Assays Based on Pseudotyped Viruses
© 2023. The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd..
Pseudotyped viruses are more and more widely used in virus research and the evaluation of antiviral products because of their high safety, simple operation, high accessibility, ease in achieving standardization, and high throughput. The development of measures based on pseudotyped virus is closely related to the characteristics of viruses, and it is also necessary to follow the principles of assay development. Only in the process of method development, where the key parameters that affect the results are systematically optimized and the preliminary established method is fully validated, can the accuracy, reliability, and repeatability of the test results be ensured. Only the method established on this basis can be transferred to different laboratories and make the results of different laboratories comparable. This paper summarizes the specific aspects and general principles in the development of assays based on pseudotyped virus, which is of reference value for the development of similar methods.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:1407 |
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| Enthalten in: |
Advances in experimental medicine and biology - 1407(2023) vom: 15., Seite 29-44 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Nie, Jianhui [VerfasserIn] |
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| Links: |
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| Themen: |
Animal model |
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| Anmerkungen: |
Date Completed 20.03.2023 Date Revised 20.03.2023 published: Print Citation Status MEDLINE |
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| doi: |
10.1007/978-981-99-0113-5_2 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Pseudotyped viruses are more and more widely used in virus research and the evaluation of antiviral products because of their high safety, simple operation, high accessibility, ease in achieving standardization, and high throughput. The development of measures based on pseudotyped virus is closely related to the characteristics of viruses, and it is also necessary to follow the principles of assay development. Only in the process of method development, where the key parameters that affect the results are systematically optimized and the preliminary established method is fully validated, can the accuracy, reliability, and repeatability of the test results be ensured. Only the method established on this basis can be transferred to different laboratories and make the results of different laboratories comparable. This paper summarizes the specific aspects and general principles in the development of assays based on pseudotyped virus, which is of reference value for the development of similar methods | ||
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