.. All rights reserved..
Baicalin (BA), a multi-target neuroprotective agent, has poor solubility resulting in low bioavailability. In this study, multidrug-loaded liposomes were prepared by encapsulating BA, borneol (BO) and cholic acid (CA) to prevent ischemic stroke. BBC-LP were administered intranasally (i.n.) to deliver into the brain for neuroprotection. Finally, potential mechanism of BBC treating ischemic stroke (IS) was explored by network pharmacology. In this study, BBC-LP was prepared by reverse evaporation method, and the encapsulation efficiency (EE) of the optimized liposomes was 42.69% and the drug loading (DL) was 6.17%. The liposomes had low mean particle size (156.62 ± 2.96 nm), polydispersity index (PDI) (0.195) and zeta potential (-0.99 mv). Compared to BBC, pharmacodynamic studies revealed that BBC-LP significantly improved neurological deficits, brain infarct volume, and cerebral pathology in MCAO rats. Toxicity studies showed that BBC-LP was not irritating to the nasal mucosa. These results suggest that BBC-LP can safely and effectively ameliorate IS injury by i.n. administration. Moreover, it's neuroprotective function may be related to the anti-apoptotic and anti-inflammatory effects exerted by phosphatidylinositol-3-kinase (PI3K)/Akt signaling pathway and mitogen-activated protein kinase (MAPK) signaling pathway.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:162 |
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| Enthalten in: |
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie - 162(2023) vom: 01. Juni, Seite 114542 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yu, Shuang [VerfasserIn] |
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| Links: |
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| Themen: |
Baicalin |
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| Anmerkungen: |
Date Completed 03.05.2023 Date Revised 03.05.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.biopha.2023.114542 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Baicalin (BA), a multi-target neuroprotective agent, has poor solubility resulting in low bioavailability. In this study, multidrug-loaded liposomes were prepared by encapsulating BA, borneol (BO) and cholic acid (CA) to prevent ischemic stroke. BBC-LP were administered intranasally (i.n.) to deliver into the brain for neuroprotection. Finally, potential mechanism of BBC treating ischemic stroke (IS) was explored by network pharmacology. In this study, BBC-LP was prepared by reverse evaporation method, and the encapsulation efficiency (EE) of the optimized liposomes was 42.69% and the drug loading (DL) was 6.17%. The liposomes had low mean particle size (156.62 ± 2.96 nm), polydispersity index (PDI) (0.195) and zeta potential (-0.99 mv). Compared to BBC, pharmacodynamic studies revealed that BBC-LP significantly improved neurological deficits, brain infarct volume, and cerebral pathology in MCAO rats. Toxicity studies showed that BBC-LP was not irritating to the nasal mucosa. These results suggest that BBC-LP can safely and effectively ameliorate IS injury by i.n. administration. Moreover, it's neuroprotective function may be related to the anti-apoptotic and anti-inflammatory effects exerted by phosphatidylinositol-3-kinase (PI3K)/Akt signaling pathway and mitogen-activated protein kinase (MAPK) signaling pathway | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Baicalin | |
| 650 | 4 | |a Intranasal administration | |
| 650 | 4 | |a Ischemic stroke | |
| 650 | 4 | |a Liposome | |
| 650 | 4 | |a Network pharmacology | |
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| 650 | 7 | |a Neuroprotective Agents |2 NLM | |
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| 700 | 1 | |a Shi, Ai |e verfasserin |4 aut | |
| 700 | 1 | |a Long, Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Deng, Jie |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Yin |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Xiaoqiu |e verfasserin |4 aut | |
| 700 | 1 | |a Wen, Jing |e verfasserin |4 aut | |
| 700 | 1 | |a Hu, Yue |e verfasserin |4 aut | |
| 700 | 1 | |a He, Xiaofang |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Yuanyuan |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Nan |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Mengnan |e verfasserin |4 aut | |
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