Water-soluble protein from walleye pollock (Gadus chalcogrammus) suppresses lipopolysaccharide-induced inflammation by attenuating TLR4-MyD88 expression in macrophages
© 2023 The Authors..
Water-soluble protein (WSP) from fish meat is abundant in the waste effluent generated via the surimi manufacturing process. This study investigated the anti-inflammatory effects and mechanisms of fish WSP using primary macrophages (MΦ) and animal ingestion. MΦ were treated with digested-WSP (d-WSP, 500 µg/mL) with or without lipopolysaccharide (LPS) stimulation. For the ingestion study, male ICR mice (5 weeks old) were fed 4% WSP for 14 days following LPS administration (4 mg/kg body weight). d-WSP decreased the expression of Tlr4, an LPS receptor. Additionally, d-WSP significantly suppressed the secretion of inflammatory cytokines, phagocytic ability, and Myd88 and Il1b expressions of LPS-stimulated macrophages. Furthermore, the ingestion of 4% WSP attenuated not only LPS-induced IL-1β secretion in the blood but also Myd88 and Il1b expressions in the liver. Thus, fish WSP decreases the expressions of the genes involved in the TLR4-MyD88 pathway in MΦ and the liver, thereby suppressing inflammation.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:6 |
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| Enthalten in: |
Food chemistry. Molecular sciences - 6(2023) vom: 30. Juli, Seite 100165 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ono, Masataka [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Revised 10.03.2023 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1016/j.fochms.2023.100165 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM353971812 |
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| 024 | 7 | |a 10.1016/j.fochms.2023.100165 |2 doi | |
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| 041 | |a eng | ||
| 100 | 1 | |a Ono, Masataka |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Water-soluble protein from walleye pollock (Gadus chalcogrammus) suppresses lipopolysaccharide-induced inflammation by attenuating TLR4-MyD88 expression in macrophages |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 10.03.2023 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a © 2023 The Authors. | ||
| 520 | |a Water-soluble protein (WSP) from fish meat is abundant in the waste effluent generated via the surimi manufacturing process. This study investigated the anti-inflammatory effects and mechanisms of fish WSP using primary macrophages (MΦ) and animal ingestion. MΦ were treated with digested-WSP (d-WSP, 500 µg/mL) with or without lipopolysaccharide (LPS) stimulation. For the ingestion study, male ICR mice (5 weeks old) were fed 4% WSP for 14 days following LPS administration (4 mg/kg body weight). d-WSP decreased the expression of Tlr4, an LPS receptor. Additionally, d-WSP significantly suppressed the secretion of inflammatory cytokines, phagocytic ability, and Myd88 and Il1b expressions of LPS-stimulated macrophages. Furthermore, the ingestion of 4% WSP attenuated not only LPS-induced IL-1β secretion in the blood but also Myd88 and Il1b expressions in the liver. Thus, fish WSP decreases the expressions of the genes involved in the TLR4-MyD88 pathway in MΦ and the liver, thereby suppressing inflammation | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a 2Me, 2-mercaptoethanol | |
| 650 | 4 | |a Anti-inflammation | |
| 650 | 4 | |a Aq, aqua | |
| 650 | 4 | |a E. coli, Escherichia coli | |
| 650 | 4 | |a ELISA, Enzyme-linked immunosorbent assay | |
| 650 | 4 | |a FBS, Fetal bovine serum | |
| 650 | 4 | |a Fish water-soluble protein | |
| 650 | 4 | |a IL-1β, Interleukin 1 beta | |
| 650 | 4 | |a IL-6, Interleukin 6 | |
| 650 | 4 | |a LBP, Lipopolysaccharide-binding protein | |
| 650 | 4 | |a LPS | |
| 650 | 4 | |a LPS, Lipopolysaccharide | |
| 650 | 4 | |a Macrophage | |
| 650 | 4 | |a MyD88 | |
| 650 | 4 | |a MyD88, Myeloid differentiation primary response 88 | |
| 650 | 4 | |a NCDs, Noncommunicable diseases | |
| 650 | 4 | |a NF-κB, Nuclear factor-kappa B | |
| 650 | 4 | |a NLRP3, NACHT, LRR, and PYD domain-containing protein 3 | |
| 650 | 4 | |a PBS, Phosphate-buffered saline | |
| 650 | 4 | |a PCR, Polymerase chain reaction | |
| 650 | 4 | |a SDS, Sodium dodecyl sulfate | |
| 650 | 4 | |a TICAM-1, Toll-like receptor adaptor molecule 1 | |
| 650 | 4 | |a TLR, Toll-like receptor | |
| 650 | 4 | |a TLR4 | |
| 650 | 4 | |a TLR4, Toll-like receptor 4 | |
| 650 | 4 | |a TNF-α, Tumor necrosis factor-alpha | |
| 650 | 4 | |a TNFR, Tumor necrosis factor receptor | |
| 650 | 4 | |a TRIF, TIR-domain–containing adapter-inducing interferon-beta | |
| 650 | 4 | |a WSP, Water-soluble protein | |
| 650 | 4 | |a d-WSP, digested water-soluble protein | |
| 700 | 1 | |a Watari, Satomi |e verfasserin |4 aut | |
| 700 | 1 | |a Nishizawa-Higashi, Mizuho |e verfasserin |4 aut | |
| 700 | 1 | |a Konishi, Tatsuya |e verfasserin |4 aut | |
| 700 | 1 | |a Takahashi, Yoshinori |e verfasserin |4 aut | |
| 700 | 1 | |a Saeki, Hiroki |e verfasserin |4 aut | |
| 700 | 1 | |a Joe, Ga-Hyun |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Food chemistry. Molecular sciences |d 2020 |g 6(2023) vom: 30. Juli, Seite 100165 |w (DE-627)NLM339451475 |x 2666-5662 |7 nnns |
| 773 | 1 | 8 | |g volume:6 |g year:2023 |g day:30 |g month:07 |g pages:100165 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.fochms.2023.100165 |3 Volltext |
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