H2S-Enriched Flush out Does Not Increase Donor Organ Quality in a Porcine Kidney Perfusion Model
Kidney extraction time has a detrimental effect on post-transplantation outcome. This study aims to improve the flush-out and potentially decrease ischemic injury by the addition of hydrogen sulphide (H2S) to the flush medium. Porcine kidneys (n = 22) were extracted during organ recovery surgery. Pigs underwent brain death induction or a Sham operation, resulting in four groups: donation after brain death (DBD) control, DBD H2S, non-DBD control, and non-DBD H2S. Directly after the abdominal flush, kidneys were extracted and flushed with or without H2S and stored for 13 h via static cold storage (SCS) +/- H2S before reperfusion on normothermic machine perfusion. Pro-inflammatory cytokines IL-1b and IL-8 were significantly lower in H2S treated DBD kidneys during NMP (p = 0.03). The non-DBD kidneys show superiority in renal function (creatinine clearance and FENa) compared to the DBD control group (p = 0.03 and p = 0.004). No differences were seen in perfusion parameters, injury markers and histological appearance. We found an overall trend of better renal function in the non-DBD kidneys compared to the DBD kidneys. The addition of H2S during the flush out and SCS resulted in a reduction in pro-inflammatory cytokines without affecting renal function or injury markers.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:12 |
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| Enthalten in: |
Antioxidants (Basel, Switzerland) - 12(2023), 3 vom: 19. März |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Maassen, Hanno [VerfasserIn] |
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| Links: |
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| Themen: |
Brain death |
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| Anmerkungen: |
Date Revised 31.03.2023 published: Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.3390/antiox12030749 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Kidney extraction time has a detrimental effect on post-transplantation outcome. This study aims to improve the flush-out and potentially decrease ischemic injury by the addition of hydrogen sulphide (H2S) to the flush medium. Porcine kidneys (n = 22) were extracted during organ recovery surgery. Pigs underwent brain death induction or a Sham operation, resulting in four groups: donation after brain death (DBD) control, DBD H2S, non-DBD control, and non-DBD H2S. Directly after the abdominal flush, kidneys were extracted and flushed with or without H2S and stored for 13 h via static cold storage (SCS) +/- H2S before reperfusion on normothermic machine perfusion. Pro-inflammatory cytokines IL-1b and IL-8 were significantly lower in H2S treated DBD kidneys during NMP (p = 0.03). The non-DBD kidneys show superiority in renal function (creatinine clearance and FENa) compared to the DBD control group (p = 0.03 and p = 0.004). No differences were seen in perfusion parameters, injury markers and histological appearance. We found an overall trend of better renal function in the non-DBD kidneys compared to the DBD kidneys. The addition of H2S during the flush out and SCS resulted in a reduction in pro-inflammatory cytokines without affecting renal function or injury markers | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a H2S | |
| 650 | 4 | |a brain death | |
| 650 | 4 | |a cytokines | |
| 650 | 4 | |a ex vivo kidney perfusion | |
| 650 | 4 | |a hydrogen sulphide | |
| 650 | 4 | |a ischemia reperfusion injury | |
| 650 | 4 | |a kidney graft preservation | |
| 650 | 4 | |a transplantation | |
| 700 | 1 | |a Venema, Leonie H |e verfasserin |4 aut | |
| 700 | 1 | |a Weiss, Marc G |e verfasserin |4 aut | |
| 700 | 1 | |a Huijink, Tobias M |e verfasserin |4 aut | |
| 700 | 1 | |a Hofker, H Sijbrand |e verfasserin |4 aut | |
| 700 | 1 | |a Keller, Anna K |e verfasserin |4 aut | |
| 700 | 1 | |a Mollnes, Tom E |e verfasserin |4 aut | |
| 700 | 1 | |a Eijken, Marco |e verfasserin |4 aut | |
| 700 | 1 | |a Pischke, Søren E |e verfasserin |4 aut | |
| 700 | 1 | |a Jespersen, Bente |e verfasserin |4 aut | |
| 700 | 1 | |a van Goor, Harry |e verfasserin |4 aut | |
| 700 | 1 | |a Leuvenink, Henri G D |e verfasserin |4 aut | |
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