Hepatic and serum branched-chain fatty acid profile in patients with nonalcoholic fatty liver disease : A case-control study
© 2023 The Authors. Obesity published by Wiley Periodicals LLC on behalf of The Obesity Society..
OBJECTIVE: Alterations in the hepatic lipidome are a crucial factor involved in the pathophysiology of nonalcoholic fatty liver disease (NAFLD). The aim of this study was to evaluate the serum and hepatic profile of branched-chain fatty acids (BCFAs) in patients with different stages of NAFLD.
METHODS: This was a case-control study performed in 27 patients without NAFLD, 49 patients with nonalcoholic fatty liver, and 17 patients with nonalcoholic steatohepatitis, defined by liver biopsies. Serum and hepatic levels of BCFAs were analyzed by gas chromatography-mass spectrometry. The hepatic expression of genes involved in the endogenous synthesis of BCFAs was analyzed by real-time quantitative polymerase chain reaction (RT-qPCR).
RESULTS: A significant increase in hepatic BCFAs was found in subjects with NAFLD compared with those without NAFLD; no differences were observed in serum BCFAs between study groups. Trimethyl BCFAs, iso-BCFAs, and anteiso-BCFAs were increased in subjects with NAFLD (either nonalcoholic fatty liver or nonalcoholic steatohepatitis) compared with those without NAFLD. Correlation analysis showed a relationship between hepatic BCFAs and the histopathological diagnosis of NAFLD, as well as other histological and biochemical parameters related to this disease. Gene expression analysis in liver showed that the mRNA levels of BCAT1, BCAT2, and BCKDHA were upregulated in patients with NAFLD.
CONCLUSIONS: These results suggest that the increased production of liver BCFAs might be related to NAFLD development and progression.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:31 |
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| Enthalten in: |
Obesity (Silver Spring, Md.) - 31(2023), 4 vom: 14. Apr., Seite 1064-1074 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Martínez-Montoro, José Ignacio [VerfasserIn] |
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| Links: |
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| Themen: |
BCAT1 protein, human |
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| Anmerkungen: |
Date Completed 22.03.2023 Date Revised 23.04.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1002/oby.23711 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM353824895 |
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| 100 | 1 | |a Martínez-Montoro, José Ignacio |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Hepatic and serum branched-chain fatty acid profile in patients with nonalcoholic fatty liver disease |b A case-control study |
| 264 | 1 | |c 2023 | |
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| 500 | |a Date Revised 23.04.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2023 The Authors. Obesity published by Wiley Periodicals LLC on behalf of The Obesity Society. | ||
| 520 | |a OBJECTIVE: Alterations in the hepatic lipidome are a crucial factor involved in the pathophysiology of nonalcoholic fatty liver disease (NAFLD). The aim of this study was to evaluate the serum and hepatic profile of branched-chain fatty acids (BCFAs) in patients with different stages of NAFLD | ||
| 520 | |a METHODS: This was a case-control study performed in 27 patients without NAFLD, 49 patients with nonalcoholic fatty liver, and 17 patients with nonalcoholic steatohepatitis, defined by liver biopsies. Serum and hepatic levels of BCFAs were analyzed by gas chromatography-mass spectrometry. The hepatic expression of genes involved in the endogenous synthesis of BCFAs was analyzed by real-time quantitative polymerase chain reaction (RT-qPCR) | ||
| 520 | |a RESULTS: A significant increase in hepatic BCFAs was found in subjects with NAFLD compared with those without NAFLD; no differences were observed in serum BCFAs between study groups. Trimethyl BCFAs, iso-BCFAs, and anteiso-BCFAs were increased in subjects with NAFLD (either nonalcoholic fatty liver or nonalcoholic steatohepatitis) compared with those without NAFLD. Correlation analysis showed a relationship between hepatic BCFAs and the histopathological diagnosis of NAFLD, as well as other histological and biochemical parameters related to this disease. Gene expression analysis in liver showed that the mRNA levels of BCAT1, BCAT2, and BCKDHA were upregulated in patients with NAFLD | ||
| 520 | |a CONCLUSIONS: These results suggest that the increased production of liver BCFAs might be related to NAFLD development and progression | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
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| 650 | 7 | |a BCAT1 protein, human |2 NLM | |
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| 700 | 1 | |a Martinez-Sanchez, María Antonia |e verfasserin |4 aut | |
| 700 | 1 | |a Balaguer-Román, Andrés |e verfasserin |4 aut | |
| 700 | 1 | |a Fernández-Ruiz, Virginia E |e verfasserin |4 aut | |
| 700 | 1 | |a Ferrer-Gómez, Mercedes |e verfasserin |4 aut | |
| 700 | 1 | |a Sledzinski, Tomasz |e verfasserin |4 aut | |
| 700 | 1 | |a Frutos, María Dolores |e verfasserin |4 aut | |
| 700 | 1 | |a Fernández-García, José Carlos |e verfasserin |4 aut | |
| 700 | 1 | |a Mika, Adriana |e verfasserin |4 aut | |
| 700 | 1 | |a Ramos-Molina, Bruno |e verfasserin |4 aut | |
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