Investigating the renoprotective effect of C21 in male mice with sepsis via modulation of p-AKT/PI3K expression
©2023 JOURNAL of MEDICINE and LIFE..
This study aimed to investigate if C21 could prevent acute renal injury induced by sepsis by regulating the expression of p-AKT/PI3K. Five equal groups of 25 adult male Swiss-albino mice were randomly divided (n=5): sham (laparotomy without CLP), CLP, vehicle (equivalent amount of DMSO one hour before CLP), and C21 (0.03 mg/kg, one hour before CLP). ELISA was used to measure serum inflammatory mediators, and the expression of PI3K and P-AKT was determined using PCR and immunohistochemistry (IHC), respectively. TNF, TNF receptor, F8-isoprostane, urea, creatinine, and IL-6 blood levels were considerably lower in the CLP group (p<0.05) compared to the sham group, whereas the C21 treated group had significantly (p<0.05) greater levels of these inflammatory mediators. The IHC analysis revealed that P-AKT expression was significantly lower (p<0.05) in the CLP group compared to the sham group, while the C21 pretreatment group had significantly higher levels of P-AKT expression compared to the CLP group (p<0.05). The PI3K expression in the CLP group was significantly lower than in the sham group (p<0.05), according to PCR results, whereas the PI3K expression in the C21 pretreatment group was significantly greater than in the CLP group (p<0.05). This study showed that C21 might reduce levels of pro-inflammatory cytokines, including TNF-, IL-6, and TNF receptor, by modulating the PI3K/AKT signaling pathways, which can, in turn, reduce renal dysfunction during CLP-induced sepsis in male mice.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:16 |
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| Enthalten in: |
Journal of medicine and life - 16(2023), 2 vom: 23. Feb., Seite 203-209 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Jabber, Huda [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 21.03.2023 Date Revised 21.03.2023 published: Print Citation Status MEDLINE |
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| doi: |
10.25122/jml-2022-0299 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM354428721 |
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| 245 | 1 | 0 | |a Investigating the renoprotective effect of C21 in male mice with sepsis via modulation of p-AKT/PI3K expression |
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| 520 | |a This study aimed to investigate if C21 could prevent acute renal injury induced by sepsis by regulating the expression of p-AKT/PI3K. Five equal groups of 25 adult male Swiss-albino mice were randomly divided (n=5): sham (laparotomy without CLP), CLP, vehicle (equivalent amount of DMSO one hour before CLP), and C21 (0.03 mg/kg, one hour before CLP). ELISA was used to measure serum inflammatory mediators, and the expression of PI3K and P-AKT was determined using PCR and immunohistochemistry (IHC), respectively. TNF, TNF receptor, F8-isoprostane, urea, creatinine, and IL-6 blood levels were considerably lower in the CLP group (p<0.05) compared to the sham group, whereas the C21 treated group had significantly (p<0.05) greater levels of these inflammatory mediators. The IHC analysis revealed that P-AKT expression was significantly lower (p<0.05) in the CLP group compared to the sham group, while the C21 pretreatment group had significantly higher levels of P-AKT expression compared to the CLP group (p<0.05). The PI3K expression in the CLP group was significantly lower than in the sham group (p<0.05), according to PCR results, whereas the PI3K expression in the C21 pretreatment group was significantly greater than in the CLP group (p<0.05). This study showed that C21 might reduce levels of pro-inflammatory cytokines, including TNF-, IL-6, and TNF receptor, by modulating the PI3K/AKT signaling pathways, which can, in turn, reduce renal dysfunction during CLP-induced sepsis in male mice | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a ACR – Urinary Albumin and Creatinine | |
| 650 | 4 | |a AKT – Acute Kidney Injury | |
| 650 | 4 | |a Acute kidney injury | |
| 650 | 4 | |a BUN – Blood Urea Nitrogen | |
| 650 | 4 | |a C21 | |
| 650 | 4 | |a CDC – Centers for Disease Control and Prevention | |
| 650 | 4 | |a CLP – Cecal Ligation Procedure | |
| 650 | 4 | |a IHC – Immunohistochemistry | |
| 650 | 4 | |a P-AKT – Phosphorylated Protein kinase B | |
| 650 | 4 | |a P-AKT/PI3K | |
| 650 | 4 | |a PCR – Polymerase Chain Reaction | |
| 650 | 4 | |a PI3K – Phosphatidylinositol-3-kinase | |
| 650 | 4 | |a RAS – Renin-Angiotensin System | |
| 650 | 4 | |a SCR – Serum Creatinine | |
| 650 | 4 | |a UF – Urine Flow | |
| 650 | 4 | |a UNaV – Urinary Na Volume | |
| 650 | 4 | |a mice | |
| 650 | 4 | |a sepsis | |
| 650 | 7 | |a Phosphatidylinositol 3-Kinases |2 NLM | |
| 650 | 7 | |a EC 2.7.1.- |2 NLM | |
| 650 | 7 | |a Proto-Oncogene Proteins c-akt |2 NLM | |
| 650 | 7 | |a EC 2.7.11.1 |2 NLM | |
| 650 | 7 | |a Interleukin-6 |2 NLM | |
| 650 | 7 | |a compound 21 |2 NLM | |
| 650 | 7 | |a RC2V4W0EYC |2 NLM | |
| 650 | 7 | |a Inflammation Mediators |2 NLM | |
| 700 | 1 | |a Mohammed, Bassim |e verfasserin |4 aut | |
| 700 | 1 | |a Hadi, Najah Rayish |e verfasserin |4 aut | |
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