Optimal low-intensity pulsed ultrasound stimulation for promoting anti-inflammatory effects in macrophages
© 2023 Author(s)..
In this paper, we stimulated M1-like macrophages (obtained from U937 cells) with low-intensity pulsed ultrasound (LIPUS) to lower pro-inflammatory cytokine production. A systematic screening of different frequencies, intensities, duty cycles, and exposure times was performed. The optimal stimulation conditions leading to a marked decrease in the release of inflammatory cytokines were determined to be 38 kHz, 250 mW/cm2, 20%, and 90 min, respectively. Using these parameters, we verified that up to 72 h LIPUS did not affect cell viability, resulting in an increase in metabolic activity and in a reduction of reactive oxygen species (ROS) production. Moreover, we found that two mechanosensitive ion channels (PIEZO1 and TRPV1) were involved in the LIPUS-mediated cytokine release modulation. We also assessed the role of the nuclear factor κB (NF-κB) signaling pathway and observed an enhancement of actin polymerization. Finally, transcriptomic data suggested that the bioeffects of LIPUS treatment occur through the modulation of p38 MAPK signaling pathway.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:7 |
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| Enthalten in: |
APL bioengineering - 7(2023), 1 vom: 22. März, Seite 016114 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Iacoponi, Francesco [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Revised 28.03.2023 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1063/5.0137881 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM354735144 |
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| 520 | |a © 2023 Author(s). | ||
| 520 | |a In this paper, we stimulated M1-like macrophages (obtained from U937 cells) with low-intensity pulsed ultrasound (LIPUS) to lower pro-inflammatory cytokine production. A systematic screening of different frequencies, intensities, duty cycles, and exposure times was performed. The optimal stimulation conditions leading to a marked decrease in the release of inflammatory cytokines were determined to be 38 kHz, 250 mW/cm2, 20%, and 90 min, respectively. Using these parameters, we verified that up to 72 h LIPUS did not affect cell viability, resulting in an increase in metabolic activity and in a reduction of reactive oxygen species (ROS) production. Moreover, we found that two mechanosensitive ion channels (PIEZO1 and TRPV1) were involved in the LIPUS-mediated cytokine release modulation. We also assessed the role of the nuclear factor κB (NF-κB) signaling pathway and observed an enhancement of actin polymerization. Finally, transcriptomic data suggested that the bioeffects of LIPUS treatment occur through the modulation of p38 MAPK signaling pathway | ||
| 650 | 4 | |a Journal Article | |
| 700 | 1 | |a Cafarelli, Andrea |e verfasserin |4 aut | |
| 700 | 1 | |a Fontana, Francesco |e verfasserin |4 aut | |
| 700 | 1 | |a Pratellesi, Tiziano |e verfasserin |4 aut | |
| 700 | 1 | |a Dumont, Erik |e verfasserin |4 aut | |
| 700 | 1 | |a Barravecchia, Ivana |e verfasserin |4 aut | |
| 700 | 1 | |a Angeloni, Debora |e verfasserin |4 aut | |
| 700 | 1 | |a Ricotti, Leonardo |e verfasserin |4 aut | |
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