Photo-crosslinked gelatin methacryloyl hydrogel strengthened with calcium phosphate-based nanoparticles for early healing of rabbit calvarial defects
Copyright © 2023. Korean Academy of Periodontology..
PURPOSE: The aim of this study was to investigate the efficacy of photo-crosslinked gelatin methacryloyl (GelMa) hydrogel containing calcium phosphate nanoparticles (CNp) when applying different fabrication methods for bone regeneration.
METHODS: Four circular defects were created in the calvaria of 10 rabbits. Each defect was randomly allocated to the following study groups: 1) the sham control group, 2) the GelMa group (defect filled with crosslinked GelMa hydrogel), 3) the CNp-GelMa group (GelMa hydrogel crosslinked with nanoparticles), and 4) the CNp+GelMa group (crosslinked GelMa loaded with nanoparticles). At 2, 4, and 8 weeks, samples were harvested, and histological and micro-computed tomography analyses were performed.
RESULTS: Histomorphometric analysis showed that the CNp-GelMa and CNp+GelMa groups at 2 weeks had significantly greater total augmented areas than the control group (P<0.05). The greatest new bone area was observed in the CNp-GelMa group, but without statistical significance (P>0.05). Crosslinked GelMa hydrogel with nanoparticles exhibited good biocompatibility with a minimal inflammatory reaction.
CONCLUSIONS: There was no difference in the efficacy of bone regeneration according to the synthesized method of photo-crosslinked GelMa hydrogel with nanoparticles. However, these materials could remain within a bone defect up to 2 weeks and showed good biocompatibility with little inflammatory response. Further improvement in mechanical properties and resistance to enzymatic degradation would be needed for the clinical application.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:53 |
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| Enthalten in: |
Journal of periodontal & implant science - 53(2023), 5 vom: 14. Okt., Seite 321-335 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Lee, Da-Na [VerfasserIn] |
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| Links: |
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| Themen: |
Animals |
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| Anmerkungen: |
Date Revised 08.11.2023 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.5051/jpis.2203220161 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM354245538 |
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| 100 | 1 | |a Lee, Da-Na |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Photo-crosslinked gelatin methacryloyl hydrogel strengthened with calcium phosphate-based nanoparticles for early healing of rabbit calvarial defects |
| 264 | 1 | |c 2023 | |
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| 500 | |a Date Revised 08.11.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a Copyright © 2023. Korean Academy of Periodontology. | ||
| 520 | |a PURPOSE: The aim of this study was to investigate the efficacy of photo-crosslinked gelatin methacryloyl (GelMa) hydrogel containing calcium phosphate nanoparticles (CNp) when applying different fabrication methods for bone regeneration | ||
| 520 | |a METHODS: Four circular defects were created in the calvaria of 10 rabbits. Each defect was randomly allocated to the following study groups: 1) the sham control group, 2) the GelMa group (defect filled with crosslinked GelMa hydrogel), 3) the CNp-GelMa group (GelMa hydrogel crosslinked with nanoparticles), and 4) the CNp+GelMa group (crosslinked GelMa loaded with nanoparticles). At 2, 4, and 8 weeks, samples were harvested, and histological and micro-computed tomography analyses were performed | ||
| 520 | |a RESULTS: Histomorphometric analysis showed that the CNp-GelMa and CNp+GelMa groups at 2 weeks had significantly greater total augmented areas than the control group (P<0.05). The greatest new bone area was observed in the CNp-GelMa group, but without statistical significance (P>0.05). Crosslinked GelMa hydrogel with nanoparticles exhibited good biocompatibility with a minimal inflammatory reaction | ||
| 520 | |a CONCLUSIONS: There was no difference in the efficacy of bone regeneration according to the synthesized method of photo-crosslinked GelMa hydrogel with nanoparticles. However, these materials could remain within a bone defect up to 2 weeks and showed good biocompatibility with little inflammatory response. Further improvement in mechanical properties and resistance to enzymatic degradation would be needed for the clinical application | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Animals | |
| 650 | 4 | |a Biomaterials | |
| 650 | 4 | |a Bone regeneration | |
| 650 | 4 | |a Gelatin methacryloyl | |
| 650 | 4 | |a Nanoparticles | |
| 700 | 1 | |a Park, Jin-Young |e verfasserin |4 aut | |
| 700 | 1 | |a Seo, Young-Wook |e verfasserin |4 aut | |
| 700 | 1 | |a Jin, Xiang |e verfasserin |4 aut | |
| 700 | 1 | |a Hong, Jongmin |e verfasserin |4 aut | |
| 700 | 1 | |a Bhattacharyya, Amitava |e verfasserin |4 aut | |
| 700 | 1 | |a Noh, Insup |e verfasserin |4 aut | |
| 700 | 1 | |a Choi, Seong-Ho |e verfasserin |4 aut | |
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