Noncoding Regulatory RNAs : Isolation and Analysis of Neuronal Circular RNAs and MicroRNAs
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature..
In addition to expressing a large number of protein-coding transcripts, including alternatively spliced isoforms of the same mRNAs, neurons express a large number of noncoding RNAs. These include microRNAs (miRNAs), circular RNAs (circRNAs), and other regulatory RNAs. The isolation and quantitative analyses of diverse types of RNAs in neurons are critical to understand not only the posttranscriptional mechanisms regulating mRNA levels and their translation but also the potential of several RNAs expressed in the same neurons to regulate these processes by generating networks of competing endogenous RNAs (ceRNAs). This chapter will describe methods for the isolation and analyses of circRNA and miRNA levels from the same brain tissue sample.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2636 |
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| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2636(2023) vom: 07., Seite 71-83 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Dell'Orco, Michela [VerfasserIn] |
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| Links: |
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| Themen: |
Brain tissues |
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| Anmerkungen: |
Date Completed 09.03.2023 Date Revised 26.04.2023 published: Print Citation Status MEDLINE |
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| doi: |
10.1007/978-1-0716-3012-9_5 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a In addition to expressing a large number of protein-coding transcripts, including alternatively spliced isoforms of the same mRNAs, neurons express a large number of noncoding RNAs. These include microRNAs (miRNAs), circular RNAs (circRNAs), and other regulatory RNAs. The isolation and quantitative analyses of diverse types of RNAs in neurons are critical to understand not only the posttranscriptional mechanisms regulating mRNA levels and their translation but also the potential of several RNAs expressed in the same neurons to regulate these processes by generating networks of competing endogenous RNAs (ceRNAs). This chapter will describe methods for the isolation and analyses of circRNA and miRNA levels from the same brain tissue sample | ||
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| 650 | 4 | |a RNA isolation | |
| 650 | 4 | |a circRNAs | |
| 650 | 4 | |a miRNAs | |
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