Deregulation of host gene expression by HPV16 E8^E2 knock-out genomes is due to increased productive replication
Copyright © 2023 Elsevier Inc. All rights reserved..
Productive replication of human papillomaviruses (HPV) only takes place in differentiating keratinocytes. The HPV16 E8^E2 protein acts as a repressor of viral gene expression and genome replication and HPV16 E8^E2 knock-out (E8-) genomes display enhanced viral late protein expression in differentiated cells. Global transcriptome analysis of differentiated HPV16 wild-type and E8-cell lines revealed a small number of differentially expressed genes which are not related to cell cycle, DNA metabolism or keratinocyte differentiation. The analysis of selected genes suggested that deregulation requires cell differentiation and positively correlated with the expression of viral late, not early transcripts. Consistent with this, the additional knock-out of the viral E4 and E5 genes, which are known to enhance productive replication, attenuated the deregulation of these host cell genes. In summary, these data reveal that productive HPV16 replication modulates host cell transcription.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:581 |
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| Enthalten in: |
Virology - 581(2023) vom: 10. Apr., Seite 39-47 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kuehner, Franziska [VerfasserIn] |
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| Links: |
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| Themen: |
E8^E2 |
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| Anmerkungen: |
Date Completed 03.04.2023 Date Revised 04.05.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.virol.2023.02.007 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Copyright © 2023 Elsevier Inc. All rights reserved. | ||
| 520 | |a Productive replication of human papillomaviruses (HPV) only takes place in differentiating keratinocytes. The HPV16 E8^E2 protein acts as a repressor of viral gene expression and genome replication and HPV16 E8^E2 knock-out (E8-) genomes display enhanced viral late protein expression in differentiated cells. Global transcriptome analysis of differentiated HPV16 wild-type and E8-cell lines revealed a small number of differentially expressed genes which are not related to cell cycle, DNA metabolism or keratinocyte differentiation. The analysis of selected genes suggested that deregulation requires cell differentiation and positively correlated with the expression of viral late, not early transcripts. Consistent with this, the additional knock-out of the viral E4 and E5 genes, which are known to enhance productive replication, attenuated the deregulation of these host cell genes. In summary, these data reveal that productive HPV16 replication modulates host cell transcription | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a E8^E2 | |
| 650 | 4 | |a HPV16 | |
| 650 | 4 | |a Keratinocyte differentiation | |
| 650 | 4 | |a Papillomavirus | |
| 650 | 4 | |a Replication cycle | |
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| 700 | 1 | |a Straub, Elke |e verfasserin |4 aut | |
| 700 | 1 | |a Iftner, Thomas |e verfasserin |4 aut | |
| 700 | 1 | |a Stubenrauch, Frank |e verfasserin |4 aut | |
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