An antisense intragenic lncRNA SEAIRa mediates transcriptional and epigenetic repression of SERRATE in Arabidopsis
SERRATE (SE) is a core protein for microRNA (miRNA) biogenesis as well as for mRNA alternative splicing. Investigating the regulatory mechanism of SE expression is hence critical to understanding its detailed function in diverse biological processes. However, little about the control of SE expression has been clarified, especially through long noncoding RNA (lncRNA). Here, we identified an antisense intragenic lncRNA transcribed from the 3' end of SE, named SEAIRa. SEAIRa repressed SE expression, which in turn led to serrated leaves. SEAIRa recruited plant U-box proteins PUB25/26 with unreported RNA binding ability and a ubiquitin-like protein related to ubiquitin 1 (RUB1) for H2A monoubiquitination (H2Aub) at exon 11 of SE. In addition, PUB25/26 helped cleave SEAIRa and release the 5' domain fragment, which recruited the PRC2 complex for H3 lysine 27 trimethylation (H3K27me3) deposition at the first exon of SE. The distinct modifications of H2Aub and H3K27me3 at different sites of the SE locus cooperatively suppressed SE expression. Collectively, our results uncover an epigenetic mechanism mediated by the lncRNA SEAIRa that modulates SE expression, which is indispensable for plant growth and development.
| Errataetall: |
ErratumIn: Proc Natl Acad Sci U S A. 2023 Aug 22;120(34):e2312508120. - PMID 37579181 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:120 |
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| Enthalten in: |
Proceedings of the National Academy of Sciences of the United States of America - 120(2023), 10 vom: 07. März, Seite e2216062120 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Chen, Wei [VerfasserIn] |
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| Links: |
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| Themen: |
Arabidopsis Proteins |
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| Anmerkungen: |
Date Completed 06.03.2023 Date Revised 02.09.2023 published: Print-Electronic ErratumIn: Proc Natl Acad Sci U S A. 2023 Aug 22;120(34):e2312508120. - PMID 37579181 Citation Status MEDLINE |
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| doi: |
10.1073/pnas.2216062120 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM353633445 |
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| 100 | 1 | |a Chen, Wei |e verfasserin |4 aut | |
| 245 | 1 | 3 | |a An antisense intragenic lncRNA SEAIRa mediates transcriptional and epigenetic repression of SERRATE in Arabidopsis |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
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| 500 | |a Date Revised 02.09.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a ErratumIn: Proc Natl Acad Sci U S A. 2023 Aug 22;120(34):e2312508120. - PMID 37579181 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a SERRATE (SE) is a core protein for microRNA (miRNA) biogenesis as well as for mRNA alternative splicing. Investigating the regulatory mechanism of SE expression is hence critical to understanding its detailed function in diverse biological processes. However, little about the control of SE expression has been clarified, especially through long noncoding RNA (lncRNA). Here, we identified an antisense intragenic lncRNA transcribed from the 3' end of SE, named SEAIRa. SEAIRa repressed SE expression, which in turn led to serrated leaves. SEAIRa recruited plant U-box proteins PUB25/26 with unreported RNA binding ability and a ubiquitin-like protein related to ubiquitin 1 (RUB1) for H2A monoubiquitination (H2Aub) at exon 11 of SE. In addition, PUB25/26 helped cleave SEAIRa and release the 5' domain fragment, which recruited the PRC2 complex for H3 lysine 27 trimethylation (H3K27me3) deposition at the first exon of SE. The distinct modifications of H2Aub and H3K27me3 at different sites of the SE locus cooperatively suppressed SE expression. Collectively, our results uncover an epigenetic mechanism mediated by the lncRNA SEAIRa that modulates SE expression, which is indispensable for plant growth and development | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a SE | |
| 650 | 4 | |a epigenetic modification | |
| 650 | 4 | |a gene regulation | |
| 650 | 4 | |a lncRNA | |
| 650 | 7 | |a Histones |2 NLM | |
| 650 | 7 | |a RNA, Long Noncoding |2 NLM | |
| 650 | 7 | |a SE protein, Arabidopsis |2 NLM | |
| 650 | 7 | |a Arabidopsis Proteins |2 NLM | |
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| 700 | 1 | |a Zhu, Tao |e verfasserin |4 aut | |
| 700 | 1 | |a Shi, Yining |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Ying |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Wei Jian |e verfasserin |4 aut | |
| 700 | 1 | |a Chan, Ru Jing |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Dijun |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Wenli |e verfasserin |4 aut | |
| 700 | 1 | |a Yuan, Yuren Adam |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xiue |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Bo |e verfasserin |4 aut | |
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