Detection of circulating cell-free HPV DNA of 13 HPV types for patients with cervical cancer as potential biomarker to monitor therapy response and to detect relapse
© 2023. The Author(s)..
BACKGROUND: HPV-related cervical cancer (CC) is the fourth most frequent cancer in women worldwide. Cell-free tumour DNA is a potent biomarker to detect treatment response, residual disease, and relapse. We investigated the potential use of cell-free circulating HPV-DNA (cfHPV-DNA) in plasma of patients with CC.
METHODS: cfHPV-DNA levels were measured using a highly sensitive next-generation sequencing-based approach targeting a panel of 13 high-risk HPV types.
RESULTS: Sequencing was performed in 69 blood samples collected from 35 patients, of which 26 were treatment-naive when the first liquid biopsy sample was retrieved. cfHPV-DNA was successfully detected in 22/26 (85%) cases. A significant correlation between tumour burden and cfHPV-DNA levels was observed: cfHPV-DNA was detectable in all treatment-naive patients with advanced-stage disease (17/17, FIGO IB3-IVB) and in 5/9 patients with early-stage disease (FIGO IA-IB2). Sequential samples revealed a decrease of cfHPV-DNA levels in 7 patients corresponding treatment response and an increase in a patient with relapse.
CONCLUSIONS: In this proof-of-concept study we demonstrated the potential of cfHPV-DNA as a biomarker for therapy monitoring in patients with primary and recurrent CC. Our findings facilitate the development of a sensitive and precise, non-invasive, inexpensive, and easily accessible tool in CC diagnosis, therapy monitoring and follow-up.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2023 |
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Erschienen: |
2023 |
Enthalten in: |
Zur Gesamtaufnahme - volume:128 |
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Enthalten in: |
British journal of cancer - 128(2023), 11 vom: 27. Juni, Seite 2097-2103 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Mittelstadt, Suzana [VerfasserIn] |
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Links: |
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Themen: |
Biomarkers |
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Anmerkungen: |
Date Completed 25.05.2023 Date Revised 29.05.2023 published: Print-Electronic Citation Status MEDLINE |
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doi: |
10.1038/s41416-023-02233-x |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM354784552 |
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520 | |a BACKGROUND: HPV-related cervical cancer (CC) is the fourth most frequent cancer in women worldwide. Cell-free tumour DNA is a potent biomarker to detect treatment response, residual disease, and relapse. We investigated the potential use of cell-free circulating HPV-DNA (cfHPV-DNA) in plasma of patients with CC | ||
520 | |a METHODS: cfHPV-DNA levels were measured using a highly sensitive next-generation sequencing-based approach targeting a panel of 13 high-risk HPV types | ||
520 | |a RESULTS: Sequencing was performed in 69 blood samples collected from 35 patients, of which 26 were treatment-naive when the first liquid biopsy sample was retrieved. cfHPV-DNA was successfully detected in 22/26 (85%) cases. A significant correlation between tumour burden and cfHPV-DNA levels was observed: cfHPV-DNA was detectable in all treatment-naive patients with advanced-stage disease (17/17, FIGO IB3-IVB) and in 5/9 patients with early-stage disease (FIGO IA-IB2). Sequential samples revealed a decrease of cfHPV-DNA levels in 7 patients corresponding treatment response and an increase in a patient with relapse | ||
520 | |a CONCLUSIONS: In this proof-of-concept study we demonstrated the potential of cfHPV-DNA as a biomarker for therapy monitoring in patients with primary and recurrent CC. Our findings facilitate the development of a sensitive and precise, non-invasive, inexpensive, and easily accessible tool in CC diagnosis, therapy monitoring and follow-up | ||
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700 | 1 | |a Gschwind, Axel |e verfasserin |4 aut | |
700 | 1 | |a Koch, André |e verfasserin |4 aut | |
700 | 1 | |a Wörz, Sarah |e verfasserin |4 aut | |
700 | 1 | |a Oberlechner, Ernst |e verfasserin |4 aut | |
700 | 1 | |a Engler, Tobias |e verfasserin |4 aut | |
700 | 1 | |a Bonzheim, Irina |e verfasserin |4 aut | |
700 | 1 | |a Staebler, Annette |e verfasserin |4 aut | |
700 | 1 | |a Weidner, Nicola |e verfasserin |4 aut | |
700 | 1 | |a Stubenrauch, Frank |e verfasserin |4 aut | |
700 | 1 | |a Iftner, Thomas |e verfasserin |4 aut | |
700 | 1 | |a Riess, Olaf |e verfasserin |4 aut | |
700 | 1 | |a Schroeder, Christopher |e verfasserin |4 aut | |
700 | 1 | |a Kommoss, Stefan |e verfasserin |4 aut | |
700 | 1 | |a Ossowski, Stephan |e verfasserin |4 aut | |
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