Alterations in the Expression of IFN Lambda, IFN Gamma and Toll-like Receptors in Severe COVID-19 Patients
Contradictory results have been reported regarding interferon (IFN) lambda (λ1-3) and IFN gamma (γ) production in COVID-19 patients. To gain insight into the roles played by these IFNs in SARS-CoV-2 infection, IFNλ1-3 and IFNγ mRNA expression was evaluated in peripheral blood mononuclear cells (PBMCs) (n = 32) and in cells of paired bronchoalveolar lavages (BALs) (n = 12). Lower IFNλ1-3 values (p < 0.001 for IFNλ1 and 3 and p = 0.013 for IFNλ2) in the PBMCs of severely ill patients were found compared to healthy donors (n = 15). Reduced levels of IFNγ were also detected in patients' PBMCs (p < 0.01) and BALs (p = 0.041) compared to healthy donors. The presence of secondary bacterial infections was associated with decreased IFNλ amounts in PBMCs (p = 0.001, p = 0.015 and p = 0.003, respectively) but increased concentrations of IFNλ3 (p = 0.022) in BALs. Patients with alterations in C-reactive protein, lactate dehydrogenase and D-dimer levels had decreased IFNλ1 and 3 (p = 0.003 and p < 0.001) and increased IFNγ (p = 0.08) in PBMCs. Analyzing Toll-like receptors (TLRs) involved in IFN production, we found that TLR3 was highly expressed (p = 0.033) in patients with bacterial superinfections, while TLR7 and 8 (p = 0.029 and p = 0.049) were reduced in BALs of deceased patients. Overall, severe COVID-19 might be characterized by dysregulation in IFNγ, IFNλ and TLR3, 7 and 8 production.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2023 |
---|---|
Erschienen: |
2023 |
Enthalten in: |
Zur Gesamtaufnahme - volume:11 |
---|---|
Enthalten in: |
Microorganisms - 11(2023), 3 vom: 08. März |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Sorrentino, Leonardo [VerfasserIn] |
---|
Links: |
---|
Themen: |
---|
Anmerkungen: |
Date Revised 31.03.2023 published: Electronic Citation Status PubMed-not-MEDLINE |
---|
doi: |
10.3390/microorganisms11030689 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM354902261 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM354902261 | ||
003 | DE-627 | ||
005 | 20231226063219.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231226s2023 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.3390/microorganisms11030689 |2 doi | |
028 | 5 | 2 | |a pubmed24n1182.xml |
035 | |a (DE-627)NLM354902261 | ||
035 | |a (NLM)36985262 | ||
035 | |a (PII)689 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Sorrentino, Leonardo |e verfasserin |4 aut | |
245 | 1 | 0 | |a Alterations in the Expression of IFN Lambda, IFN Gamma and Toll-like Receptors in Severe COVID-19 Patients |
264 | 1 | |c 2023 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Revised 31.03.2023 | ||
500 | |a published: Electronic | ||
500 | |a Citation Status PubMed-not-MEDLINE | ||
520 | |a Contradictory results have been reported regarding interferon (IFN) lambda (λ1-3) and IFN gamma (γ) production in COVID-19 patients. To gain insight into the roles played by these IFNs in SARS-CoV-2 infection, IFNλ1-3 and IFNγ mRNA expression was evaluated in peripheral blood mononuclear cells (PBMCs) (n = 32) and in cells of paired bronchoalveolar lavages (BALs) (n = 12). Lower IFNλ1-3 values (p < 0.001 for IFNλ1 and 3 and p = 0.013 for IFNλ2) in the PBMCs of severely ill patients were found compared to healthy donors (n = 15). Reduced levels of IFNγ were also detected in patients' PBMCs (p < 0.01) and BALs (p = 0.041) compared to healthy donors. The presence of secondary bacterial infections was associated with decreased IFNλ amounts in PBMCs (p = 0.001, p = 0.015 and p = 0.003, respectively) but increased concentrations of IFNλ3 (p = 0.022) in BALs. Patients with alterations in C-reactive protein, lactate dehydrogenase and D-dimer levels had decreased IFNλ1 and 3 (p = 0.003 and p < 0.001) and increased IFNγ (p = 0.08) in PBMCs. Analyzing Toll-like receptors (TLRs) involved in IFN production, we found that TLR3 was highly expressed (p = 0.033) in patients with bacterial superinfections, while TLR7 and 8 (p = 0.029 and p = 0.049) were reduced in BALs of deceased patients. Overall, severe COVID-19 might be characterized by dysregulation in IFNγ, IFNλ and TLR3, 7 and 8 production | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a COVID-19 | |
650 | 4 | |a immunology | |
650 | 4 | |a interferon | |
700 | 1 | |a Fracella, Matteo |e verfasserin |4 aut | |
700 | 1 | |a Frasca, Federica |e verfasserin |4 aut | |
700 | 1 | |a D'Auria, Alessandra |e verfasserin |4 aut | |
700 | 1 | |a Santinelli, Letizia |e verfasserin |4 aut | |
700 | 1 | |a Maddaloni, Luca |e verfasserin |4 aut | |
700 | 1 | |a Bugani, Ginevra |e verfasserin |4 aut | |
700 | 1 | |a Bitossi, Camilla |e verfasserin |4 aut | |
700 | 1 | |a Gentile, Massimo |e verfasserin |4 aut | |
700 | 1 | |a Ceccarelli, Giancarlo |e verfasserin |4 aut | |
700 | 1 | |a Turriziani, Ombretta |e verfasserin |4 aut | |
700 | 1 | |a Mastroianni, Claudio Maria |e verfasserin |4 aut | |
700 | 1 | |a Antonelli, Guido |e verfasserin |4 aut | |
700 | 1 | |a d'Ettorre, Gabriella |e verfasserin |4 aut | |
700 | 1 | |a Pierangeli, Alessandra |e verfasserin |4 aut | |
700 | 1 | |a Scagnolari, Carolina |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Microorganisms |d 2013 |g 11(2023), 3 vom: 08. März |w (DE-627)NLM247050237 |x 2076-2607 |7 nnns |
773 | 1 | 8 | |g volume:11 |g year:2023 |g number:3 |g day:08 |g month:03 |
856 | 4 | 0 | |u http://dx.doi.org/10.3390/microorganisms11030689 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 11 |j 2023 |e 3 |b 08 |c 03 |