B cell receptor repertoire analysis from autopsy samples of COVID-19 patients
Copyright © 2023 Iwabuchi, Tsukahara, Okayama, Kitabatake, Motobayashi, Shichino, Imafuku, Yamaji, Miyamoto, Tamura, Ueha, Ito, Murata, Kondo, Ikeo, Suzuki, Matsushima, Kohara, Torigoe, Yamaue and Hashimoto..
Neutralizing antibodies against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are being developed world over. We investigated the possibility of producing artificial antibodies from the formalin fixation and paraffin-embedding (FFPE) lung lobes of a patient who died by coronavirus disease 2019 (COVID-19). The B-cell receptors repertoire in the lung tissue where SARS-CoV-2 was detected were considered to have highly sensitive virus-neutralizing activity, and artificial antibodies were produced by combining the most frequently detected heavy and light chains. Some neutralizing effects against the SARS-CoV-2 were observed, and mixing two different artificial antibodies had a higher tendency to suppress the virus. The neutralizing effects were similar to the immunoglobulin G obtained from healthy donors who had received a COVID-19 mRNA vaccine. Therefore, the use of FFPE lung tissue, which preserves the condition of direct virus sensitization, to generate artificial antibodies may be useful against future unknown infectious diseases.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:14 |
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| Enthalten in: |
Frontiers in immunology - 14(2023) vom: 05., Seite 1034978 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Iwabuchi, Sadahiro [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 14.03.2023 Date Revised 27.03.2023 published: Electronic-eCollection Citation Status MEDLINE |
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| doi: |
10.3389/fimmu.2023.1034978 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM354172913 |
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| 245 | 1 | 0 | |a B cell receptor repertoire analysis from autopsy samples of COVID-19 patients |
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| 520 | |a Copyright © 2023 Iwabuchi, Tsukahara, Okayama, Kitabatake, Motobayashi, Shichino, Imafuku, Yamaji, Miyamoto, Tamura, Ueha, Ito, Murata, Kondo, Ikeo, Suzuki, Matsushima, Kohara, Torigoe, Yamaue and Hashimoto. | ||
| 520 | |a Neutralizing antibodies against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are being developed world over. We investigated the possibility of producing artificial antibodies from the formalin fixation and paraffin-embedding (FFPE) lung lobes of a patient who died by coronavirus disease 2019 (COVID-19). The B-cell receptors repertoire in the lung tissue where SARS-CoV-2 was detected were considered to have highly sensitive virus-neutralizing activity, and artificial antibodies were produced by combining the most frequently detected heavy and light chains. Some neutralizing effects against the SARS-CoV-2 were observed, and mixing two different artificial antibodies had a higher tendency to suppress the virus. The neutralizing effects were similar to the immunoglobulin G obtained from healthy donors who had received a COVID-19 mRNA vaccine. Therefore, the use of FFPE lung tissue, which preserves the condition of direct virus sensitization, to generate artificial antibodies may be useful against future unknown infectious diseases | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a B cell receptors | |
| 650 | 4 | |a COVID-19 | |
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| 650 | 4 | |a vaccine intake | |
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| 650 | 7 | |a Antibodies, Neutralizing |2 NLM | |
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| 650 | 7 | |a Receptors, Antigen, B-Cell |2 NLM | |
| 700 | 1 | |a Tsukahara, Tomohide |e verfasserin |4 aut | |
| 700 | 1 | |a Okayama, Toshitugu |e verfasserin |4 aut | |
| 700 | 1 | |a Kitabatake, Masahiro |e verfasserin |4 aut | |
| 700 | 1 | |a Motobayashi, Hideki |e verfasserin |4 aut | |
| 700 | 1 | |a Shichino, Shigeyuki |e verfasserin |4 aut | |
| 700 | 1 | |a Imafuku, Tadashi |e verfasserin |4 aut | |
| 700 | 1 | |a Yamaji, Kenzaburo |e verfasserin |4 aut | |
| 700 | 1 | |a Miyamoto, Kyohei |e verfasserin |4 aut | |
| 700 | 1 | |a Tamura, Shinobu |e verfasserin |4 aut | |
| 700 | 1 | |a Ueha, Satoshi |e verfasserin |4 aut | |
| 700 | 1 | |a Ito, Toshihiro |e verfasserin |4 aut | |
| 700 | 1 | |a Murata, Shin-Ichi |e verfasserin |4 aut | |
| 700 | 1 | |a Kondo, Toshikazu |e verfasserin |4 aut | |
| 700 | 1 | |a Ikeo, Kazuho |e verfasserin |4 aut | |
| 700 | 1 | |a Suzuki, Yutaka |e verfasserin |4 aut | |
| 700 | 1 | |a Matsushima, Kouji |e verfasserin |4 aut | |
| 700 | 1 | |a Kohara, Michinori |e verfasserin |4 aut | |
| 700 | 1 | |a Torigoe, Toshihiko |e verfasserin |4 aut | |
| 700 | 1 | |a Yamaue, Hiroki |e verfasserin |4 aut | |
| 700 | 1 | |a Hashimoto, Shinichi |e verfasserin |4 aut | |
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