Low Prevalence of Plasmodium falciparum Histidine-Rich Protein 2 and 3 Gene Deletions-A Multiregional Study in Central and West Africa
Plasmodium falciparum parasites carrying deletions of histidine-rich protein 2 and 3 genes, pfhrp2 and pfhrp3, respectively, are likely to escape detection via HRP2-based rapid diagnostic tests (RDTs) and, consequently, treatment, posing a major risk to both the health of the infected individual and malaria control efforts. This study assessed the frequency of pfhrp2- and pfhrp3-deleted strains at four different study sites in Central Africa (number of samples analyzed: Gabon N = 534 and the Republic of Congo N = 917) and West Africa (number of samples analyzed: Nigeria N = 466 and Benin N = 120) using a highly sensitive multiplex qPCR. We found low prevalences for pfhrp2 (1%, 0%, 0.03% and 0) and pfhrp3 single deletions (0%, 0%, 0.03% and 0%) at all study sites (Gabon, the Republic of Congo, Nigeria and Benin, respectively). Double-deleted P. falciparum were only found in Nigeria in 1.6% of all internally controlled samples. The results of this pilot investigation do not point towards a high risk for false-negative RDT results due to pfhrp2/pfhrp3 deletions in Central and West African regions. However, as this scenario can change rapidly, continuous monitoring is essential to ensure that RDTs remain a suitable tool for the malaria diagnostic strategy.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:12 |
|---|---|
| Enthalten in: |
Pathogens (Basel, Switzerland) - 12(2023), 3 vom: 14. März |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Krueger, Tina [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Central Africa |
|---|
| Anmerkungen: |
Date Revised 31.03.2023 published: Electronic Citation Status PubMed-not-MEDLINE |
|---|
| doi: |
10.3390/pathogens12030455 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM354913409 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM354913409 | ||
| 003 | DE-627 | ||
| 005 | 20231226063233.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3390/pathogens12030455 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1182.xml |
| 035 | |a (DE-627)NLM354913409 | ||
| 035 | |a (NLM)36986377 | ||
| 035 | |a (PII)455 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Krueger, Tina |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Low Prevalence of Plasmodium falciparum Histidine-Rich Protein 2 and 3 Gene Deletions-A Multiregional Study in Central and West Africa |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 31.03.2023 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a Plasmodium falciparum parasites carrying deletions of histidine-rich protein 2 and 3 genes, pfhrp2 and pfhrp3, respectively, are likely to escape detection via HRP2-based rapid diagnostic tests (RDTs) and, consequently, treatment, posing a major risk to both the health of the infected individual and malaria control efforts. This study assessed the frequency of pfhrp2- and pfhrp3-deleted strains at four different study sites in Central Africa (number of samples analyzed: Gabon N = 534 and the Republic of Congo N = 917) and West Africa (number of samples analyzed: Nigeria N = 466 and Benin N = 120) using a highly sensitive multiplex qPCR. We found low prevalences for pfhrp2 (1%, 0%, 0.03% and 0) and pfhrp3 single deletions (0%, 0%, 0.03% and 0%) at all study sites (Gabon, the Republic of Congo, Nigeria and Benin, respectively). Double-deleted P. falciparum were only found in Nigeria in 1.6% of all internally controlled samples. The results of this pilot investigation do not point towards a high risk for false-negative RDT results due to pfhrp2/pfhrp3 deletions in Central and West African regions. However, as this scenario can change rapidly, continuous monitoring is essential to ensure that RDTs remain a suitable tool for the malaria diagnostic strategy | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Central Africa | |
| 650 | 4 | |a Plasmodium falciparum | |
| 650 | 4 | |a West Africa | |
| 650 | 4 | |a histidine-rich protein 2 and 3 | |
| 650 | 4 | |a pfhrp2/pfhrp3 deletions | |
| 650 | 4 | |a rapid diagnostic test | |
| 700 | 1 | |a Ikegbunam, Moses |e verfasserin |4 aut | |
| 700 | 1 | |a Lissom, Abel |e verfasserin |4 aut | |
| 700 | 1 | |a Sandri, Thaisa Lucas |e verfasserin |4 aut | |
| 700 | 1 | |a Ntabi, Jacques Dollon Mbama |e verfasserin |4 aut | |
| 700 | 1 | |a Djontu, Jean Claude |e verfasserin |4 aut | |
| 700 | 1 | |a Baina, Marcel Tapsou |e verfasserin |4 aut | |
| 700 | 1 | |a Lontchi, Roméo Aimé Laclong |e verfasserin |4 aut | |
| 700 | 1 | |a Maloum, Moustapha |e verfasserin |4 aut | |
| 700 | 1 | |a Ella, Givina Zang |e verfasserin |4 aut | |
| 700 | 1 | |a Agonhossou, Romuald |e verfasserin |4 aut | |
| 700 | 1 | |a Akoton, Romaric |e verfasserin |4 aut | |
| 700 | 1 | |a Djogbenou, Luc |e verfasserin |4 aut | |
| 700 | 1 | |a Borrmann, Steffen |e verfasserin |4 aut | |
| 700 | 1 | |a Held, Jana |e verfasserin |4 aut | |
| 700 | 1 | |a Ntoumi, Francine |e verfasserin |4 aut | |
| 700 | 1 | |a Adegnika, Ayola Akim |e verfasserin |4 aut | |
| 700 | 1 | |a Kremsner, Peter Gottfried |e verfasserin |4 aut | |
| 700 | 1 | |a Kreidenweiss, Andrea |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Pathogens (Basel, Switzerland) |d 2011 |g 12(2023), 3 vom: 14. März |w (DE-627)NLM22512761X |x 2076-0817 |7 nnns |
| 773 | 1 | 8 | |g volume:12 |g year:2023 |g number:3 |g day:14 |g month:03 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.3390/pathogens12030455 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 12 |j 2023 |e 3 |b 14 |c 03 | ||