NAT10 mediated mRNA acetylation modification patterns associated with colon cancer progression and microsatellite status
N4-acetylcytidine (ac4C) is one type of RNA modification found in eukaryotes. RNA acetylation modifications are gradually expanding in oncology. However, the role of RNA acetylation modifications in colorectal cancer and its association with colorectal cancer microsatellite status remain unclear. Using public databases and in vitro experiments, we verified the expression and biological function of NAT10, as the key RNA acetylation modification enzyme, in colorectal cancer. The results showed that NAT10 was highly expressed in colorectal cancer, and significantly promoted colorectal cancer cell proliferation. NAT10 was also involved in several aspects of cell homoeostasis such as ion transport, calcium-dependent phospholipid binding, and RNA stability. NAT10 expression positively correlated with immune infiltration in colorectal cancer. We further constructed a risk regression model for mRNA acetylation in colorectal cancer using acetylation-related differential genes. We found that tumour immune infiltration, microsatellite instability (MSI) proportion, tumour immune mutation burden, and patient response to immunotherapy were positively correlated with risk scores. For the first time, our study showed that the level of mRNA acetylation modification level is elevated in colorectal cancer and positively correlates with immune infiltration and microsatellite status of patients. Based on our findings, NAT10 may be a new target for colorectal cancer treatment.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:18 |
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| Enthalten in: |
Epigenetics - 18(2023), 1 vom: 13. Dez., Seite 2188667 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhang, Hailin [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 14.03.2023 Date Revised 22.03.2023 published: Print Citation Status MEDLINE |
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| doi: |
10.1080/15592294.2023.2188667 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM354136577 |
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| 245 | 1 | 0 | |a NAT10 mediated mRNA acetylation modification patterns associated with colon cancer progression and microsatellite status |
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| 520 | |a N4-acetylcytidine (ac4C) is one type of RNA modification found in eukaryotes. RNA acetylation modifications are gradually expanding in oncology. However, the role of RNA acetylation modifications in colorectal cancer and its association with colorectal cancer microsatellite status remain unclear. Using public databases and in vitro experiments, we verified the expression and biological function of NAT10, as the key RNA acetylation modification enzyme, in colorectal cancer. The results showed that NAT10 was highly expressed in colorectal cancer, and significantly promoted colorectal cancer cell proliferation. NAT10 was also involved in several aspects of cell homoeostasis such as ion transport, calcium-dependent phospholipid binding, and RNA stability. NAT10 expression positively correlated with immune infiltration in colorectal cancer. We further constructed a risk regression model for mRNA acetylation in colorectal cancer using acetylation-related differential genes. We found that tumour immune infiltration, microsatellite instability (MSI) proportion, tumour immune mutation burden, and patient response to immunotherapy were positively correlated with risk scores. For the first time, our study showed that the level of mRNA acetylation modification level is elevated in colorectal cancer and positively correlates with immune infiltration and microsatellite status of patients. Based on our findings, NAT10 may be a new target for colorectal cancer treatment | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a NAT10 | |
| 650 | 4 | |a colorectal cancer | |
| 650 | 4 | |a mRNA acetylation | |
| 650 | 4 | |a microsatellite status | |
| 650 | 4 | |a tumor immune infiltration | |
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| 650 | 7 | |a N-Terminal Acetyltransferases |2 NLM | |
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| 650 | 7 | |a NAT10 protein, human |2 NLM | |
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| 700 | 1 | |a Shan, Wenqing |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Zhenwei |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Yangyang |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Meng |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, Liping |e verfasserin |4 aut | |
| 700 | 1 | |a Zeng, Lingxiu |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Qiu |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Jing |e verfasserin |4 aut | |
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